Expression of p53GD4483 under the control of any of three fat body drivers (Scer\GAL4ppl.PP, Scer\GAL4cg.PU or Scer\GAL4yolk) results in reduced survival rates upon adult nutrient deprivation when compared to controls. Expressing p53GD4483 in muscles under the control of Scer\GAL4Mef2.PR has no effect on survival rates upon fasting.
Fat-body specific expression of p53GD4483 under the control of Scer\GAL4ppl.PP causes an accelerated consumption of energy resources following fasting; triacylglycerides (TAGs) and glycogen are consumed at an accelerated rate compared to controls and the levels of total sugar are also reduced. Levels of circulating sugars are unaffected. The levels of TAGS, glycogen and sugars are largely similar in p53-depleted and control animals prior to starvation. The lipid droplets in fat body cells are smaller than in starved control flies. No significant change in lipid droplet size is observed in well-fed fat body cells expressing p53GD4483.
Expression of p53GD4483 under the control of Scer\GAL4ple.PF does not lead to a significant difference in survival or climbing capability as compared to controls under normal conditions; however, these flies exhibit increased resistance to paraquat toxicity, with a significant increase in survival and climbing performance when exposed to paraquat, as compared to control flies exposed to the same treatment.
Adults expressing p53GD4483 under the control of Scer\GAL4elav.PLu (in the presence of Dcr-2Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.