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General Information
Symbol
Dmel\AMPKαK57A.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0246548
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of a kinase dead version of AMPKα with a K57A amino acid substitution.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Ectopic expression of AMPKαK57A.Scer\UAS under the control of Scer\GAL4Ubi.PU results in partial lethality, with majority being viable at 25[o]C but only about a fifth at 30[o]C.

Expression of SNF1AK57A.Scer\UAS under the control of Scer\GAL4109(2)80 results in aberrant morphology in class IV multi-dendritic neurons.

Expression of one or two copies of SNF1AK57A.Scer\UAS under the control of Scer\GAL4Ubi significantly reduces adult lifespan. Co-expression of SNF1AScer\UAS.cMa with SNF1AK57A.Scer\UAS attenuates starvation sensitivity phenotypes.

Heat-shock induced expression of SNF1AK57A.Scer\UAS (under the control of Scer\GAL4hs.PB) in adult stages produces a significant reduction in starvation survival. Starvation sensitivity is indistinguishable in these animals from controls. These animals also exhibit a significant reduction in lifespan under nutrient-rich conditions.

Ubiquitous expression of SNF1AK57A.Scer\UAS (under the control of Scer\GAL4Ubi) results in significantly lower amounts of locomotion during normal, unstressed conditions as compared to controls. However, these flies show an instant increase in activity levels when faced with starvation, as oppose to a steady increase as found in wild-type controls, indicating lower locomotion levels in a fed-state, and elevated locomotion levels in a starvation state.

Total daily food intake is significantly increased in animals expressing SNF1AK57A.Scer\UAS under the control of Scer\GAL4Ubi, independent of nutritional value. Specifically, in both males and females, total dietary intake is nearly twice that of animals with wild-type SNF1A function.

Under fed conditions, larvae expressing SNF1AK57A.Scer\UAS under the control of Scer\GAL4Ubi show significantly more and larger lipid droplets in oenocytes compared to controls, resembling the starved phenotype of wild-type oenocytes.

While there is no overt difference in weight, there is a significant impact of SNF1AK57A.Scer\UAS expression, under the control of Scer\GAL4Ubi, on total triglyceride stores under fed conditions; with the average amount of triglyceride levels for the SNF1AK57A.Scer\UAS flies being significantly lower than control flies.

Measurements of O[[2]] consumption in animals expressing SNF1AK57A.Scer\UAS, under the control of Scer\GAL4Ubi, are consistently higher than in control animals.

Feeding SNF1AK57A.Scer\UAS expressing flies (under the control of Scer\GAL4Ubi) with Rapamycin improves their starvation sensitivity. The median survival of these animals is significantly improved during starvation conditions for females and males.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement
Reference
NOT Suppressor of
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The shortening of the dendritic arbor in class IV ddaC neurons in third instar larvae expressing prelScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4109(2)80 is not significantly affected by co-expression of AMPKαK57A.Scer\UAS.

Expression of SNF1AK57A.Scer\UAS under the control of Scer\GAL4AdSS-F71 suppresses the increased longevity of AdSSF71/+ males and females.

Xenogenetic Interactions
Statement
Reference

Expression of SNF1AK57A.Scer\UAS enhances the climbing defects seen in flies expressing Hsap\LRRK2G2019S.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4Ddc.PL. The beneficial effects of EGCG treatment on climbing ability are completely abolished. The beneficial effects of AICAR on climbing ability and protocerebral posterior lateral 1 (PPL1) dopaminergic neuron loss are also abolished.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
AMPKαK57A.Scer\UAS
AMPKαK57A.UAS
SNF1AK57A.Scer\UAS
Name Synonyms
Secondary FlyBase IDs
    References (9)