Expression of SNF1AK57A.Scer\UAS under the control of Scer\GAL4109(2)80 results in aberrant morphology in class IV multi-dendritic neurons.
Expression of one or two copies of SNF1AK57A.Scer\UAS under the control of Scer\GAL4Ubi significantly reduces adult lifespan. Co-expression of SNF1AScer\UAS.cMa with SNF1AK57A.Scer\UAS attenuates starvation sensitivity phenotypes.
Heat-shock induced expression of SNF1AK57A.Scer\UAS (under the control of Scer\GAL4hs.PB) in adult stages produces a significant reduction in starvation survival. Starvation sensitivity is indistinguishable in these animals from controls. These animals also exhibit a significant reduction in lifespan under nutrient-rich conditions.
Ubiquitous expression of SNF1AK57A.Scer\UAS (under the control of Scer\GAL4Ubi) results in significantly lower amounts of locomotion during normal, unstressed conditions as compared to controls. However, these flies show an instant increase in activity levels when faced with starvation, as oppose to a steady increase as found in wild-type controls, indicating lower locomotion levels in a fed-state, and elevated locomotion levels in a starvation state.
Total daily food intake is significantly increased in animals expressing SNF1AK57A.Scer\UAS under the control of Scer\GAL4Ubi, independent of nutritional value. Specifically, in both males and females, total dietary intake is nearly twice that of animals with wild-type SNF1A function.
Under fed conditions, larvae expressing SNF1AK57A.Scer\UAS under the control of Scer\GAL4Ubi show significantly more and larger lipid droplets in oenocytes compared to controls, resembling the starved phenotype of wild-type oenocytes.
While there is no overt difference in weight, there is a significant impact of SNF1AK57A.Scer\UAS expression, under the control of Scer\GAL4Ubi, on total triglyceride stores under fed conditions; with the average amount of triglyceride levels for the SNF1AK57A.Scer\UAS flies being significantly lower than control flies.
Measurements of O[] consumption in animals expressing SNF1AK57A.Scer\UAS, under the control of Scer\GAL4Ubi, are consistently higher than in control animals.
Feeding SNF1AK57A.Scer\UAS expressing flies (under the control of Scer\GAL4Ubi) with Rapamycin improves their starvation sensitivity. The median survival of these animals is significantly improved during starvation conditions for females and males.