FlyBase Allele Report: Dmel\Bub3[GD9924]

General Information

Symbol

Dmel\Bub3^GD9924

Species

D. melanogaster

Name

FlyBase ID

FBal0198851

Feature type

allele

Associated gene

Dmel\Bub3

Associated Insertion(s)

Carried in Construct

P{GD9924}

Also Known As

bub3-i

Key Links

Genomic Maps

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Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

in vitro construct - RNAi

(Dickson et al., 2007.7.18)

in vitro construct - regulatory fusion

(Dickson et al., 2007.7.18)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{GD9924}

Cytology

Nature of the lesion

Statement

Reference

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Product class / Tool use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Bub3

Associated Sequence Features

dsRNA-GD9924

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Adults expressing Bub3^GD9924 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 60-70% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 20-30% of the Scer\GAL4^pnr-MD237 expression domain.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

9924

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

BacA\p35^UAS.cHa, Bub3^GD9924, Scer\GAL4^ap-md544 has neoplasia | larval stage phenotype, enhanceable by stg^UAS.cNa, Scer\GAL4^ap-md544

(Muzzopappa et al., 2017)

Suppressed by

Statement

Reference

BacA\p35^UAS.cHa, Bub3^GD9924, Scer\GAL4^ap-md544 has increased cell number | larval stage phenotype, suppressible by rpr^UAS.C/Scer\GAL4^He.PZ

(Muzzopappa et al., 2017)

BacA\p35^UAS.cHa, Bub3^GD9924, Scer\GAL4^ap-md544 has neoplasia | larval stage phenotype, suppressible by trbl^UAS.cMa, Scer\GAL4^ap-md544

(Muzzopappa et al., 2017)

NOT suppressed by

Statement

Reference

BacA\p35^UAS.cHa, Bub3^GD9924, Scer\GAL4^ap-md544 has neoplasia | larval stage phenotype, non-suppressible by rpr^UAS.C/Scer\GAL4^He.PZ

(Muzzopappa et al., 2017)

BacA\p35^UAS.cHa, Bub3^GD9924, Scer\GAL4^ap-md544 has neoplasia | larval stage phenotype, non-suppressible by rpr^lexAop.cSa/Ecol\lexA^GMR15B03

(Muzzopappa et al., 2017)

BacA\p35^UAS.cHa, Bub3^GD9924, Scer\GAL4^ap-md544 has increased cell number | larval stage phenotype, non-suppressible by rpr^lexAop.cSa/Ecol\lexA^GMR15B03

(Muzzopappa et al., 2017)

Other

Statement

Reference

BacA\p35^UAS.cHa, Bub3^GD9924, Scer\GAL4^ap-md544 has increased cell number | larval stage phenotype

(Muzzopappa et al., 2017)

BacA\p35^UAS.cHa, Bub3^GD9924, Scer\GAL4^ap-md544 has neoplasia | larval stage phenotype

(Muzzopappa et al., 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

BacA\p35^UAS.cHa, Bub3^GD9924, Scer\GAL4^ap-md544 has wing disc phenotype, enhanceable by stg^UAS.cNa, Scer\GAL4^ap-md544

(Muzzopappa et al., 2017)

Suppressed by

Statement

Reference

BacA\p35^UAS.cHa, Bub3^GD9924, Scer\GAL4^ap-md544 has embryonic/larval hemocyte | increased number | larval stage phenotype, suppressible by rpr^UAS.C/Scer\GAL4^He.PZ

(Muzzopappa et al., 2017)

BacA\p35^UAS.cHa, Bub3^GD9924, Scer\GAL4^ap-md544 has wing disc phenotype, suppressible by trbl^UAS.cMa, Scer\GAL4^ap-md544

(Muzzopappa et al., 2017)

NOT suppressed by

Statement

Reference

BacA\p35^UAS.cHa, Bub3^GD9924, Scer\GAL4^ap-md544 has wing disc phenotype, non-suppressible by rpr^UAS.C/Scer\GAL4^He.PZ

(Muzzopappa et al., 2017)

BacA\p35^UAS.cHa, Bub3^GD9924, Scer\GAL4^ap-md544 has wing disc phenotype, non-suppressible by rpr^lexAop.cSa/Ecol\lexA^GMR15B03

(Muzzopappa et al., 2017)

BacA\p35^UAS.cHa, Bub3^GD9924, Scer\GAL4^ap-md544 has myoblast | increased number | larval stage phenotype, non-suppressible by rpr^lexAop.cSa/Ecol\lexA^GMR15B03

(Muzzopappa et al., 2017)

Other

Statement

Reference

BacA\p35^UAS.cHa, Bub3^GD9924, Scer\GAL4^ap-md544 has myoblast | increased number | larval stage phenotype

(Muzzopappa et al., 2017)

BacA\p35^UAS.cHa, Bub3^GD9924, Scer\GAL4^ap-md544 has wing disc phenotype

(Muzzopappa et al., 2017)

BacA\p35^UAS.cHa, Bub3^GD9924, Scer\GAL4^ap-md544 has embryonic/larval hemocyte | increased number | larval stage phenotype

(Muzzopappa et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The co-expression of Bub3^GD9924 and BacA\p35^UAS.cHa under the control of Scer\GAL4^ap-md544 induces wing disc neoplasia which, when transplanted, can form solid tumors in the host; in both wing discs and allografts there is amplification of the resident myoblast population, as well as accumulation of circulating hemocytes. The same tumor induction and hemocyte recruitment occurs if expression is only induced in wing disc transplants (expression induction is controlled by Gal80[ts] and temperature-shift); hemocyte ablation on the host (by expressing rpr^UAS.C under the control of Scer\GAL4^He.PZ) does not suppress the tumor-like growth; myoblast ablation on the host (by expressing rpr^lexAop.cSa under the control of Ecol\lexA^GMR15B03) does not suppress the tumor-like growth.