FlyBase Allele Report: Dmel\PPP1R15[UAS.cMa]

General Information

Symbol

Dmel\PPP1R15^UAS.cMa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Malzer

FlyBase ID

FBal0284372

Feature type

allele

Associated gene

Dmel\PPP1R15

Associated Insertion(s)

Carried in Construct

M{UAS-PPP1R15.M}

Also Known As

UAS-GADD34, UAS-dPPP1R15

Key Links

Transgenic product class

cDNA

(Malzer et al., 2013)

wild_type

(Malzer et al., 2013)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Malzer et al., 2013)

wild_type

(Malzer et al., 2013)

Mutagen

in vitro construct

(Malzer et al., 2013)

Progenitor genotype

Carried in construct

M{UAS-PPP1R15.M}

Cytology

Description

UASt regulatory sequences drive expression of the PPP1R15 GH11727 cDNA clone.

(Malzer et al., 2013)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

PPP1R15

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates syndrome

modeled by RpS23^R67K

(Recasens-Alvarez et al., 2021)

ameliorates Alzheimer's disease 1

modeled by Hsap\APP^{Aβ42.UAS.Tag:SS(rPENK)}

(Cheng and Chiang, 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased size | adult stage, with Scer\GAL4^nub.PK

(Recasens-Alvarez et al., 2021)

visible | adult stage, with Scer\GAL4^nub.PK

(Recasens-Alvarez et al., 2021)

Phenotype Manifest In

wing, with Scer\GAL4^nub.PK

(Recasens-Alvarez et al., 2021)

Detailed Description

Statement

Reference

The expression of PPP1R15^UAS.cMa under the control of Scer\GAL4^nub.PK induces a large wing.

(Recasens-Alvarez et al., 2021)

Expression of PPP1R15^Scer\UAS.cMa under the control of Scer\GAL4^109(2)80 does not significantly affect morphology of either mitochondria or the dendritic arbor in class IV ddaC neurons in third instar larvae.

(Tsuyama et al., 2017)

Expression of Gadd34^Scer\UAS.cMa under the control of Scer\GAL4^GMR.PF does not result in a mutant eye phenotype.

(Malzer et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Scer\GAL4^nub.PK/PPP1R15^UAS.cMa is an enhancer of increased cell death | larval stage | somatic clone phenotype of RpS23^R67K

(Recasens-Alvarez et al., 2021)

PPP1R15^UAS.cMa, Scer\GAL4^109(2)80 is an enhancer of abnormal neuroanatomy | third instar larval stage phenotype of Scer\GAL4^109(2)80, prel^UAS.Tag:HA

(Tsuyama et al., 2017)

PPP1R15^UAS.cMa, Scer\GAL4^109(2)80 is an enhancer of abnormal neuroanatomy | third instar larval stage phenotype of Scer\GAL4^109(2)80, TFAM^UAS.cCa

(Tsuyama et al., 2017)

Suppressor of

Statement

Reference

PPP1R15^UAS.cMa, Scer\GAL4^hh-Gal4 is a suppressor of increased cell death | larval stage phenotype of Rer1^GD13262, Scer\GAL4^hh-Gal4

(Paul et al., 2024)

PPP1R15^UAS.cMa/Scer\GAL4^αTub84B.PL is a suppressor of decreased size | somatic clone | larval stage phenotype of Rer1^KO

(Paul et al., 2024)

PPP1R15^UAS.cMa/Scer\GAL4^αTub84B.PL is a suppressor of increased cell death | somatic clone | larval stage phenotype of Rer1^KO

(Paul et al., 2024)

PPP1R15^UAS.cMa, Scer\GAL4^109(2)80 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Opa1^UAS.Tag:HA, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

Scer\GAL4^109(2)80/PPP1R15^UAS.cMa is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Opa1^{K273A.UAS.Tag:HA}, Scer\GAL4^ppk.PU

(Tsuyama et al., 2017)

PPP1R15^UAS.cMa, Scer\GAL4^109(2)80 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Scer\GAL4^109(2)80, ttm50^UAS.Tag:HA

(Tsuyama et al., 2017)

PPP1R15^UAS.cMa, Scer\GAL4^109(2)80 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of COX5A^tend, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

PPP1R15^UAS.cMa, Scer\GAL4^109(2)80 is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Scer\GAL4^109(2)80, TFAM^UAS.cCa

(Tsuyama et al., 2017)

PPP1R15^UAS.cMa/Scer\GAL4^ppk.PU is a suppressor | partially of abnormal neuroanatomy | third instar larval stage phenotype of prel¹

(Tsuyama et al., 2017)

PPP1R15^UAS.cMa, Scer\GAL4^109(2)80 is a suppressor | partially of abnormal neuroanatomy | third instar larval stage phenotype of Scer\GAL4^109(2)80, prel^UAS.Tag:HA

(Tsuyama et al., 2017)

PPP1R15^UAS.cMa, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of PEK^UAS.cMa, Scer\GAL4^GMR.PF

(Malzer et al., 2013)

NOT Suppressor of

Statement

Reference

Scer\GAL4^nub.PK/PPP1R15^UAS.cMa is a non-suppressor of decreased size | adult stage phenotype of RpS23^R67K

(Recasens-Alvarez et al., 2021)

Scer\GAL4^nub.PK/PPP1R15^UAS.cMa is a non-suppressor of visible | adult stage phenotype of RpS23^R67K

(Recasens-Alvarez et al., 2021)

Phenotype Manifest In

Enhancer of

Statement

Reference

Scer\GAL4^nub.PK/PPP1R15^UAS.cMa is an enhancer of imaginal disc phenotype of RpS23^R67K

(Recasens-Alvarez et al., 2021)

PPP1R15^UAS.cMa, Scer\GAL4^109(2)80 is an enhancer of abdominal dorsal multidendritic neuron ddaC | third instar larval stage phenotype of Scer\GAL4^109(2)80, prel^UAS.Tag:HA

(Tsuyama et al., 2017)

PPP1R15^UAS.cMa, Scer\GAL4^109(2)80 is an enhancer of dendrite | third instar larval stage phenotype of Scer\GAL4^109(2)80, prel^UAS.Tag:HA

(Tsuyama et al., 2017)

PPP1R15^UAS.cMa, Scer\GAL4^109(2)80 is an enhancer of abdominal dorsal multidendritic neuron ddaC | third instar larval stage phenotype of Scer\GAL4^109(2)80, TFAM^UAS.cCa

(Tsuyama et al., 2017)

PPP1R15^UAS.cMa, Scer\GAL4^109(2)80 is an enhancer of dendrite | third instar larval stage phenotype of Scer\GAL4^109(2)80, TFAM^UAS.cCa

(Tsuyama et al., 2017)

Suppressor of

Statement

Reference

PPP1R15^UAS.cMa, Scer\GAL4^hh-Gal4 is a suppressor of wing disc phenotype of Rer1^GD13262, Scer\GAL4^hh-Gal4

(Paul et al., 2024)

PPP1R15^UAS.cMa/Scer\GAL4^αTub84B.PL is a suppressor of wing disc | somatic clone phenotype of Rer1^KO

(Paul et al., 2024)

PPP1R15^UAS.cMa, Scer\GAL4^109(2)80 is a suppressor | partially of abdominal dorsal multidendritic neuron ddaC | third instar larval stage phenotype of Scer\GAL4^109(2)80, prel^UAS.Tag:HA

(Tsuyama et al., 2017)

PPP1R15^UAS.cMa, Scer\GAL4^109(2)80 is a suppressor | partially of dendrite | third instar larval stage phenotype of Scer\GAL4^109(2)80, prel^UAS.Tag:HA

(Tsuyama et al., 2017)

PPP1R15^UAS.cMa, Scer\GAL4^109(2)80 is a suppressor of abdominal dorsal multidendritic neuron ddaC | third instar larval stage phenotype of Opa1^UAS.Tag:HA, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

PPP1R15^UAS.cMa, Scer\GAL4^109(2)80 is a suppressor of dendrite | third instar larval stage phenotype of Opa1^UAS.Tag:HA, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

PPP1R15^UAS.cMa, Scer\GAL4^109(2)80 is a suppressor of abdominal dorsal multidendritic neuron ddaC | third instar larval stage phenotype of Opa1^{K273A.UAS.Tag:HA}, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

PPP1R15^UAS.cMa, Scer\GAL4^109(2)80 is a suppressor of dendrite | third instar larval stage phenotype of Opa1^{K273A.UAS.Tag:HA}, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

PPP1R15^UAS.cMa, Scer\GAL4^109(2)80 is a suppressor of abdominal dorsal multidendritic neuron ddaC | third instar larval stage phenotype of Scer\GAL4^109(2)80, ttm50^UAS.Tag:HA

(Tsuyama et al., 2017)

PPP1R15^UAS.cMa, Scer\GAL4^109(2)80 is a suppressor of dendrite | third instar larval stage phenotype of Scer\GAL4^109(2)80, ttm50^UAS.Tag:HA

(Tsuyama et al., 2017)

PPP1R15^UAS.cMa, Scer\GAL4^109(2)80 is a suppressor of abdominal dorsal multidendritic neuron ddaC | third instar larval stage phenotype of COX5A^tend, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

PPP1R15^UAS.cMa, Scer\GAL4^109(2)80 is a suppressor of dendrite | third instar larval stage phenotype of COX5A^tend, Scer\GAL4^109(2)80

(Tsuyama et al., 2017)

PPP1R15^UAS.cMa, Scer\GAL4^109(2)80 is a suppressor of abdominal dorsal multidendritic neuron ddaC | third instar larval stage phenotype of Scer\GAL4^109(2)80, TFAM^UAS.cCa

(Tsuyama et al., 2017)

PPP1R15^UAS.cMa, Scer\GAL4^109(2)80 is a suppressor of dendrite | third instar larval stage phenotype of Scer\GAL4^109(2)80, TFAM^UAS.cCa

(Tsuyama et al., 2017)

PPP1R15^UAS.cMa/Scer\GAL4^ppk.PU is a suppressor | partially of abdominal dorsal multidendritic neuron ddaC | third instar larval stage phenotype of prel¹

(Tsuyama et al., 2017)

PPP1R15^UAS.cMa/Scer\GAL4^ppk.PU is a suppressor | partially of dendrite | third instar larval stage phenotype of prel¹

(Tsuyama et al., 2017)

PPP1R15^UAS.cMa, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of PEK^UAS.cMa, Scer\GAL4^GMR.PF

(Malzer et al., 2013)

NOT Suppressor of

Statement

Reference

Scer\GAL4^nub.PK/PPP1R15^UAS.cMa is a non-suppressor of wing phenotype of RpS23^R67K

(Recasens-Alvarez et al., 2021)

PPP1R15^UAS.cMa, Scer\GAL4^109(2)80 is a non-suppressor of mitochondrion | third instar larval stage phenotype of Scer\GAL4^109(2)80, prel^UAS.Tag:HA

(Tsuyama et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

The shortening of class IV ddaC dendritic arbor characteristic observed in prel¹ mutant third instar larvae can be significantly mitigated by expression of PPP1R15^Scer\UAS.cMa under the control of Scer\GAL4^ppk.PU. Similarly, co-expression of PPP1R15^Scer\UAS.cMa strongly suppresses the reduced dendritic arborization induced by Scer\GAL4^109(2)80-driven expression of either prel^{Scer\UAS.T:Ivir\HA1} or TFAM^Scer\UAS.cCa (in some cases however this leads to the opposite phenotype and the dendritic loss is enhanced rather than suppressed) as well as any of the following: Opa1^{Scer\UAS.T:Ivir\HA1}, Opa1^{K273A.Scer\UAS.T:Ivir\HA1}, ttm50^{Scer\UAS.T:Ivir\HA1} or COX5A^tend.

(Tsuyama et al., 2017)

Co-expression of Gadd34^Scer\UAS.cMa suppresses the eye defects caused by expression of PEK^Scer\UAS.cMa under the control of Scer\GAL4^GMR.PF.

(Malzer et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

76250

y¹ w^*; M{UAS-PPP1R15.M}ZH-51D/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Gadd34^Scer\UAS.cMa

PPP1R15^Scer\UAS.cMa

PPP1R15^UAS.cMa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Malzer

Secondary FlyBase IDs

References (5)

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