A mutant pnut cDNA is expressed under the control of a 1.7 kb native pnut promoter fused with N-terminal Tag:FLAG. The mutant polypeptide carries amino acid substitutions K155A, D206A and K288A.
lethal - all die before end of larval stage, with pnutXP
imaginal disc, with pnutXP
larval glial cell, with pnutXP
pnutK155A.D206A.K288A.pnut.Tag:FLAG fails to rescue pnutXP
pnutXP mutant larvae also carrying pnutK155A.D206A.K288A.pnut.T:Zzzz\FLAG show an indistinguishable phenotype from that of pnutXP single mutants.