FlyBase Allele Report: Spyo\Cas9[UAS.cUa]

General Information

Symbol

Spyo\Cas9^UAS.cUa

Species

S. pyogenes

Name

UAS construct a of Unknown

FlyBase ID

FBal0346334

Feature type

allele

Associated gene

Spyo\Cas9

Associated Insertion(s)

Carried in Construct

P{UAS-Cas9.U}

Also Known As

UAS-CAS9, UAS-dCas9

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(FlyBase Curators, 2025-)

Progenitor genotype

Carried in construct

P{UAS-Cas9.U}

Cytology

Description

UAS regulatory sequences drive expression of Cas9.

(FlyBase Curators, 2025-)

FlyBase curator comment: this entry represents a transgenic construct where the particular construct used cannot be determined.

(FlyBase Curators, 2025-)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Cas9

RNA-guided nuclease

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell number | larval stage, with Scer\GAL4^dome-PG14, whd^TKO.GS00854

(Tiwari et al., 2020)

abnormal circadian rhythm | adult stage | progressive, with Lkrsdh^TOE.GS01508, Scer\GAL4^{Lkrsdh-CR70173-TG4.0}

(Lv et al., 2024)

abnormal circadian rhythm | adult stage | progressive, with Lkrsdh^TOE.GS01508, Scer\GAL4^nSyb.PP

(Lv et al., 2024)

abnormal circadian rhythm | adult stage | progressive, with Lkrsdh^TOE.GS01508, Scer\GAL4^repo.PU

(Lv et al., 2024)

abnormal locomotor behavior | adult stage, with FoxP^UAS.4xgRNA, Scer\GAL4^elav.PU

(Palazzo et al., 2020)

abnormal locomotor behavior | adult stage, with FoxP^UAS.4xgRNA, Scer\GAL4^futsch-C380

(Palazzo et al., 2020)

abnormal locomotor behavior | adult stage, with FoxP^UAS.4xgRNA, Scer\GAL4^GMR55G08

(Palazzo et al., 2020)

abnormal locomotor behavior | adult stage, with FoxP^UAS.4xgRNA, Scer\GAL4^Toll-6-D42

(Palazzo et al., 2020)

abnormal size | larval stage, with Scer\GAL4^dome-PG14, whd^TKO.GS00854

(Tiwari et al., 2020)

decreased efficacy of learning | adult stage, with Scer\GAL4^FoxP-iB-Gal4, aPKC^TKO.GS04189

(Ehweiner et al., 2024)

decreased efficacy of learning | adult stage, with Scer\GAL4^futsch-C380, aPKC^TKO.GS04189

(Ehweiner et al., 2024)

decreased efficacy of learning | adult stage | RU486 conditional, with FoxP^UAS.4xgRNA, Scer\GAL4^GS.nSyb.PB

(Ehweiner et al., 2024)

decreased rate of movement | adult stage, with FoxP^UAS.4xgRNA, Scer\GAL4^elav.PU

(Palazzo et al., 2020)

decreased rate of movement | adult stage, with FoxP^UAS.4xgRNA, Scer\GAL4^futsch-C380

(Palazzo et al., 2020)

decreased rate of movement | adult stage, with FoxP^UAS.4xgRNA, Scer\GAL4^GMR55G08

(Palazzo et al., 2020)

decreased rate of movement | adult stage, with FoxP^UAS.4xgRNA, Scer\GAL4^Toll-6-D42

(Palazzo et al., 2020)

visible | embryonic stage, with Raf^TKO.GS00615, Scer\GAL4^nanos.PU

(Lusk et al., 2022)

Phenotype Manifest In

cortical zone of lymph gland primary lobe | larval stage, with Scer\GAL4^dome-PG14, whd^TKO.GS00854

(Tiwari et al., 2020)

embryonic/first instar larval cuticle | embryonic stage, with Raf^TKO.GS00615, Scer\GAL4^nanos.PU

(Lusk et al., 2022)

ventral denticle belt | absent | embryonic stage, with Raf^TKO.GS00615, Scer\GAL4^nanos.PU

(Lusk et al., 2022)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Scer\GAL4^nub-AC-62, Spyo\Cas9^UAS.cUa, Zzzz\VHH^{GFP4.UAS.Tag:ER(KDEL)}, wls^ExGFP, wls^HD_CFD01954 has visible | adult stage phenotype

(Yu et al., 2020)

Phenotype Manifest In

Other

Statement

Reference

Scer\GAL4^nub-AC-62, Spyo\Cas9^UAS.cUa, Zzzz\VHH^{GFP4.UAS.Tag:ER(KDEL)}, wls^ExGFP, wls^HD_CFD01954 has wing margin | absent phenotype

(Yu et al., 2020)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^{Lkrsdh-CR70173-TG4.0}, Lkrsdh^TOE.GS01508, Spyo\Cas9^UAS.cUa rescues Lkrsdh^MB01942

(Lv et al., 2024)

Fails to rescue

Scer\GAL4^nSyb.PP, Lkrsdh^TOE.GS01508, Spyo\Cas9^UAS.cUa fails to rescue Lkrsdh^MB01942

(Lv et al., 2024)

Lkrsdh^TOE.GS01508, Scer\GAL4^repo.PU, Spyo\Cas9^UAS.cUa fails to rescue Lkrsdh^MB01942

(Lv et al., 2024)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Spyo\Cas9^UAS.cUa

Name Synonyms

UAS construct a of Unknown

Secondary FlyBase IDs

References (24)

Report Sections

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