The additional clonal co-expression of bskDN.Scer\UAS.cUa suppresses the cell non-autonomous increase in autophagosomes (assessed by a Atg8a fluorescence reporter) in the midgut, but not in the somatic muscles or the fat body, of third instar larvae resulting from the presence of eye-antennal imaginal disc clones homozygous for scrib673 and expressing Ras85DV12.Scer\UAS under the control of Scer\GAL4Act.PU; the clonal co-expression of bskDN.Scer\UAS.cUa also suppresses the cell non-autonomous increase in autophagosomes in neighboring disc cells resulting from third instar larval eye-antennal imaginal disc clones co-expressing dlg1GD4689 and Ras85DV12.Scer\UAS under the control of Scer\GAL4Act5C.CoinFLP-FRT (in combination with Dicer-2 for efficient RNAi).
Both the frequent follicular epithelium multi-layering and the occasional invasion of germline cells by follicle cells exhibited by Pi3K59FΔm22 homozygous follicle cell clones is strongly suppressed by the clonal expression of bskDN.Scer\UAS.cUa under the control of Scer\GAL4tub.PU.
The co-expression of bskDN.UAS.cUa suppresses both the increased numbers of intestinal stem cells/enteroblast and the increased mitotic index induced in the adult midgut epithelium by the adulthood-only expression of UvragHMS01357 under the control of Scer\GAL4esg-NP5130 (and Gal80[ts]).
Co-expression of bskDN.Scer\UAS.cUa with Fas2Scer\UAS.cLb (or Fas2Scer\UAS.PEST+.KNSAV.T:Zzzz\FLAG, Fas2Scer\UAS.PEST-.KNSAV.T:Zzzz\FLAG, Fas2Scer\UAS.CTshort.KNSAV.T:Zzzz\FLAG, Fas2Scer\UAS.ΔCT.KNSAV.T:Zzzz\FLAG, Fas1+3.Scer\UAS, Nrg180.Scer\UAS) by Scer\GAL4Tab2-201Y results in mushroom body γ lobe axon pruning defects.
Co-expression of bskDN.Scer\UAS.cUa with Fas2Scer\UAS.ΔCT.AAV.T:Zzzz\FLAG (or Fas2Scer\UAS.PEST-.AAV.T:Zzzz\FLAG or Fas3Scer\UAS.cZa) by Scer\GAL4Tab2-201Y does not result in mushroom body pruning defects.
Clonal co-expression of bskDN.Scer\UAS.cUa suppresses the invasiveness of clonal cells, but does not suppress tumor formation or developmental delay seen with eye-antennal disc clones co-expressing Ets21CKK103211 and Ras85DV12.Scer\UAS under the control of Scer\GAL4Act.PU.
The aggressive tissue overgrowth observed in third instar larval wing discs carrying somatic (flip-out) clones simultaneously expressing Snr1HMS00363 and BacA\p35Scer\UAS.cHa driven by Scer\GAL4Act.PU is significantly suppressed by co-expression of bskDN.Scer\UAS.cUa.