FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\lncRNA:HsrωEP93D
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General Information
Symbol
Dmel\lncRNA:HsrωEP93D
Species
D. melanogaster
Name
FlyBase ID
FBal0194560
Feature type
allele
Associated gene
Dmel\lncRNA:Hsrω
Associated Insertion(s)
P{EP}lncRNA:HsrωEP93D
Carried in Construct
Also Known As
EP93D
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
P-element activity
(Sengupta and Lakhotia, 2006)
Progenitor genotype
P{PZ}lncRNA:Hsrω05241
(Mallik and Lakhotia, 2009)
lncRNA:Hsrω05241
(Sengupta and Lakhotia, 2006)
Associated Insertion(s)
P{EP}lncRNA:HsrωEP93D
Cytology
Description

Insertion at -130bp position in the Hsrω promoter.

(Mallik and Lakhotia, 2009)

Carries a P{EP} insertion at the same site as the P{PZ} insertion in Hsrω05241, from which it was generated by P-transposon swapping.

(Sengupta and Lakhotia, 2006)
Allele components
Component
Use(s)
Inserted element
P{EP}
Regulatory region(s)
UAS
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of HsrωEP93D under the control of Scer\GAL4GMR.PS results in rough eye phenotype in adult flies.

      (Lo Piccolo and Yamaguchi, 2017)

      The synaptic morphology at lncRNA:HsrωEP93D larval muscle 6/7 NMJs is comparable to controls.

      (Choudhury et al., 2016)

      Expression of a single copy of lncRNA:HsrωEP93D under the control of Scer\GAL4dpp.blk1 causes a mild disruption of photoreceptor cells; expression of two copies causes high mortality during development and severe disruption of photoreceptors.

      Expression of a single copy of lncRNA:HsrωEP93D under the control of Scer\GAL4elav-C155 has no effect on lifespan.

      Expression of a single copy of lncRNA:HsrωEP93D under the control of Scer\GAL4ptc-559.1 causes extra or missing scutellar bristles; expression of two copies additionally causes the notum to be deformed.

      Expression of a single copy of lncRNA:HsrωEP93D under the control of Scer\GAL4C805 causes partial lethality during the P-stage and an increase in the proportion of female adults; expression of two copies causes greater lethality, with most dying as fully differentiated pharates, and wing blistering.

      Expression of lncRNA:HsrωEP93D under the control of Scer\GAL4c825 does not result in sex comb defects.

      Expression of lncRNA:HsrωEP93D under the control of Scer\GAL4Act5C.PI causes a majority of animals to die before adult stage, with higher mortality in lncRNA:HsrωEP93D homozygotes than heterozygotes; surviving adults are fertile, but the gender ratio is skewed in favor of females. Expression of lncRNA:HsrωEP93D under the control of Scer\GAL4Act5C.PI also results in fewer, larger omega speckles in the nucleus.

      (Mallik and Lakhotia, 2011)

      Eyes of HsrωEP93D homozygous flies show disorganisation of ommatidial units.

      (Sengupta and Lakhotia, 2006)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Suppressed by
      Enhancer of
      NOT Enhancer of
      Suppressor of
      NOT Suppressor of
      Statement
      Reference

      Scer\GAL4elav-C155, lncRNA:HsrωEP93D, lncRNA:Hsromega [+] is a non-suppressor of some die during P-stage | dominant phenotype of elavC155

      (Mallik and Lakhotia, 2011)

      Scer\GAL4GMR.PF/lncRNA:HsrωEP93D is a non-suppressor of lethal | pupal stage phenotype of Scer\GAL4GMR.PF, nejΔQ.UAS

      (Mallik and Lakhotia, 2010)
      Other
      Phenotype Manifest In
      Enhanced by
      Suppressed by
      Enhancer of
      Statement
      Reference

      Scer\GAL4GMR.PS/lncRNA:HsrωEP93D is an enhancer of eye phenotype of Scer\GAL4GMR.PS, cazRNAi.UAS.cUa

      (Lo Piccolo and Yamaguchi, 2017)

      Scer\GAL4GMR.PS/lncRNA:HsrωEP93D is an enhancer of ommatidium | adult stage phenotype of Scer\GAL4GMR.PS, cazRNAi.UAS.cUa

      (Lo Piccolo and Yamaguchi, 2017)

      lncRNA:HsrωEP93D/lncRNA:HsrωEP93D is an enhancer of eye phenotype of Scer\GAL4GMR.PU

      (Mallik and Lakhotia, 2011)

      Scer\GAL4Act5C.PI/lncRNA:HsrωEP93D is an enhancer of polytene chromosome phenotype of Iswiunspecified

      (Onorati et al., 2011)

      Scer\GAL4GMR.PF/lncRNA:HsrωEP93D is an enhancer of eye phenotype of Scer\GAL4GMR.PF, nejF2161A.UAS.Tag:V5

      (Mallik and Lakhotia, 2010)

      Scer\GAL4GMR.PF/lncRNA:HsrωEP93D is an enhancer of eye phenotype of Scer\GAL4GMR.PF, nejΔNZK.UAS

      (Mallik and Lakhotia, 2010)

      Scer\GAL4GMR.PF/lncRNA:HsrωEP93D is an enhancer of eye phenotype of Scer\GAL4GMR.PF, nejRNAi.UAS.cKa

      (Mallik and Lakhotia, 2010)

      lncRNA:HsrωEP93D, Scer\GAL4GMR.PF is an enhancer of eye phenotype of Df(3R)Hrb87F/+, Scer\GAL4GMR.PF, Zzzz\CAG127Q.UAS.Tag:HA

      (Mallik and Lakhotia, 2010)

      Scer\GAL4GMR.PF/lncRNA:HsrωEP93D is an enhancer of eye phenotype of Scer\GAL4GMR.PF, Zzzz\CAG127Q.UAS.Tag:HA, nejF2161A.UAS.Tag:V5

      (Mallik and Lakhotia, 2010)

      lncRNA:HsrωEP93D, Scer\GAL4GMR.PF is an enhancer of eye phenotype of Diap1RNAi.UAS.cLa, Scer\GAL4GMR.PF

      (Mallik and Lakhotia, 2009)

      lncRNA:HsrωEP93D/lncRNA:HsrωEP93D is an enhancer of eye phenotype of Scer\GAL4GMR.PF

      (Mallik and Lakhotia, 2009)

      lncRNA:HsrωEP93D/lncRNA:HsrωEP93D is an enhancer of photoreceptor phenotype of Scer\GAL4GMR.PF

      (Mallik and Lakhotia, 2009)

      Scer\GAL4GMR.PF/lncRNA:HsrωEP93D is an enhancer of eye phenotype of Scer\GAL4GMR.PF, egrUAS.cIa

      (Mallik and Lakhotia, 2009)

      Scer\GAL4GMR.PF/lncRNA:HsrωEP93D is an enhancer of eye phenotype of Scer\GAL4GMR.PF, Tak1UAS.cTa

      (Mallik and Lakhotia, 2009)

      Scer\GAL4GMR.PF/lncRNA:HsrωEP93D is an enhancer of eye phenotype of Scer\GAL4GMR.PF, Zzzz\CAG127Q.UAS.Tag:HA

      (Mallik and Lakhotia, 2009, Sengupta and Lakhotia, 2006)
      NOT Enhancer of
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The eye morphology defects (rough appearance with fused ommatidia) characteristic for flies expressing either cazVDRC.cUa or HsrωEP93D individually are further enhanced when the two transgenes are co-expressed.

      (Lo Piccolo and Yamaguchi, 2017)

      Expression of HsrωEP93D under the control of Scer\GAL4Act5C.PI enhances the polytene chromosome condensation defects of Iswiunspecified mutants.

      (Onorati et al., 2011)

      Coexpression of one copy of HsrωEP93D in the Scer\GAL4GMR.PF, nejF2161A.Scer\UAS.T:SV5\V5 background exaggerates the eye damage.

      Co-expression of HsrωEP93D in the Scer\GAL4GMR.PF, nejdsRNA.Scer\UAS.cKa background enhances eye degeneration with the loss of ommatidial integrity extending to most of the eye.

      Co-expression of HsrωEP93D enhances the eye damage seen in Scer\GAL4GMR.PF, nejΔNZK.Scer\UAS flies.

      (Mallik and Lakhotia, 2010)
      Xenogenetic Interactions
      Statement
      Reference

      The rough eye phenotype seen in the absence of any responder transgene in animals carrying two copies of Scer\GAL4GMR.PU is enhanced by the addition of lncRNA:HsrωEP93D/lncRNA:HsrωEP93D.

      (Mallik and Lakhotia, 2011)

      Expression of HsrωEP93D in the Scer\GAL4GMR.PF, Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1, Df(3R)Hrb87F/+ background significantly enhances the appearence of black lesions on the eye surface, although it has no effect on mortality.

      Expression of nejF2161A.Scer\UAS.T:SV5\V5 in the Scer\GAL4GMR.PF, Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 background results in 23% lethality at the pupal stage. Surviving flies show a reduction in eye size and a greater disruption in ommatidial arrays. Additional co-expression of HsrωEP93D results in a greater reduction in eye size, near complete loss of ommatidial arrays and bristles and the appearence of black lesions on the eye surface. Co-expression of HsrωEP93D does not enhance pupal death.

      (Mallik and Lakhotia, 2010)

      Expression of two copies of HsrωEP93D enhances the rough eye, photoreceptor degeneration, and increased apoptosis phenotypes seen in Scer\GAL4GMR.PF/Scer\GAL4GMR.PF flies.

      Co-expression of HsrωEP93D leads to a slight enhancement of the eye phenotype in Scer\GAL4GMR.PF,thdsRNA.Scer\UAS.cLa flies. Co-expression of HsrωEP93D suppresses the pupal lethality of Scer\GAL4GMR.PF,thdsRNA.Scer\UAS.cLa flies.

      Coexpression of HsrωEP93D enhances the Scer\GAL4GMR.PF, egrScer\UAS.cIa eye phenotype, leading to severe head malformations and the formation of black lesions on the eye, though pupal lethality is not increased.

      Coexpression of HsrωEP93D enhances the Scer\GAL4GMR.PF, Tak1Scer\UAS.cTa eye phenotype, though pupal lethality is not increased.

      (Mallik and Lakhotia, 2009)

      Co-expression of HsrωEP93D significantly enhances the Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1, Scer\GAL4GMR.PF eye phenotype, leading to extensive black lesions on the eye surface.

      Co-expression of a single copy of P{Sym-UAS-Hsrω} eliminates the enhancing effect of HsrωEP93D expression on the Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1, Scer\GAL4GMR.PF eye phenotype, and also substantially reverses the eye degeneration primarily induced by Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1.

      (Mallik and Lakhotia, 2009)

      The neurodegeneration phenotype caused by expression of Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4GMR.PF is enhanced by co-expression of HsrωEP93D under the control of Scer\GAL4GMR.PF; extensive black necrotic lesions are seen in the eye.

      (Sengupta and Lakhotia, 2006)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer

      FlyBase curator comment: the HsrωEP93D allele was generated from Hsrω05241, which has since been shown (see FBrf0205520) to carry a second site mutation (ms(3)2121) on the chromosome. Thus the HsrωEP93D chromosome may also carry this second site mutation.

      (Sengupta and Lakhotia, 2006)

      Separable from: A secondary mutation causing recessive male sterility.

      (Mallik and Lakhotia, 2009)

      The P{PZ} transposon of P{PZ}Hsrω05241 was swapped with an unspecified P{EP} transposon present in the robo gene to create P{EP}HsrωEP93D.

      (Mallik and Lakhotia, 2009)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      EP93D
      (Lo Piccolo and Yamaguchi, 2017, Choudhury et al., 2016, Mallik and Lakhotia, 2011, Mallik and Lakhotia, 2009, Sengupta and Lakhotia, 2006)
      HsrωEP93D
      lncRNA:HsrωEP93D
      Name Synonyms
      Secondary FlyBase IDs
        References (9)