FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
pnut, Dlip2
FlyBase ID
FBig0000107631
Interaction Network
Interactions Browser links
pnut network
Dlip2 network
Reported Interactions
FBrf0144953-15.Y2H
Reference
Shih et al., 2002
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pnut 

Pnut

bait

fused to LexA DNA-binding domain

pnut 
Dlip2 

Sip4

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pnut 
N-terminal region
sufficient binding region
aa 1-426

coordinates relative to pnut-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/B42-AD

(Shih et al., 2002)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Shih et al., 2002)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)