FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000121599
Interaction Network
Interactions Browser links
Spc105R network
Bub3 network
Reported Interactions
FBrf0252105-1.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Spc105

bait

Streptavadin-TST; 6His tags

Bub3 

Bub3

prey

EGFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
10X MEED Motif region
sufficient binding region
aa 704-1138
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

interaction increased 30 fold upon phosphorylation of Spc105R by addition of ATP and activated Ipl1 budding yeast kinase.

External Crossreferences and Linkouts ( 0 )
References (1)