FlyBase Allele Report: Dmel\mtd[HMS01666]

General Information

Symbol

Dmel\mtd^HMS01666

Species

D. melanogaster

Name

FlyBase ID

FBal0278784

Feature type

allele

Associated gene

Dmel\mtd

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS01666}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2010.12.1)

Mutagen

in vitro construct

(Ni et al., 2010.12.1)

Progenitor genotype

Carried in construct

P{TRiP.HMS01666}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2010.12.1)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS01666

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cerebellar hyplasia/atrophy, epilepsy, and global developmental delay

CEA

(Wang et al., 2019)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^elav.PLu

(Wang et al., 2019)

lethal - all die before end of P-stage, with Scer\GAL4^Act5C.PI

(Wang et al., 2019)

short lived, with Scer\GAL4^elav.PLu

(Wang et al., 2019)

short lived, with Scer\GAL4^nSyb.PP

(Wang et al., 2019)

Phenotype Manifest In

adult brain, with Scer\GAL4^elav.PLu

(Wang et al., 2019)

autophagosome | adult stage, with Scer\GAL4^elav.PLu

(Wang et al., 2019)

lysosome | adult stage, with Scer\GAL4^elav.PLu

(Wang et al., 2019)

lysosome | adult stage | progressive, with Scer\GAL4^elav.PLu

(Wang et al., 2019)

photoreceptor cell R7, with Scer\GAL4^elav.PLu

(Wang et al., 2019)

Detailed Description

Statement

Reference

9-days old adults expressing mtd^HMS01666 under the control of Scer\GAL4^elav.PLu show severe neuronal loss and vacuolization (HE staining), as well as a progressive increase in lysosomes (AO staining). In the retinae, only a subset of R7 photoreceptors display aberrant morphological features, while the other photoreceptors and pigment glia appear normal at days 5 and 8; numerous R7 photoreceptors display highly aberrant shapes and dark cytoplasm typical of unhealthy photoreceptors; these photoreceptors also accumulate numerous autophagosome structures with abnormal undigested contents.

Expression under the control of Scer\GAL4^repo or Scer\GAL4^C57 does not result in any obvious phenotypes.

(Wang et al., 2019)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^elav.PLu, mtd^HMS01666 has short lived phenotype, suppressible | partially by Hsap\OXR1^UAS.Tag:HA, Scer\GAL4^elav.PLu

(Wang et al., 2019)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

38519

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01666}attP2/TM3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

mtd^HMS01666

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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