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| Term | CRISPR/Cas9 | ID (Ontology) | FBcv:0003008 (FlyBase CV) | |||||||||
| Definition | Sequence change caused by a cleavage of a specific DNA sequence induced by CRISPR (clustered regularly interspaced short palindromic repeats)/Cas9 nuclease system. This system relies on the transcription of a chimeric guide RNA (gRNA) which directs the Cas9 endonuclease to a specified DNA sequence through basepairing to 20 nucleotide CRISPR target sites. These sites are located next to a 3 nucleotide protospacer adjacent motif (PAM) required for DNA cleavage.[ FlyBase:FBrf0222128 FlyBase:FBrf0222292 FlyBase:FBrf0222617 ] | |||||||||||
| Also Known As | "change induced by CRISPR/Cas9 endonuclease cleavage" | |||||||||||
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Full annotation statements including this term (annotations to child terms are NOT included), and relevant FlyBase records
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origin of mutation |__site specific cleavage |__CRISPR/Cas9 18026 rec. |
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| Is a | site specific cleavage | ||
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