Please see the JBrowse view of Dmel\Bub3 for information on other features
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Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.49
327 (aa); 37 (kD observed); 37 (kD predicted)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Bub3 using the Feature Mapper tool.
Comment: maternally deposited
Comment: Localization observed prometaphase until late anaphase of embryonic cycle 14
Subcellular localization to kinetochores of embryonic mitotic cells assayed using the transgenic allele Bub3T:Avic\GFP-EGFP.
Comment: Localization observed prometaphase until late anaphase of embryonic cycle 14
GBrowse - Visual display of RNA-Seq signals
View Dmel\Bub3 in GBrowse 23-99
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
FlyBase curator comment: the insertion in the "f03381" Exelixis line (PBac{WH}CAP-D2f03381) was originally assigned to the Bub3 gene in FBrf0179797, resulting in the "Bub3f03381" (FBal0162743) allele. However, FBrf0184340 shows that the insertion is actually within CAP-D2.
RNAi screen using dsRNA made from templates generated with primers directed against this gene results in chromosome misalignment on the metaphase spindle when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.