FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Septin1, pnut
FlyBase ID
FBig0000054944
Interaction Network
Interactions Browser links
Septin1 network
pnut network
Reported Interactions
FBrf0085016-1.coIP.WB
Reference
Fares et al., 1995
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 16blastoderm stagegastrula stageembryonic stage 8dorsal closure stageembryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3extended germ band stageembryonic stage 4embryonic cycle 9embryonic stage 2late extended germ band stageembryonic stage 5embryonic stage 11embryonic stage 3embryonic stage 12embryonic stage 15embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic stage 13embryonic cycle 4embryonic stage 14embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pnut 

Pnut

bait
Septin1 

Sep1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Sep1 and pnut also cosediment from crude cell extracts at about 8 S, suggesting a molecular mass of 160-200 kDa.

(Fares et al., 1995)

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Fares et al., 1995)
FBrf0085016-2.coIP.WB
Reference
Fares et al., 1995
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 16blastoderm stagegastrula stageembryonic stage 8dorsal closure stageembryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3extended germ band stageembryonic stage 4embryonic cycle 9embryonic stage 2late extended germ band stageembryonic stage 5embryonic stage 11embryonic stage 3embryonic stage 12embryonic stage 15embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic stage 13embryonic cycle 4embryonic stage 14embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Septin1 

Sep1

bait
pnut 

Pnut

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Sep1 and pnut also cosediment from crude cell extracts at about 8 S, suggesting a molecular mass of 160-200 kDa.

(Fares et al., 1995)

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Fares et al., 1995)
FBrf0087109-1.coIP.WB
Reference
Field et al., 1996
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 9blastoderm stagegastrula stageembryonic stage 8embryonic stage 6embryonic cycle 3embryonic stage 4embryonic cycle 9embryonic stage 2embryonic stage 5embryonic stage 3embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic cycle 4embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pnut 

Pnut

bait
Septin1 

SEP1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Proteins immunopurified from embryonic high-speed supernatant.

(Field et al., 1996)

pnut, Sep1 and Sep2 copurify in about a 1:1:1 stoichiometry.

(Field et al., 1996)

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Field et al., 1996)
FBrf0087109-3.CS.MW
Reference
Field et al., 1996
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 9blastoderm stagegastrula stageembryonic stage 8embryonic stage 6embryonic cycle 3embryonic stage 4embryonic cycle 9embryonic stage 2embryonic stage 5embryonic stage 3embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic cycle 4embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pnut 

Pnut

neutral component
pnut 

Pnut

neutral component
pnut 

Pnut

neutral component
Septin1 

SEP1

neutral component
Septin1 

SEP1

neutral component
Septin1 

SEP1

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

pnut, Sep1 and Sep2 cosediment as a complex at about 8S in a 1:1:1 stoichiometry.

(Field et al., 1996)

Source was embryos of wild-type fly line; proteins produced from endogenous gene.

(Field et al., 1996)

Proteins immunopurified from embryonic high-speed supernatant using anti-pnut antibody, then subjected to gradient fractionation.

(Field et al., 1996)
FBrf0087109-6.CH.MW
Reference
Field et al., 1996
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 9blastoderm stagegastrula stageembryonic stage 8embryonic stage 6embryonic cycle 3embryonic stage 4embryonic cycle 9embryonic stage 2embryonic stage 5embryonic stage 3embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic cycle 4embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pnut 

Pnut

neutral component
pnut 

Pnut

neutral component
pnut 

Pnut

neutral component
Septin1 

SEP1

neutral component
Septin1 

SEP1

neutral component
Septin1 

SEP1

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Proteins immunopurified from embryonic high-speed supernatant using anti-pnut antibody, then subjected to gel filtration chromatography.

(Field et al., 1996)

pnut, Sep1 and Sep2 copurify as a complex at about a 1:1:1 stoichiometry.

(Field et al., 1996)

Source was embryos of wild-type fly line; proteins produced from endogenous gene.

(Field et al., 1996)
FBrf0218395-7813.DPiM
Reference
Guruharsha et al., 2011
Description
physical association
Assay
Collection
DPiM-2
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Septin1 
unspecified role
Septin1 
unspecified role
Septin1 
unspecified role
pnut 
unspecified role
pnut 
unspecified role
pnut 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 420.84070

FBrf0221463-1.coIP.WB
Reference
Su et al., 2013
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 6blastoderm stageembryonic stage 5embryonic stage 3embryonic cycle 6embryonic cycle 8embryonic cycle 5embryonic cycle 7embryonic cycle 4embryonic cycle 3embryonic stage 4embryonic stage 2embryonic cycle 9
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pnut 

Pnut

bait
Septin1 

Sep1

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from transgenic constructendogenous gene.

(Su et al., 2013)
FBrf0224592-1.PD.MS
Reference
Mavrakis et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Septin1 

DSep1

bait

His tag

pnut 

Pnut

prey

Strep tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Mavrakis et al., 2014)

Sep1, Sep2 and pnut were coexpressed and purified by sequential His then Strep affinity purifications.

(Mavrakis et al., 2014)
FBrf0224592-3.PD.MS
Reference
Mavrakis et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pnut 

Pnut

bait

Strep tag

Septin1 

DSep1

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Mavrakis et al., 2014)

Sep1, Sep2 and pnut were coexpressed and purified by sequential His then Strep affinity purifications.

(Mavrakis et al., 2014)
FBrf0227131-1.coIP.WB
Reference
Akhmetova et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pnut 

Pnut

bait

His tag

Septin1 

Sep1

prey
Septin1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Septin1 
GTPase domain
mutation decreasing interaction
aa 48aa 99aa 181

K48A

D99A

K181A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of baculovirus-infected cell line; bait produced from transfected construct; prey produced from transfected construct.

(Akhmetova et al., 2015)

Mutations to the GTPase domain of pnut (K155A, D206A, K288A) do not affect the interaction with Sep1.

(Akhmetova et al., 2015)

Only when combined in a triple mutant do mutations of the Sep1 GTPase domain (K48A, D99A, K181A) disrupt binding to pnut.

(Akhmetova et al., 2015)
FBrf0227131-3.PD.A
Reference
Akhmetova et al., 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pnut 

Pnut

bait

[35]S label

pnut 
Septin1 

Sep1

prey

[35]S label

Septin1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pnut 
C-terminal region
necessary binding region
aa 461-539
Septin1 
coiled coil domain
necessary binding region
aa 329-361
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro translation; prey produced and labeled by in vitro translation.

(Akhmetova et al., 2015)
FBrf0237546-1.PD.MW
Reference
Akhmetova et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pnut 

Pnut

bait

His tag

pnut 
Septin1 

Sep1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pnut 
phosphorylation target residues 509,517
mutation decreasing interaction
aa 509,517

T509E,S517E

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus and insect cell system; prey produced in baculovirus and insect cell system.

(Akhmetova et al., 2018)
FBrf0237546-3.coIP.WB
Reference
Akhmetova et al., 2018
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pnut 

Pnut

bait

FLAG tag

Septin1 

Sep1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Akhmetova et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (7)