hsrω, hsr-omega, Hsromega, hsr-ω, er3
Gene model based, in part, on work in FBrf0213860.
Gene model reviewed during 5.47
miRNA(s) located within the transcribed region of this non-coding RNA gene.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.41
Gene model reviewed during 5.45
Gene model reviewed during 6.02
Transcripts contain a putative 27 aa CDS that is conserved, and the transcripts fractionate with mono-ribosomes, but the putative protein encoded could not be detected (Fini et al., 1989. FBrf0050187).
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\lncRNA:Hsrω using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\lncRNA:Hsrω in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: lncRNA:Hsr ω Hsrω
New annotation (CR31400) in release 3 of the genome annotation.
Functional RNA molecules transcribed from Hsrω are an important and polymorphic regulatory component of an insect thermoresistance phenotype. Both molecular allelic variation and expression characteristics are closely associated with knockdown heat resistance and with the hardening response of flies.
Hsrω cis-acting sequences that regulate developmental activity in different tissues are studied using Ecol\lacZ reporter gene constructs. Hsrω is expressed in almost all tissue types and is expressed in a specific spatial pattern in different cells of a given tissue. Results suggest a region between -346bp and -844bp upstream of the gene contains major regulatory elements for developmental expression of the gene in most larval and adult tissues.
Hsrω transcript is broadly distributed over the nucleus in both heat shock and control cells. Polyadenylation of Hsrω RNA utilises RNA processing signals typical of pre-mRNA. Levels of Hsrω are regulated by both transcription and turnover, regulation is sensitive to actinomycin D.
The product of Hsp83 and its homologs shows a specific nuclear localization in different species of Drosophila and Chironomus. Besides being abundant in the cytoplasm, the Hsp83 product is associated with specific chromosomal loci, such as Hsrω and Dhyd\Hsrω, the telomeric Balbiani rings in Chironomus thummi and the heat-induced puff I-1C in C.tentans.
Nascent chain nuclear run-on assays in KC161 cells reveal different responses to heat shock for different genes. Transcription of His1 is severely inhibited under mild heat shocks, of Act5C decreases proportionally with increasing temperature while that of the core histone genes or the heat shock cognates is repressed only under extreme heat shock. In unshocked cells Hsp83 is moderately transcribed while transcription from the other heat shock genes is undetectable. Engaged but paused RNA molecules are found at the various Hsp70 and Hsp26 genes but not at the other heat shock genes. Increased transcription of the heat shock genes is observed within 1-2 mins of heat shock and maximal rates were reached within 2-5 minutes. Rates of transcription vary over a 20-fold range. Hsrω is transcribed at a very high rate under non-heat shock conditions, and its response to elevated temperatures is different from that of the protein coding heat shock genes.
Hsrω RNA expression in non-stressed cells during development has been analysed.
Nuclear and cytoplasmic transcript together play a role in coordinating nuclear and cytoplasmic activity.
A sequence that responds to heat shock by generation of a large puff in polytene chromosomes. The 93D region transcribes mRNA that apparently is not translated. Hsrω is active in almost all cells in D.melanogaster; the activity is greatly increased by heat shock (Bonner and Pardue, 1976) and is induced independently by benzamide (Lakhotia and Mukherjee, 1970). The inducibility of the locus is selectively repressed by a combination of heat shock with another inducer, by rearing larvae at 10oC, by heterozygous deficiency for 93D or by treating wild-type salivaries with β-alanine (Lakhotia, 1989). The 93D heat shock mRNA is predominantly poly(A)- in the cytoplasm, whereas nuclear transcripts are both poly(A)+ and poly(A)- (Lengyel et al., 1980). Heat-shock response in homozygous deficiencies for Hsrω is indistinguishable from normal except for the absence of the 93D puff and transcripts.