Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

RNAi studies indicate that scra recruits pnut to the cleavage furrow.

Cells were treated with the proteasome inhibitor MG132 to increase the mitotic index of cells and enrich for protein complexes involved in cytokinesis.

Addition of importins inhibit pnut but not Sep2 binding to scra.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.