FlyBase Allele Report: Dmel\prel[UAS.Tag:HA]

General Information

Symbol

Dmel\prel^UAS.Tag:HA

Species

D. melanogaster

Name

FlyBase ID

FBal0241224

Feature type

allele

Associated gene

Dmel\prel

Associated Insertion(s)

Carried in Construct

P{UAS-prel.3HA}

Also Known As

UAS-prel::3HA

Key Links

Transgenic product class

wild_type

(Tsubouchi et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Tsubouchi et al., 2009)

Mutagen

in vitro construct

(Tsubouchi et al., 2009)

Progenitor genotype

Carried in construct

P{UAS-prel.3HA}

Cytology

Description

UAS regulatory sequences drive expression of prel which is tagged with a Tag:HA epitope.

(Tsubouchi et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

prel

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

abdominal dorsal multidendritic neuron ddaA | third instar larval stage, with Scer\GAL4^smid-C161

(Tsuyama et al., 2017)

abdominal dorsal multidendritic neuron ddaC | second instar larval stage, with Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

abdominal dorsal multidendritic neuron ddaC | third instar larval stage, with Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

abdominal dorsal multidendritic neuron ddaC | third instar larval stage, with Scer\GAL4^ppk.PU

(Tsuyama et al., 2017)

abdominal dorsal multidendritic neuron ddaE | third instar larval stage, with Scer\GAL4²²¹

(Tsuyama et al., 2017)

dendrite & abdominal anterior ventral multidendritic neuron vdaa, with Scer\GAL4^ppk.1.9

(Tsubouchi et al., 2009)

dendrite & class IV dendritic arborising neuron, with Scer\GAL4^ppk.1.9

(Tsubouchi et al., 2009)

dendrite & dorsal multidendritic neuron ddaC | somatic clone, with Scer\GAL4^ppk.1.9

(Tsubouchi et al., 2009)

dendrite | second instar larval stage, with Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

dendrite | third instar larval stage, with Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

dendrite | third instar larval stage, with Scer\GAL4²²¹

(Tsuyama et al., 2017)

dendrite | third instar larval stage, with Scer\GAL4^ppk.PU

(Tsuyama et al., 2017)

dendrite | third instar larval stage, with Scer\GAL4^smid-C161

(Tsuyama et al., 2017)

mitochondrion & dorsal multidendritic neuron ddaC | somatic clone, with Scer\GAL4^ppk.1.9

(Tsubouchi et al., 2009)

mitochondrion | third instar larval stage, with Scer\GAL4^ppk.PU

(Tsuyama et al., 2017)

Detailed Description

Statement

Reference

Expression of prel^{Scer\UAS.T:Ivir\HA1} results in mitochondria fragmentation and dendritic arbor shortening in the class IV ddaC neurons (with the Scer\GAL4^ppk.PU driver) and similar though much weaker reduction in total dendrite branch length is also observed in class III ddaA (with Scer\GAL4^smid-C161) and class I ddaE (with Scer\GAL4²²¹) neurons in third instar larvae.

At early first larval instar no significant effect on dendritic patterning of class IV ddaC neurons is observed upon Scer\GAL4^109(2)80-driven expression of prel^{Scer\UAS.T:Ivir\HA1} but by early second instar over 70% of dendrite endings are regressed - significantly more than in controls.

(Tsuyama et al., 2017)

The density of mitochondria (assayed by the expression of markers with a mitochondrial targeting signal) is significantly reduced in both axons and dendrites of ddaC neuron clones expressing prel^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^ppk.1.9 compared to controls. The mitochondria appear to be fragmented in the mutant neurons.

Expression of prel^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^ppk.1.9 in ddaC neuron clones results in smaller, simpler dendritic arbors.

Class IV v'ada neurons in the adult abdomen that are expressing prel^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^ppk.1.9 show a significant reduction in the total length of dendritic branches compared to controls and the neurons show late-onset retraction of their arbors when observed at 10 and 30 days as adults.

(Tsubouchi et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has abnormal neuroanatomy | third instar larval stage phenotype, enhanceable by bsk^DN.UAS/Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has abnormal neuroanatomy | third instar larval stage phenotype, enhanceable by PPP1R15^UAS.cMa, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Suppressed by

Statement

Reference

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by PPP1R15^UAS.cMa, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by PEK^GL00030, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by PEK^KK100348, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by PEK^GD5584, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by PEK^HMJ02063, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by BacA\p35^UAS.cHa, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

NOT suppressed by

Statement

Reference

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by AMPKα^K57A.UAS, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Scer\GAL4^109(2)80/p53^H159N.UAS.Ex

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Scer\GAL4^109(2)80/Sod1^UAS.cAa

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by S6k^STDETE.UAS, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Gcn2^GL00267, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Gcn2^KK103566, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Gcn2^GD9162, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Scer\GAL4^109(2)80/Thor^GL01034

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Scer\GAL4^109(2)80/Thor^HMS01555

(Tsuyama et al., 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has abdominal dorsal multidendritic neuron ddaC | third instar larval stage phenotype, enhanceable by bsk^DN.UAS/Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has dendrite | third instar larval stage phenotype, enhanceable by bsk^DN.UAS/Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has abdominal dorsal multidendritic neuron ddaC | third instar larval stage phenotype, enhanceable by PPP1R15^UAS.cMa, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has dendrite | third instar larval stage phenotype, enhanceable by PPP1R15^UAS.cMa, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Suppressed by

Statement

Reference

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has abdominal dorsal multidendritic neuron ddaC | third instar larval stage phenotype, suppressible | partially by BacA\p35^UAS.cHa, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has dendrite | third instar larval stage phenotype, suppressible | partially by BacA\p35^UAS.cHa, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has abdominal dorsal multidendritic neuron ddaC | third instar larval stage phenotype, suppressible | partially by PPP1R15^UAS.cMa, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has dendrite | third instar larval stage phenotype, suppressible | partially by PPP1R15^UAS.cMa, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has abdominal dorsal multidendritic neuron ddaC | third instar larval stage phenotype, suppressible | partially by PEK^GL00030, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has dendrite | third instar larval stage phenotype, suppressible | partially by PEK^GL00030, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has abdominal dorsal multidendritic neuron ddaC | third instar larval stage phenotype, suppressible | partially by PEK^KK100348, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has dendrite | third instar larval stage phenotype, suppressible | partially by PEK^KK100348, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has abdominal dorsal multidendritic neuron ddaC | third instar larval stage phenotype, suppressible | partially by PEK^GD5584, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has dendrite | third instar larval stage phenotype, suppressible | partially by PEK^GD5584, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has abdominal dorsal multidendritic neuron ddaC | third instar larval stage phenotype, suppressible | partially by PEK^HMJ02063, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has dendrite | third instar larval stage phenotype, suppressible | partially by PEK^HMJ02063, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

NOT suppressed by

Statement

Reference

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has dendrite | third instar larval stage phenotype, non-suppressible by AMPKα^K57A.UAS, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has abdominal dorsal multidendritic neuron ddaC | third instar larval stage phenotype, non-suppressible by Scer\GAL4^109(2)80/p53^H159N.UAS.Ex

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has dendrite | third instar larval stage phenotype, non-suppressible by Scer\GAL4^109(2)80/p53^H159N.UAS.Ex

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has abdominal dorsal multidendritic neuron ddaC | third instar larval stage phenotype, non-suppressible by Scer\GAL4^109(2)80/Sod1^UAS.cAa

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has dendrite | third instar larval stage phenotype, non-suppressible by Scer\GAL4^109(2)80/Sod1^UAS.cAa

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has mitochondrion | third instar larval stage phenotype, non-suppressible by PPP1R15^UAS.cMa, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has abdominal dorsal multidendritic neuron ddaC | third instar larval stage phenotype, non-suppressible by S6k^STDETE.UAS, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has dendrite | third instar larval stage phenotype, non-suppressible by S6k^STDETE.UAS, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has abdominal dorsal multidendritic neuron ddaC | third instar larval stage phenotype, non-suppressible by Gcn2^GL00267, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has dendrite | third instar larval stage phenotype, non-suppressible by Gcn2^GL00267, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has abdominal dorsal multidendritic neuron ddaC | third instar larval stage phenotype, non-suppressible by Gcn2^KK103566, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has dendrite | third instar larval stage phenotype, non-suppressible by Gcn2^KK103566, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has abdominal dorsal multidendritic neuron ddaC | third instar larval stage phenotype, non-suppressible by Gcn2^GD9162, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has dendrite | third instar larval stage phenotype, non-suppressible by Gcn2^GD9162, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has abdominal dorsal multidendritic neuron ddaC | third instar larval stage phenotype, non-suppressible by Scer\GAL4^109(2)80/Thor^GL01034

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has dendrite | third instar larval stage phenotype, non-suppressible by Scer\GAL4^109(2)80/Thor^GL01034

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has abdominal dorsal multidendritic neuron ddaC | third instar larval stage phenotype, non-suppressible by Scer\GAL4^109(2)80/Thor^HMS01555

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has dendrite | third instar larval stage phenotype, non-suppressible by Scer\GAL4^109(2)80/Thor^HMS01555

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80, prel^UAS.Tag:HA has abdominal dorsal multidendritic neuron ddaC | third instar larval stage phenotype, non-suppressible by AMPKα^K57A.UAS, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The shortening of the dendritic arbor in class IV ddaC neurons in third instar larvae expressing prel^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^109(2)80 is slightly enhanced by co-expression of bsk^DN.Scer\UAS, is not significantly affected by co-expression of any of the following: AMPKα^{K57A.Scer\UAS}, p53^{H159N.Scer\UAS.Ex}, Sod1^Scer\UAS.cAa, S6k^{STDETE.Scer\UAS}, Gcn2^GL00267, Gcn2^KK103566, Gcn2^GD9162, Thor^GL01034 or Thor^HMS01555 but is strongly mitigated by co-expression of any of the following: PEK[GL00030], PEK^KK100348, PEK^GD5584 or PEK^HMJ02063 or PPP1R15^Scer\UAS.cMa, which however in some cases has the opposite effect and appears to enhance rather than suppress the dendritic loss phenotype.

The mitochondria fragmentation induced by prel^{Scer\UAS.T:Ivir\HA1} expression however is not improved by PPP1R15^Scer\UAS.cMa co-expression.

(Tsuyama et al., 2017)

Xenogenetic Interactions

Statement

Reference

The shortening of the dendritic arbor in class IV ddaC neurons in third instar larvae expressing prel^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^109(2)80 is only slightly mitigated by co-expression of BacA\p35^Scer\UAS.cHa.

(Tsuyama et al., 2017)

Complementation and Rescue Data

Rescues

Scer\GAL4^da.G32/prel^UAS.Tag:HA rescues prel¹

(Tsubouchi et al., 2009)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

prel^{Scer\UAS.T:Ivir\HA1}

prel^UAS.Tag:HA

Name Synonyms

Secondary FlyBase IDs

References (2)

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