FlyBase Allele Report: Hsap\RELA[AD.R52F12]

General Information

Symbol

Hsap\RELA^AD.R52F12

Species

H. sapiens

Name

FlyBase ID

FBal0329013

Feature type

allele

Associated gene

Hsap\RELA

Associated Insertion(s)

Carried in Construct

P{R52F12-p65.AD}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Rubin Laboratory at Janelia Farm, 2017)

Progenitor genotype

Carried in construct

P{R52F12-p65.AD}

Cytology

Description

The GMR52F12 enhancer fragment followed by the Drosophila synthetic core promoter (DSCP) is fused upstream of the p65(AD)::Zip+ hemidriver (component of a 'split driver' system, contains the p65 activation domain).

(Rubin Laboratory at Janelia Farm, 2017)

Allele components

Component

Use(s)

Regulatory region(s)

GMR52F12

Encoded product / tool

p65(AD)::Zip+

split driver - transcription activation fragment

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

zone C/E Johnston organ neuron

Comment: when combined with P{R60E02-GAL4.DBD}

(Hampel et al., 2015)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal grooming behavior | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^DBD.R60E02

(Hampel et al., 2015)

abnormal grooming behavior | heat sensitive, with Scer\GAL4^DBD.R60E02, TrpA1^UAS.(B).cKa

(Hampel et al., 2015)

Phenotype Manifest In

Detailed Description

Statement

Reference

Thermogenetic activation (using TrpA1^{Scer\UAS.(B).cKa} at 30-31[o]C) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R60E02 and Hsap\RELA^AD.R52F12 results in a significant increase of antennal grooming behavior, compared to controls.

Optogenetic activation (using Cnoc\ChR1^{Cs.IVS.20xUAS.Venus} with red light activation) of neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R60E02 and Hsap\RELA^AD.R52F12 induces antennal grooming, which persists for tens of seconds beyond red light cessation.

(Hampel et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Ctet\tetX^TNT-LC.UAS, Hsap\RELA^AD.R52F12, Scer\GAL4^DBD.R60E02 is a suppressor | partially of abnormal grooming behavior | heat sensitive phenotype of Ecol\lexA^GMR27H08, TrpA1^{13xlexAop.JFRC26}

(Hampel et al., 2015)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Inhibition of synaptic release (using Ctet\TeTxLC^TNT.Scer\UAS) in neurons targeted by the split-GAL4 pair of Scer\GAL4^DBD.R60E02 and Hsap\RELA^AD.R52F12 significantly partially suppresses the increase in antennal grooming seen in flies with thermogenetic activation (using TrpA1^{13xlexAop.JFRC26} at 30-31[o]C) of Ecol\lexA^GMR27H08 neurons.

(Hampel et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (5)

Bloomington

71084

w¹¹¹⁸; P{R52F12-p65.AD}attP40

89201

w¹¹¹⁸; P{R52F12-p65.AD}attP40; P{R72F09-GAL4.DBD}attP2