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General Information
Symbol
Dmel\numbTS4D.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0268717
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Numb-TS4D
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of numb sequences where five putative phospho-sites (T143, S183, S188, S248 and S447) have been mutated to be phosphomimetic (i.e. appear always phosphorylated).

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Larval brain clones expressing numbTS4D.Scer\UAS under the control of Scer\GAL4Act.PU exhibit ectopic type II neuroblasts.

Overexpression of numbTS4D.Scer\UAS in larval brain neuroblasts under the control of Scer\GAL4insc-Mz1407 leads to a very dramatic increase in type II neuroblast number (compared to expression of numbS2D.Scer\UAS). While neuroblasts are more proliferative in these mutants, the number of elav-positive postmitotic neurons. numb localisation into basal crescents in these neuroblasts appears normal, although some show compromised localisation at metaphase and telophase.

Expression of numbTS4D.Scer\UAS in the type-I neuroblast lineage under the control of Scer\GAL4ase.neuro has no effect on neuroblast number.

Scer\GAL4erm.R9D11-driven expression of numbTS4D.Scer\UAS in mature INPs, GMCs, and differentiated neurons fails to induce ectopic neuroblast formation.

Type-II neuroblasts clones expressing numbTS4D.Scer\UAS contain ectopic neuroblasts, whereas numbTS4D.Scer\UAS-expressing type-I neuroblast clones always contain a single neuroblast, as found in controls.

Expression of numbTS4D.Scer\UAS under the control of Scer\GAL4insc-Mz1407 induces supernumerary neuroblast formation.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
NOT suppressed by
Suppressor of
Other
Phenotype Manifest In
Enhanced by
Suppressed by
NOT suppressed by
Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of bansponge.Scer\UAS.T:Disc\RFP, but not banD.Scer\UAS.T:Avic\GFP-EGFP, suppresses the ectopic type II neuroblast phenotype seen in larval brain clones expressing numbTS4D.Scer\UAS under the control of Scer\GAL4Act.PU.

Expression of Ncpro.Scer\UAS attenuates numbTS4D.Scer\UAS induced ectopic neuroblast formation.

Expression of NcΔN.Scer\UAS attenuates numbTS4D.Scer\UAS induced ectopic neuroblast formation. No change in apoptosis is reported in this background, although the number of postmitotic neurons is increased.

Co-expression of ponS611D.Scer\UAS under the control of Scer\GAL4insc-Mz1407 rescues numbTS4D.Scer\UAS localisation at metaphase and telophase, but is unable to rescue ectopic type II neuroblast formation.

numbTS4D.Scer\UAS induced ectopic neuroblast formation is abolished in spdoG104 mutant clones.

Inhibition of N activity through Nl1N-ts1 suppresses numbTS4D.Scer\UAS induced ectopic neuroblast formation.

Inhibition of N activity through NGD144 suppresses numbTS4D.Scer\UAS induced ectopic neuroblast formation.

Co-expression of p53Scer\UAS.Ex suppresses ectopic neuroblast formation induced by numbTS4D.Scer\UAS expression under the control of Scer\GAL4insc-Mz1407.

Co-expression of p53H159N.Scer\UAS.Ex (that lacks transactivation activity) does not suppress supernumerary neuroblast formation induced by numbTS4D.Scer\UAS expression under the control of Scer\GAL4insc-Mz1407.

Larval brains expressing numbTS4D.Scer\UAS and p53Scer\UAS.Ex under the control of Scer\GAL4insc-Mz1407 exhibit an overall increase in apoptosis compared to controls (as visualised by TUNEL). However, neuroblast apoptosis remains rare, indicating that the apoptosis increase is in post-mitotic neurons or other brain cells.

A dapunspecified mutant background does not attenuate the ability of p53Scer\UAS.Ex expression to suppress the formation of ectopic neuroblasts induced by numbTS4D.Scer\UAS expression (under the control of Scer\GAL4insc-Mz1407).

Xenogenetic Interactions
Statement
Reference

Co-expression of BacA\p35Scer\UAS.cHa does not affect the suppression of ectopic neuroblast formation by p53Scer\UAS.Ex in numbTS4D.Scer\UAS-expressing larval brains (all transgenes under the control of Scer\GAL4insc-Mz1407).

Complementation and Rescue Data
Fails to rescue
Comments

Expression of numbTS4D.Scer\UAS fails to suppress the ectopic neuroblast formation seen in numb15 mutant clones.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
numbTS4D.Scer\UAS
numbTS4D.UAS
Name Synonyms
Secondary FlyBase IDs
    References (4)