FB2021_01, released Feb 18, 2021

Gene: Dmel\Bub3

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General Information
Symbol
Dmel\Bub3
Species
D. melanogaster
Name
Bub3
Annotation Symbol
CG7581
Feature Type
protein_coding_gene
FlyBase ID
FBgn0025457
Gene Model Status
Current
Stock Availability
8 publicly available
Gene Snapshot
Bub3 (Bub3) encodes a protein that functions in the spindle assembly checkpoint (SAC) pathway. This mitotic checkpoint pathway inhibits the APC/C complex and hence the transition from metaphase to anaphase, as long as there are free kinetochores that are not yet attached to the mitotic spindle in the cell. The product of Bub3 is localized at the kinetochore during mitosis with maximal levels observed at free kinetochores. Together with other SAC proteins it forms mitotic checkpoint complexes that bind and inhibit the APC/C. [Date last reviewed: 2019-03-07]
Other Summaries
Key Links
Genomic Location
Cytogenetic map
99B7-99B7
Sequence location
3R:29,674,007..29,675,421 [-]
Recombination map

3-99

RefSeq locus
NT_033777 REGION:29674007..29675421
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (7 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
enables ubiquitin binding
inferred from biological aspect of ancestor with PANTHER:PTN000103837
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
involved_in mitotic spindle assembly checkpoint
inferred from direct assay
(Logarinho et al., 2004)
involved_in regulation of chromosome separation
inferred from mutant phenotype
(Clemente-Ruiz et al., 2016)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in mitotic spindle assembly checkpoint
inferred from biological aspect of ancestor with PANTHER:PTN000103865
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (4 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
located_in kinetochore
inferred from direct assay
(Schittenhelm et al., 2007, Logarinho et al., 2004, Basu et al., 1998)
located_in nucleoplasm
inferred from direct assay
(Basu et al., 1998)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
part_of bub1-bub3 complex
inferred from biological aspect of ancestor with PANTHER:PTN000103865
(assigned by GO_Central )
(Gaudet et al., 2011)
located_in kinetochore
inferred from biological aspect of ancestor with PANTHER:PTN000103865
(assigned by GO_Central )
(Gaudet et al., 2011)
part_of mitotic checkpoint complex
inferred from biological aspect of ancestor with PANTHER:PTN000103865
(assigned by GO_Central )
(Gaudet et al., 2011)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Summary (Interactive Fly)
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Bub3 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.49

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Bub3-RA
FBtr0085465
NM_058033
1236
326
Bub3-RB
FBtr0339148
NM_001300658
1362
326
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
Bub3-PA
FBpp0084831
37.4
326
6.51
NP_477381
AAF56914
Bub3-PB
FBpp0308293
37.4
326
6.51
NP_001287587
AHN57586
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

326 aa isoforms: Bub3-PA, Bub3-PB
Additional Polypeptide Data and Comments
Reported size (kDa)

327 (aa); 37 (kD observed); 37 (kD predicted)

(Basu et al., 1998)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Bub3 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Polypeptide Expression
No Assay Recorded
Stage
Tissue/Position (including subcellular localization)
Reference
larval stage
neuroblast
(Basu et al., 1998)
adult stage | male
spermatocyte
(Basu et al., 1998)
epitope tag
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 4 -- 5
cell

Comment: Localization observed prometaphase until late anaphase of embryonic cycle 14

(Schittenhelm et al., 2007)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data

Subcellular localization to kinetochores of embryonic mitotic cells assayed using the transgenic allele Bub3T:Avic\GFP-EGFP.

(Schittenhelm et al., 2007, Basu et al., 1998)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in kinetochore
inferred from direct assay
(Schittenhelm et al., 2007, Logarinho et al., 2004, Basu et al., 1998)
located_in nucleoplasm
inferred from direct assay
(Basu et al., 1998)
Expression Deduced from Reporters
Reporter: P{gEGFP-Bub3}
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 4 -- 5
cell

Comment: Localization observed prometaphase until late anaphase of embryonic cycle 14

(Schittenhelm et al., 2007)
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Bub3 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 11-12
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 2 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 8 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Bub3
Transgenic constructs containing regulatory region of Bub3
Aberrations (Deficiencies and Duplications) ( 0 )
Inferred from experimentation
Inferred from location
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
Other Phenotypes
Allele
visible, with Scer\GAL4pnr-MD237
Phenotype manifest in
Allele
trichogen cell, with Scer\GAL4pnr-MD237
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\BUB3
13 of 15
Yes
Yes
Hsap\RAE1
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Bub3
13 of 15
Yes
Yes
Rattus norvegicus (Norway rat) (2)
Rnor\Bub3
6 of 13
Yes
Yes
Rnor\Rae1
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (2)
Xtro\bub3
7 of 12
Yes
Yes
Xtro\rae1
1 of 12
No
No
Danio rerio (Zebrafish) (3)
Drer\bub3
13 of 15
Yes
Yes
Drer\LOC103910025
1 of 15
No
Yes
Drer\rae1
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (2)
Cele\bub-3
12 of 15
Yes
Yes
Cele\rae-1
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (5)
Atha\BUB3.1
8 of 9
Yes
Yes
Atha\BUB3.2
8 of 9
Yes
Yes
Atha\AT1G69400
2 of 9
No
Yes
Atha\AT1G15850
1 of 9
No
No
Atha\RAE1
1 of 9
No
No
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\BUB3
5 of 15
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\bub3
10 of 12
Yes
Yes
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190ARD )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00011185
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00012188
Drosophila simulans
Dsim\GD17697
Drosophila sechellia
Dsec\GM12232
Drosophila erecta
Dere\GG26418
Drosophila yakuba
Dyak\GE10441
Drosophila ananassae
Dana\GF22876
Drosophila pseudoobscura pseudoobscura
Dpse\GA20454
Drosophila persimilis
Dper\GL13583
Drosophila willistoni
Dwil\GK11174
Drosophila virilis
Dvir\GJ10544
Drosophila mojavensis
Dmoj\GI24196
Drosophila grimshawi
Dgri\GH18686
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091506NM )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17011223
Glossina morsitans
Tsetse fly
Gmor\GMOY001233
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_09885
Mayetiola destructor
Hessian fly
Mdes\Mdes004302
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL007469
Anopheles darlingi
American malaria mosquito
Adar\ADAC005826
Anopheles darlingi
American malaria mosquito
Adar\ADAC005907
Anopheles gambiae
Malaria mosquito
Agam\AGAP010544
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ016504
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W06UK )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA004646
Danaus plexippus
Monarch butterfly
Dple\DPOGS212380-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL003943
Apis florea
Little honeybee
Aflo\2758868500
Apis mellifera
Western honey bee
Amel\Bub3
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP25584
Bombus terrestris
Buff-tailed bumblebee
Bter\15207771
Linepithema humile
Argentine ant
Lhum\LH11994
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:0020da
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG000469
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_00494
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_07086
Tribolium castaneum
Red flour beetle
Tcas\Bub3
Pediculus humanus
Human body louse
Phum\PHUM515720
Rhodnius prolixus
Kissing bug
Rpro\RPRC008211
Cimex lectularius
Bed bug
Clec\CLEC007913
Cimex lectularius
Bed bug
Clec\CLEC001925
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI009048
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_01108
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X06PT )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR000759
Ixodes scapularis
Black-legged tick
Isca\ISCW000999
Stegodyphus mimosarum
African social velvet spider
Smim\KFM75540.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur11g03390
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_310020
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0AB8 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Bub3h
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000009630
Gallus gallus
Domestic chicken
Ggal\BUB3
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (3)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
CG12782
3 of 10
Rae1
3 of 10
Wdr92
2 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Allele
Disease
Evidence
References
Potential Models Based on Orthology ( 0 )
Human Ortholog
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Allele
Disease
Interaction
References
Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
BUB3; BUB3 mitotic checkpoint protein
13 of 15
603719
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      esyN Network Diagram
      Show neighbor-neighbor interactions:
      Select Layout:
      Legend:
      Protein
      RNA
      Selected Interactor(s)
      Interactions Browser
      View in Interactions Browser
      Please see the Physical Interaction reports below for full details
      protein-protein
      Physical Interaction
      Assay
      References
      Bub3 - Cenp-C
      anti tag coimmunoprecipitation, peptide massfingerprinting
      (Przewloka et al., 2011)
      Bub3 - ft
      anti tag coimmunoprecipitation, peptide massfingerprinting
      (Kwon et al., 2013)
      Summary of Genetic Interactions
      esyN Network Diagram
      esyN Network Key:
      Suppression
      Enhancement
      View in Interactions Browser
      Please look at the allele data for full details of the genetic interactions
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Bub3
      suppressible
      rpr
      (Muzzopappa et al., 2017)
      Bub3
      enhanceable
      stg
      (Muzzopappa et al., 2017)
      Bub3
      suppressible
      trbl
      (Muzzopappa et al., 2017)
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      External Data
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Pathways
      Signaling Pathways (FlyBase)
      Metabolic Pathways
      External Data
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      3R
      Recombination map

      3-99

      Cytogenetic map
      99B7-99B7
      Sequence location
      3R:29,674,007..29,675,421 [-]
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      99B7-99B7
      Limits computationally determined from genome sequence between P{PZ}l(3)0674306743 and P{PZ}ncd05884
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      22F-23A
      (Delgrossi, 1998.11.13)
      99B2-99B4
      (Basu, 1998.8.28, Basu et al., 1998)
      99B3-99B5
      (determined by in situ hybridisation)
      (Andre, 1999.4.14)
      Map position is based on hybridisation of a Bub3 cDNA clone to P1 clones DS04127 and DS07294.
      (Basu et al., 1998)
      Experimentally Determined Recombination Data
      Location

      3-99

      (FlyBase, 2016)

      3-96.7

      (Comeron et al., 2012)

      3-

      (Horn et al., 2003)
      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (8)
      Genomic Clones (9)
       

      Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

      cDNA Clones (106)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequenced
      BDGP DGC clones
      Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
      Other clones
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Antibody Information
      Laboratory Generated Antibodies
       

      polyclonal

      (Morais da Silva et al., 2013, Logarinho et al., 2004, Basu et al., 1998)
      Commercially Available Antibodies
       
      Other Information
      Relationship to Other Genes
      Source for database identify of
      Source for database merge of
      Additional comments

      FlyBase curator comment: the insertion in the "f03381" Exelixis line (PBac{WH}CAP-D2f03381) was originally assigned to the Bub3 gene in FBrf0179797, resulting in the "Bub3f03381" (FBal0162743) allele. However, FBrf0184340 shows that the insertion is actually within CAP-D2.

      (Bloomington Drosophila Stock Center, 2004.11.6)
      Other Comments

      RNAi screen using dsRNA made from templates generated with primers directed against this gene results in chromosome misalignment on the metaphase spindle when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

      (Goshima et al., 2007)

      dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

      (Kiger et al., 2003)
      Origin and Etymology
      Discoverer
      Etymology
      Identification
      External Crossreferences and Linkouts ( 54 )
      Sequence Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
      Other crossreferences
      BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Flygut - An atlas of the Drosophila adult midgut
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
      InterPro - A database of protein families, domains and functional sites
      KEGG Genes - Molecular building blocks of life in the genomic space.
      modMine - A data warehouse for the modENCODE project
      SignaLink - A signaling pathway resource with multi-layered regulatory networks.
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DPiM - Drosophila Protein interaction map
      DroID - A comprehensive database of gene and protein interactions.
      DRSC - Results frm RNAi screens
      FLIGHT - Cell culture data for RNAi and other high-throughput technologies
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
      FlyMine - An integrated database for Drosophila genomics
      Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      MIST (protein-protein) - An integrated Molecular Interaction Database
      Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
      Synonyms and Secondary IDs (6)
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (84)