Gene model reviewed during 5.49
539 (aa); 60 (kD observed); 60 (kD predicted)
Ubiquitinated by park, leading to its degradation by the proteasome.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\pnut using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\pnut in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
dsRNA made from templates generated with primers directed against pnut that is transfected into S2 treated with Listeria monocytogenes reveals pnut to be involved in Listeria monocytogenes intracellular growth, with less intracellular growth observed in pnut-treated cells. pnut also seems to be involved in L.monocytogenes entry.
dsRNA directed against this gene causes defects in cytokinesis when tested in an RNAi screen in S2 cells.
A complex of septin polypeptides is isolated that bind and hydrolyse GTP and form filaments. Their high degree of conservation, ubiquitous expression and proven role in cytokinesis suggests septins are certain to be important players in regulating cell architecture and function. Septins alone can form regular filamentous polymers, since filament formation is likely to be central to their localisation and function.
pnut gene product may be required for normal sina activity. Mutations at pnut affect photoreceptor development and cell proliferation or survival as FLP/FRT mediated clones were smaller than the wild type twin spots.