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| Term | electrophoretic mobility shift assay | ID (Ontology) | MI:0413 (Molecular Interactions) | |||||
| Definition | This method proves the interaction between a nucleic acid and a protein partner. On the same electrophoresis gel 1 lane is loaded with a nucleic acid of known sequence, a second lane is loaded with the same nucleic acid together with a purified protein (or a protein mixture). The nucleic acid is often radio-labelled to enable visualisation by autoradiography. Comparison of the nucleic acid migration in the two lanes enables the retardation of the nucleic acid due to its interaction with a protein to be observed.[ PubMed:12169687 ] | |||||||
| Also Known As | "band shift" ; "emsa" ; "Gel retardation assay" | |||||||
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electrophoretic mobility-based method |__comigration in gel electrophoresis |__electrophoretic mobility shift assay 204 rec. |__electrophoretic mobility supershift assay 19 rec. |
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| Is a | comigration in gel electrophoresis | ||
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