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General Information
Symbol
Dmel\p53H159N.UAS.Ex
Species
D. melanogaster
Name
FlyBase ID
FBal0117416
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Dp53H159N, UAS-p53H159N
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Expression of p53 open reading frame is driven by the UAS regulatory sequences. The alpha tubulin 3' UTR provides stability to the message. Amino acid replacement: H159N.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
DOES NOT model  cancer
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of p53H159N.Scer\UAS.Ex under the control of any of three fat body drivers (Scer\GAL4ppl.PP, Scer\GAL4cg.PU or Scer\GAL4yolk) results in reduced survival rates upon adult nutrient deprivation when compared to controls. Expressing p53H159N.Scer\UAS.Ex in muscles under the control of Scer\GAL4Mef2.PR has no effect on survival rates upon fasting.

Fat-body specific expression of p53H159N.Scer\UAS.Ex under the control of Scer\GAL4ppl.PP causes an accelerated consumption of energy resources following fasting; triacylglycerides (TAGs) and glycogen are consumed at an accelerated rate compared to controls and the levels of total sugar are also reduced. Levels of circulating sugars are unaffected. The levels of TAGS, glycogen and sugars are largely similar in p53-depleted and control animals prior to starvation.

Expression of p53H159N.Scer\UAS.Ex under the control of Scer\GAL4insc-Mz1407 has no effect on neuroblast number.

The expression of p53H159N.Scer\UAS.Ex under the control of Scer\GAL4en-e16E has little effect on viability.

When expression is driven in the posterior compartment of the wing disc by Scer\GAL4en-e16E, X irradiation-induced apoptosis is blocked. Wild type discs show cell cycle block upon X-irradiation - this is unaffected by p53H159N.Scer\UAS.Ex.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Suppressor of
NOT Suppressor of
Other
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The co-expression of p53H159N.UAS.Ex does not suppress the apoptosis in the third instar larval wing disc dorsal compartment induced by the expression of pnutGD1512 from the second instar larval stage under the combined control of Scer\GAL4ap-md544 and Gal80[ts]; this co-expression does not lead to wing disc tumors.

The extensive cell death in the pouch region of wing discs in third instar larvae as well as the small and deformed wings in adults expressing His1:CG31617NIG.31617R under the control of Scer\GAL4nub.PU are further worsened by co-expression of p53H159N.Scer\UAS.Ex.

The shortening of the dendritic arbor in class IV ddaC neurons in third instar larvae expressing prelScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4109(2)80 is not significantly affected by co-expression of p53H159N.Scer\UAS.Ex.

Expression of p53H159N.Scer\UAS.Ex suppresses the increase in survival rate in response to starvation in adult flies expressing Dcr-1GD11429 in the fat body under the control of Scer\GAL4ppl.PP.

Co-expression of p53H159N.Scer\UAS.Ex (that lacks transactivation activity) does not suppress supernumerary neuroblast formation induced by numbTS4D.Scer\UAS expression under the control of Scer\GAL4insc-Mz1407.

The lethality of animals expressing Fcp1dsRNA.Scer\UAS.WIZ under the control of Scer\GAL4en-e16E is shifted to an earlier phase if the animals are also co-expressing p53H159N.Scer\UAS.Ex.

Co-expression of p53H159N.Scer\UAS.Ex and Fcp1Scer\UAS.cTa under the control of Scer\GAL4vg.PU results in a nicked wings phenotype (this phenotype is not seen when either transgene is expressed alone).

Xenogenetic Interactions
Statement
Reference

The additional co-expression of p53H159N.Scer\UAS.Ex does not modify the fully penetrant rough eye phenotype resulting from the co-expression of HPV18\E6Scer\UAS.T:Hsap\MYC and Hsap\UBE3AScer\UAS.cRa under the control of Scer\GAL4GMR.PU.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (3)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
p53H159N.Scer\UAS.Ex
p53H159N.UAS.Ex
Name Synonyms
Secondary FlyBase IDs
    References (15)