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General Information
Symbol
Dmel\Tak1UAS.cTa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Takatsu
FlyBase ID
FBal0117635
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-DTak1, tak1, UAS-dTak
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

The full length Tak1 cDNA is expressed under the control of UASt sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Tak1Scer\UAS.cTa under the control of Scer\GAL4wor.PA induces neuroblast loss in larval brains.

Overexpression of Tak1Scer\UAS.cTa under the control of Scer\GAL4GMR.PF, induces autophagy in developing eye discs. This results in a lack of eye pigmentation and a rough, impaired appearance compared with control flies.

Scer\GAL4GMR.PF-driven expression of Tak1Scer\UAS.cTa results in a non-pigmented and small eye and lethality of 45% of differentiated pupae.

Expression of Tak1Scer\UAS.cTa under the control of Scer\GAL4GMR.PF results in a small eye phenotype.

When expression is driven by Scer\GAL4prd.RG1, anterior rows of denticles are lost in even numbered segments.

most Tak1Scer\UAS.cTa,Scer\GAL4GMR.PF animals die at pupal stages when grown at 25oC. They exhibit a small eye phenotype. The number of apoptotic cells increases dramatically in regions posterior to the morphogenetic furrow in the developing eye. Sequential recruitment of the photoreceptor cells is disrupted in mutant eye discs. Early photoreceptor induction appears to occur normally but later photoreceptor induction is delayed or disrupted and photoreceptor clustering shows an abnormal and diffuse pattern.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
NOT Enhanced by
Suppressed by
Statement
Reference
NOT suppressed by
Other
Phenotype Manifest In
Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

The number of autolysosomes, indicating the level of autophagy, is increased upon co-expression of S6kKQ.Scer\UAS and Tak1Scer\UAS.cTa in the developing eye disc, under the control of Scer\GAL4GMR.PF, compared to expression of either transgene alone.

The number of autolysosomes, indicating the level of autophagy, is decreased upon co-expression of S6kScer\UAS.cMa and Tak1Scer\UAS.cTa in the developing eye disc, under the control of Scer\GAL4GMR.PF, compared to expression of Tak1Scer\UAS.cTa alone.

Co-expression of P{Sym-UAS-Hsrω} suppresses the eye phenotype of Scer\GAL4GMR.PF, Tak1Scer\UAS.cTa, and also substantially reduces the mortality of differentiated pupae.

Coexpression of HsrωEP93D or HsrωEP3037 enhances the Scer\GAL4GMR.PF, Tak1Scer\UAS.cTa eye phenotype, though pupal lethality is not increased.

The reduced eye phenotype caused by expression of Rab11N124I.Scer\UAS under the control of Scer\GAL4GMR.PF is enhanced by co-expression of Tak1Scer\UAS.cTa.

Coexpression of Tak1Scer\UAS.cTa, with Traf1EP578, under the control of Scer\GAL4GMR.PF, an increased eye roughness and reduced eye size compared to expression of just Traf1EP578.

The addition of BacA\p35GMR.PH to Tak1Scer\UAS.cTa,Scer\GAL4GMR.PF flies suppresses the cell death phenotype in the eye, but has no effect on the abnormal photoreceptor clustering seen in the eye disc. The addition of hep1/Y to Tak1Scer\UAS.cTa,Scer\GAL4GMR.PF flies the reduced eye size phenotype is rescued almost to wild-type. The addition of bsk2/+ to Tak1Scer\UAS.cTa,Scer\GAL4GMR.PF flies the reduced eye size phenotype is rescued almost to wild-type.

Xenogenetic Interactions
Statement
Reference

Expression of BacA\p35Scer\UAS.cHa does not suppress the neuroblast loss seen in larval brains expressing Tak1Scer\UAS.cTa under the control of Scer\GAL4wor.PA.

Despite suppression of apoptosis through expression of BacA\p35Scer\UAS.cHa, a rough, impaired adult eye phenotype is found in BacA\p35Scer\UAS.cHa/Scer\GAL4GMR.PF; Tak1Scer\UAS.cTa flies, indicating that the Tak1-induced impaired eye phenotype is mainly due to autophagy.

Complementation and Rescue Data
Partially rescues
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
Tak1Scer\UAS.cTa
Tak1UAS.cTa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Takatsu
Secondary FlyBase IDs
    References (15)