Isolation and characterization of Df(2R)BSC267
Stacey Christensen, Kimberley Cook and Kevin Cook
Bloomington Stock Center
Indiana University
Df(2R)BSC267 was isolated as a FLP recombinase-induced recombination 
event involving P{XP}sut1d07339 and PBac{WH}pdm3f00828. The 
deletion was isolated as a chromosome lacking miniwhite markers in 
progeny of P{hsFLP}1, y1 w1118; 
P{XP}sut1d07339/PBac{WH}pdm3f00828 males crossed to w1118; 
P{hs-hid}2, wgSp-1/CyO females. These males were heat shocked as 
larvae as described in Parks et al., Nature Genetics 36: 288-292, 
2004 (FBrf0175003). This cross and crosses in preceding and 
succeeding generations maintained the original genetic background of 
the Exelixis insertion stocks (Thibault et al., Nature Genetics 36: 
283-287, 2004; FBrf0175002). The recombination event generated the 
genetic element P+PBac{XP5.WH5}BSC267 from the segment of 
P{XP}sut1d07339 to the left of its FRT site and the segment of 
PBac{WH}pdm3f00828 to the right of its FRT site. Its presence was 
verified using the PCR methods and primers described in Parks et al. 
The cytological breakpoints of Df(2R)BSC267 predicted from the 
Release 5 genomic coordinates of the transposable element insertions 
sites are 44A4;44C4. It failed to complement cul-4KG02900 and 
pnutXP.  Df(2R)FDD-0089200 is a synonym for Df(2R)BSC267.