FlyBase Allele Report: Dmel\pnut[GD1512]

General Information

Symbol

Dmel\pnut^GD1512

Species

D. melanogaster

Name

FlyBase ID

FBal0210369

Feature type

allele

Associated gene

Dmel\pnut

Associated Insertion(s)

Carried in Construct

P{GD1512}

Also Known As

UAS-pnut-RNAi, UAS-PNUT^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD1512}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD1512

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cancer

CEA with Ras85D^V12.UAS

(Gerlach et al., 2018)

CEA with wts^KK101055

(Gerlach et al., 2018)

CEA with yki^UAS.cHa

(Gerlach et al., 2018)

DOES NOT model cancer

CEA with p53^H159N.UAS.Ex

(Gerlach et al., 2018)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle | third instar larval stage, with Scer\GAL4^ap-md544

(Gerlach et al., 2018)

abnormal neurophysiology | adult stage, with Scer\GAL4^ninaE.PT

(Kumari et al., 2022)

abnormal size | third instar larval stage, with Scer\GAL4^ap-md544

(Gerlach et al., 2018)

abnormal size | third instar larval stage, with Scer\GAL4^e22c

(Gerlach et al., 2018)

abnormal size | third instar larval stage, with Scer\GAL4^hh.PU

(Gerlach et al., 2018)

increased cell death | third instar larval stage, with Scer\GAL4^ap-md544

(Gerlach et al., 2018)

partially lethal - majority die, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

Detailed Description

Statement

Reference

Adults expressing pnut^GD1512 under the control of Scer\GAL4^GMR.PF or Scer\GAL4^ninaE.PT show increased retinal electrical response to green light (increased amplitude), compared to controls.

(Kumari et al., 2022)

The expression of pnut^GD1512 from the second instar larval stage under the combined control of Scer\GAL4^ap-md544 and Gal80[ts] leads to the third instar larval wing disc dorsal compartment presenting a significant decrease in size, associated with polyploidy/aneuploidy, an increase in apoptosis (assessed by activated Caspase3 immunofluorescence) and an increase in the average number of centrosomes per cell, as compared to controls. Expression under the control of either Scer\GAL4^hh.PU or Scer\GAL4^e22c leads to a smaller third instar larval wing disc posterior compartment, as compared to controls.

(Gerlach et al., 2018)

Adults expressing pnut^GD1512 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 10-20% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 10-20% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in planar polarity defects in the orientation of bristles of the notum in 20-30% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 40-50% of the Scer\GAL4^pnr-MD237-expressing area of the notum.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1512

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^ap-md544, pnut^GD1512 has abnormal mitotic cell cycle | third instar larval stage phenotype, enhanceable by Diap1^UAS.cUa, Scer\GAL4^ap-md544

(Gerlach et al., 2018)

Scer\GAL4^ap-md544, pnut^GD1512 has abnormal mitotic cell cycle | third instar larval stage phenotype, enhanceable by yki^UAS.cHa/Scer\GAL4^ap-md544

(Gerlach et al., 2018)

Scer\GAL4^ap-md544, pnut^GD1512, yki^UAS.cHa has neoplasia | third instar larval stage phenotype, enhanceable by bsk^DN.UAS/Scer\GAL4^ap-md544

(Gerlach et al., 2018)

Scer\GAL4^ap-md544, pnut^GD1512 has abnormal mitotic cell cycle | third instar larval stage phenotype, enhanceable by BacA\p35^UAS.cHa, Scer\GAL4^ap-md544

(Gerlach et al., 2018)

Suppressed by

Statement

Reference

Scer\GAL4^ap-md544, pnut^GD1512 has increased cell death | third instar larval stage phenotype, suppressible | partially by bsk^DN.UAS/Scer\GAL4^ap-md544

(Gerlach et al., 2018)

Scer\GAL4^ap-md544, pnut^GD1512 has abnormal size | third instar larval stage phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^ap-md544

(Gerlach et al., 2018)

Scer\GAL4^ap-md544, pnut^GD1512 has abnormal size | third instar larval stage phenotype, suppressible by Diap1^UAS.cUa, Scer\GAL4^ap-md544

(Gerlach et al., 2018)

Scer\GAL4^ap-md544, pnut^GD1512 has increased cell death | third instar larval stage phenotype, suppressible by Diap1^UAS.cUa, Scer\GAL4^ap-md544

(Gerlach et al., 2018)

NOT suppressed by

Statement

Reference

Scer\GAL4^ap-md544, pnut^GD1512 has increased cell death | third instar larval stage phenotype, non-suppressible by p53^5A-1-4/p53^5A-1-4

(Gerlach et al., 2018)

Scer\GAL4^ap-md544, pnut^GD1512 has increased cell death | third instar larval stage phenotype, non-suppressible by p53^H159N.UAS.Ex/Scer\GAL4^ap-md544

(Gerlach et al., 2018)

Enhancer of

Statement

Reference

Scer\GAL4^hh.PU/pnut^GD1512 is an enhancer of abnormal size | third instar larval stage phenotype of Scer\GAL4^hh.PU, yki^UAS.cHa

(Gerlach et al., 2018)

Scer\GAL4^e22c/pnut^GD1512 is an enhancer of abnormal size | third instar larval stage phenotype of Scer\GAL4^hh.PU, yki^UAS.cHa

(Gerlach et al., 2018)

Scer\GAL4^ap-md544/pnut^GD1512 is an enhancer of abnormal size | third instar larval stage phenotype of Scer\GAL4^ap-md544, yki^UAS.cHa

(Gerlach et al., 2018)

NOT Enhancer of

Statement

Reference

Scer\GAL4^da.PU/pnut^GD1512, Scer\GAL80^ts.αTub84B is a non-enhancer of lethal - all die during pupal stage | heat sensitive phenotype of Scer\GAL4^da.PU, Scer\GAL80^ts.αTub84B, fzr^RNAi.UAS.WIZ

(Neuert et al., 2017)

NOT Suppressor of

Statement

Reference

Scer\GAL4^da.PU/pnut^GD1512, Scer\GAL80^ts.αTub84B is a non-suppressor of lethal - all die during pupal stage | heat sensitive phenotype of Scer\GAL4^da.PU, Scer\GAL80^ts.αTub84B, fzr^RNAi.UAS.WIZ

(Neuert et al., 2017)

Other

Statement

Reference

Scer\GAL4^ap-md544, pnut^GD1512, wts^KK101055 has neoplasia | third instar larval stage phenotype

(Gerlach et al., 2018)

Scer\GAL4^ap-md544, pnut^GD1512, yki^UAS.cHa has abnormal developmental rate phenotype

(Gerlach et al., 2018)

Scer\GAL4^ap-md544, pnut^GD1512, yki^UAS.cHa has increased body size | third instar larval stage phenotype

(Gerlach et al., 2018)

Scer\GAL4^ap-md544, pnut^GD1512, yki^UAS.cHa has abnormal cell polarity | third instar larval stage phenotype

(Gerlach et al., 2018)

Scer\GAL4^hh.PU, pnut^GD1512, yki^UAS.cHa has abnormal cell adhesion | third instar larval stage phenotype

(Gerlach et al., 2018)

Scer\GAL4^ap-md544, pnut^GD1512, yki^UAS.cHa has neoplasia | third instar larval stage phenotype

(Gerlach et al., 2018)

Ras85D^V12.UAS, Scer\GAL4^ap-md544, pnut^GD1512 has neoplasia | third instar larval stage phenotype

(Gerlach et al., 2018)

Diap1^UAS.cUa, Scer\GAL4^ap-md544, pnut^GD1512, stg^UAS.Tag:HA has abnormal size | third instar larval stage phenotype

(Gerlach et al., 2018)

BacA\p35^UAS.cHa, Scer\GAL4^ap-md544, pnut^GD1512 has decreased occurrence of cell division | third instar larval stage phenotype

(Gerlach et al., 2018)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^ap-md544, pnut^GD1512 has wing disc dorsal compartment | third instar larval stage phenotype, enhanceable by Diap1^UAS.cUa, Scer\GAL4^ap-md544

(Gerlach et al., 2018)

Scer\GAL4^ap-md544, pnut^GD1512 has wing disc dorsal compartment | third instar larval stage phenotype, enhanceable by yki^UAS.cHa/Scer\GAL4^ap-md544

(Gerlach et al., 2018)

Scer\GAL4^ap-md544, pnut^GD1512, yki^UAS.cHa has wing disc | third instar larval stage phenotype, enhanceable by bsk^DN.UAS/Scer\GAL4^ap-md544

(Gerlach et al., 2018)

Scer\GAL4^ap-md544, pnut^GD1512 has wing disc dorsal compartment | third instar larval stage phenotype, enhanceable by BacA\p35^UAS.cHa, Scer\GAL4^ap-md544

(Gerlach et al., 2018)

Suppressed by

Statement

Reference

Scer\GAL4^ap-md544, pnut^GD1512 has wing disc dorsal compartment | third instar larval stage phenotype, suppressible by Diap1^UAS.cUa, Scer\GAL4^ap-md544

(Gerlach et al., 2018)

Scer\GAL4^ap-md544, pnut^GD1512 has wing disc dorsal compartment | third instar larval stage phenotype, suppressible | partially by bsk^DN.UAS/Scer\GAL4^ap-md544

(Gerlach et al., 2018)

Scer\GAL4^ap-md544, pnut^GD1512 has wing disc dorsal compartment | third instar larval stage phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^ap-md544

(Gerlach et al., 2018)

NOT suppressed by

Statement

Reference

Scer\GAL4^ap-md544, pnut^GD1512 has wing disc dorsal compartment | third instar larval stage phenotype, non-suppressible by p53^5A-1-4/p53^5A-1-4

(Gerlach et al., 2018)

Scer\GAL4^ap-md544, pnut^GD1512 has wing disc dorsal compartment | third instar larval stage phenotype, non-suppressible by p53^H159N.UAS.Ex/Scer\GAL4^ap-md544

(Gerlach et al., 2018)

Enhancer of

Statement

Reference

Scer\GAL4^hh.PU/pnut^GD1512 is an enhancer of wing disc posterior compartment | third instar larval stage phenotype of Scer\GAL4^hh.PU, yki^UAS.cHa

(Gerlach et al., 2018)

Scer\GAL4^e22c/pnut^GD1512 is an enhancer of wing disc posterior compartment | third instar larval stage phenotype of Scer\GAL4^hh.PU, yki^UAS.cHa

(Gerlach et al., 2018)

Scer\GAL4^ap-md544/pnut^GD1512 is an enhancer of wing disc dorsal compartment | third instar larval stage phenotype of Scer\GAL4^ap-md544, yki^UAS.cHa

(Gerlach et al., 2018)

Other

Statement

Reference

Scer\GAL4^ap-md544, pnut^GD1512, wts^KK101055 has wing disc | third instar larval stage phenotype

(Gerlach et al., 2018)

Scer\GAL4^ap-md544, pnut^GD1512, yki^UAS.cHa has wing disc dorsal compartment | third instar larval stage phenotype

(Gerlach et al., 2018)

Scer\GAL4^hh.PU, pnut^GD1512, yki^UAS.cHa has wing disc | third instar larval stage phenotype

(Gerlach et al., 2018)

Scer\GAL4^ap-md544, pnut^GD1512, yki^UAS.cHa has wing disc | third instar larval stage phenotype

(Gerlach et al., 2018)

Ras85D^V12.UAS, Scer\GAL4^ap-md544, pnut^GD1512 has wing disc | third instar larval stage phenotype

(Gerlach et al., 2018)

Diap1^UAS.cUa, Scer\GAL4^ap-md544, pnut^GD1512, stg^UAS.Tag:HA has wing disc dorsal compartment | third instar larval stage phenotype

(Gerlach et al., 2018)

BacA\p35^UAS.cHa, Scer\GAL4^ap-md544, pnut^GD1512 has wing disc dorsal compartment | third instar larval stage phenotype

(Gerlach et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

The third instar larval wing disc dorsal compartment smaller size and apoptosis induced by the expression of pnut^GD1512 from the second instar larval stage under the combined control of Scer\GAL4^ap-md544 and Gal80[ts] are suppressed by the co-expression of Diap1^UAS.cUa. pnut^GD1512's apoptosis phenotype is not suppressed by p53^5A-1-4 homozygosity or by the co-expression of p53^H159N.UAS.Ex; the pnut^GD1512, p53^H159N.UAS.Ex co-expression does not lead to the formation of wing disc tumors. The expression of pnut^GD1512 also leads induces polyploidy/aneuploidy phenotype in the wing disc dorsal compartment, which is enhanced by the co-expression of either Diap1^UAS.cUa or yki^UAS.cHa.

The co-expression of pnut^GD1512 and yki^UAS.cHa under the control of either Scer\GAL4^hh.PU or Scer\GAL4^e22c leads to a much more severe enlargement of the wing disc posterior compartment as compared to the yki^UAS.cHa single expression condition; the Scer\GAL4^hh.PU-driven co-expression also leads to some of the expressing cells moving and invading the wing disc anterior compartment.

The co-expression of pnut^GD1512 and yki^UAS.cHa from the second instar larval stage under the combined control of Scer\GAL4^ap-md544 and Gal80[ts] enhances the third instar larval wing disc dorsal compartment enlargement induced by the expression of yki^UAS.cHa from the second instar larval stage under the combined control of Scer\GAL4^ap-md544 and Gal80[ts], even leading to the formation of tumors and to cell polarity defects (i.e. mislocalization of Discs large protein); this tumor phenotype is enhanced by the co-expression of bsk^DN.UAS. The pnut^GD1512, yki^UAS.cHa co-expressing larvae fail to pupate and, instead, continue to grow to form giant larvae.

The expression of pnut^GD1512 in combination with wts^KK101055, Ras85D^V12.UAS, but not with scrib^HMS01993, dlg1^HMS01954, l(2)gl^HMS01905, Syx7^JF02436, Rab5^GD10492, TSG101^GD14295, Pten^KK109278, Tsc1^HMJ21477, Csk^HMS02277, ago^HMS00111, Egfr^UAS.cBa, Dl::N^ΔECN.UAS, dpp^UAS.cCa or wg^UAS.cGa, from the second instar larval stage under the combined control of Scer\GAL4^ap-md544 and Gal80[ts] also leads to the formation of tumors in the third instar larval wing disc.

The triple co-expression of pnut^GD1512, Diap1^UAS.cUa and stg^UAS.Tag:HA from the second instar larval stage under the combined control of Scer\GAL4^ap-md544 and Gal80[ts] leads to the significant enlargement of the third instar larval wing disc dorsal compartment, as compared to the pnut^GD1512, Diap1^UAS.cUa double expression condition.

(Gerlach et al., 2018)

Xenogenetic Interactions

Statement

Reference

The smaller third instar larval wing disc dorsal compartment and associated polyploidy/aneuploidy induced by the expression of pnut^GD1512 from the second instar larval stage under the combined control of Scer\GAL4^ap-md544 and Gal80[ts] are respectively suppressed and enhanced by the co-expression of BacA\p35^UAS.cHa; this pnut^GD1512, BacA\p35^UAS.cHa co-expression leads to a severe decrease in the number of cells undergoing DNA replication (assessed by EdU labelling and phospho-H3 immunostaining), as compared to controls.

(Gerlach et al., 2018)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v11791

w¹¹¹⁸ P{GD1512}v11791

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

1512

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

pnut^GD1512

pnut^RNAi

(di Pietro et al., 2023)

Name Synonyms

Secondary FlyBase IDs

References (16)

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