FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Rab3FRT.F3.2XHA
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General Information
Symbol
Dmel\Rab3FRT.F3.2XHA
Species
D. melanogaster
Name
FlyBase ID
FBal0346794
Feature type
allele
Associated gene
Dmel\Rab3
Associated Insertion(s)
TI{TI}Rab3FRT.F3.2XHA
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Williams et al., 2019)
gene targeting by homologous recombination
(Williams et al., 2019)
Progenitor genotype
Associated Insertion(s)
TI{TI}Rab3FRT.F3.2XHA
Cytology
Description

The endogenous Rab3 locus has been modified so that exon 2 is tagged at the N-terminal end with two copies of the Tag:HA epitope, and this tagged exon is inverted relative to the rest of the transcription unit, preventing expression of Rab3 protein. The inverted exon is surrounded by two pairs of incompatible recombinase target sites (FRT and FRT3) which are oriented such that the inverted exon can be stably inverted by expression of FLP recombinase (an odd number of inversions followed by an excision results in the exon being in the correct orientation to be expressed and being flanked by a single FRT3 and a single FRT site, which cannot recombine with each other).

(Williams et al., 2019)
Allele components
Component
Use(s)
Also carries
FRT
FRT3
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Rab3FRT.F3.2XHA
      Name Synonyms
      Secondary FlyBase IDs
        References (1)