FlyBase Allele Report: Dmel\p53[UAS.Ex]

General Information

Symbol

Dmel\p53^UAS.Ex

Species

D. melanogaster

Name

FlyBase ID

FBal0117412

Feature type

allele

Associated gene

Dmel\p53

Associated Insertion(s)

Carried in Construct

P{UAS-p53.Ex}

Also Known As

UAS-p53, UAS-Dmp53, UAS-dp53

Key Links

Transgenic product class

wild_type

(Ollmann et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Ollmann et al., 2000)

Mutagen

in vitro construct

(Ollmann et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-p53.Ex}

Cytology

Description

Expression of p53 open reading frame is driven by the UAS regulatory sequences. The alpha tubulin 3' UTR provides stability to the message.

(Ollmann et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

p53

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate, with Scer\GAL4^Ilp2.PR

(Hasygar and Hietakangas, 2014)

abnormal size, with Scer\GAL4^GMR.PF

(Zhang et al., 2015)

abnormal size | adult stage, with Scer\GAL4^GMR.PU

(Ren et al., 2017)

decreased body size | pupal stage, with Scer\GAL4^Ilp2.PR

(Hasygar and Hietakangas, 2014)

increased cell death, with Scer\GAL4^GMR.PF

(Zhang et al., 2015, Morishita et al., 2013, Peterson et al., 2002)

increased cell death, with Scer\GAL4^GMR.PU

(Kim et al., 2011)

increased cell death, with Scer\GAL4^hs.PU

(Mehrotra et al., 2008)

visible, with Scer\GAL4^GMR.PF

(Zhang et al., 2015, Peterson et al., 2002)

visible, with Scer\GAL4^GMR.PU

(Kim et al., 2012)

visible | adult stage, with Scer\GAL4^GMR.PU

(Ren et al., 2017)

Phenotype Manifest In

eye, with Scer\GAL4^ey.PH

(Singh et al., 2006)

eye, with Scer\GAL4^GMR.PF

(Zhang et al., 2015, Morishita et al., 2013, Pardi et al., 2011, Peterson et al., 2002)

eye, with Scer\GAL4^GMR.PU

(Ren et al., 2017, Kim et al., 2012, Kim et al., 2011)

eye disc, with Scer\GAL4^GMR.PU

(Kim et al., 2011)

follicle cell | oogenesis stage S8, with Scer\GAL4^hs.PU

(Mehrotra et al., 2008)

imaginal disc, with Scer\GAL4^hs.PU

(Mehrotra et al., 2008)

neuroblast, with Scer\GAL4^insc-Mz1407

(Ouyang et al., 2011)

ommatidium, with Scer\GAL4^GMR.PF

(Zhang et al., 2015)

Detailed Description

Statement

Reference

The expression of p53^Scer\UAS.Ex under the control of Scer\GAL4^GMR.PU leads to a decrease in eye size, as compared to controls.

(Ren et al., 2017)

Expression of p53^Scer\UAS.Ex in the eye under the control of Scer\GAL4^GMR.PF induces vast cell death and produces small and rough eyes with fused ommatidia.

(Zhang et al., 2015)

Scer\GAL4^Ilp2.PR-mediated, but not Scer\GAL4^Sgs3.PD-mediated expression results in delayed pupation and a significant reduction in pupal volume compared to controls.

(Hasygar and Hietakangas, 2014)

Expression of p53^Scer\UAS.Ex in the developing eye, under the control of Scer\GAL4^GMR.PF, causes a partial ablation of the eye due to excessive cell death during eye development.

(Morishita et al., 2013)

Expression of p53^Scer\UAS.Ex under the control of Scer\GAL4^GMR.PU results in a significant reduction in eye size and a disruption of ommatidial structure.

(Kim et al., 2012)

Scer\GAL4^GMR.PU-mediated expression of p53^Scer\UAS.Ex results in a disrupted adult eye, and increased apoptosis in the larval eye disc.

(Kim et al., 2011)

Expression of p53^Scer\UAS.Ex under the control of Scer\GAL4^insc-Mz1407 reduces neuroblast number by approximately 50%, compared to wild-type.

(Ouyang et al., 2011)

Overexpression of p53^Scer\UAS.Ex in the developing eye under the control of Scer\GAL4^GMR.PF generates a rough eye phenotype.

(Pardi et al., 2011)

Expression of p53^Scer\UAS.Ex under the control of Scer\GAL4^hs.PU induces apoptosis in up to 18% of mitotically cycling imaginal disc cells, but not in any endocycling cells of salivary glands, fat body, or gut. Similar results are obtained in the ovary, where overexpression of p53 induces apoptosis in mitotically cycling but not endocycling follicle cells.

(Mehrotra et al., 2008)

Expression of p53^Scer\UAS.Ex under the control of Scer\GAL4^ey.PH results in reduced eyes.

(Singh et al., 2006)

Expression of p53^Scer\UAS.Ex under the control of Scer\GAL4^GMR.PF does not affect eye pigmentation levels.

(Pankotai et al., 2005)

Flies expressing p53^Scer\UAS.Ex under the control of Scer\GAL4^GMR.PF have small eyes due to ectopic apoptosis.

(Peterson et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PU, p53^UAS.Ex has increased cell death phenotype, enhanceable by Hr3^RA.UAS, Scer\GAL4^GMR.PU

(Kim et al., 2011)

Scer\GAL4^GMR.PU, p53^UAS.Ex has increased cell death phenotype, enhanceable by Hr3^RB.UAS, Scer\GAL4^GMR.PU

(Kim et al., 2011)

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, p53^UAS.Ex has increased cell death phenotype, non-enhanceable by Df(3L)H99/Df(3L)XR38

(Peterson et al., 2002)

Scer\GAL4^GMR.PF, p53^UAS.Ex has visible phenotype, non-enhanceable by Df(3L)H99/Df(3L)XR38

(Peterson et al., 2002)

Suppressed by

Statement

Reference

Scer\GAL4^Ilp2.PR, p53^UAS.Ex has decreased body size | pupal stage phenotype, suppressible | partially by Erk7^KK102574, Scer\GAL4^Ilp2.PR

(Hasygar and Hietakangas, 2014)

Scer\GAL4^Ilp2.PR, p53^UAS.Ex has abnormal developmental rate phenotype, suppressible | partially by Erk7^KK102574, Scer\GAL4^Ilp2.PR

(Hasygar and Hietakangas, 2014)

Scer\GAL4^Ilp2.PR, p53^UAS.Ex has decreased body size | pupal stage phenotype, suppressible | partially by Erk7^HMS00222, Scer\GAL4^Ilp2.PR

(Hasygar and Hietakangas, 2014)

Scer\GAL4^Ilp2.PR, p53^UAS.Ex has abnormal developmental rate phenotype, suppressible | partially by Erk7^HMS00222, Scer\GAL4^Ilp2.PR

(Hasygar and Hietakangas, 2014)

Scer\GAL4^GMR.PU, p53^UAS.Ex has visible phenotype, suppressible by Set^UAS.cKa/Scer\GAL4^GMR.PU

(Kim et al., 2012)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PU, p53^UAS.Ex has abnormal size | adult stage phenotype, non-suppressible by Glyat^c02982/Glyat[+]

(Ren et al., 2017)

Scer\GAL4^GMR.PU, p53^UAS.Ex has visible | adult stage phenotype, non-suppressible by Glyat^c02982/Glyat[+]

(Ren et al., 2017)

Scer\GAL4^GMR.PU, p53^UAS.Ex has abnormal size | adult stage phenotype, non-suppressible by Glyat^TH00482, Scer\GAL4^GMR.PU

(Ren et al., 2017)

Scer\GAL4^GMR.PU, p53^UAS.Ex has visible | adult stage phenotype, non-suppressible by Glyat^TH00482, Scer\GAL4^GMR.PU

(Ren et al., 2017)

Scer\GAL4^GMR.PF, p53^UAS.Ex has increased cell death phenotype, non-suppressible by Df(3L)H99/Df(3L)XR38

(Peterson et al., 2002)

Scer\GAL4^GMR.PF, p53^UAS.Ex has visible phenotype, non-suppressible by Df(3L)H99/Df(3L)XR38

(Peterson et al., 2002)

NOT Enhancer of

Statement

Reference

p53^UAS.Ex/Scer\GAL4^ey.PH is a non-enhancer of increased cell death | larval stage | dominant phenotype of L²

(Singh et al., 2006)

Other

Statement

Reference

Scer\GAL4^insc-Mz1407, numb^TS4D.UAS, p53^UAS.Ex has increased cell death phenotype

(Ouyang et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PU, p53^UAS.Ex has eye phenotype, enhanceable by Hr3^RA.UAS, Scer\GAL4^GMR.PU

(Kim et al., 2011)

Scer\GAL4^GMR.PU, p53^UAS.Ex has eye phenotype, enhanceable by Hr3^RB.UAS, Scer\GAL4^GMR.PU

(Kim et al., 2011)

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, p53^UAS.Ex has eye phenotype, non-enhanceable by Su(var)2-10⁰³⁶⁹⁷/Su(var)2-10[+]

(Pardi et al., 2011)

Scer\GAL4^GMR.PF, p53^UAS.Ex has eye phenotype, non-enhanceable by lwr⁰⁵⁴⁸⁶/lwr[+]

(Pardi et al., 2011)

Scer\GAL4^GMR.PF, p53^UAS.Ex has eye phenotype, non-enhanceable by Sumo^k06307/smt3[+]

(Pardi et al., 2011)

Scer\GAL4^GMR.PF, p53^UAS.Ex has eye phenotype, non-enhanceable by lwr⁰⁵⁴⁸⁶/Sumo^k06307/smt3[+]/lwr[+]

(Pardi et al., 2011)

Scer\GAL4^GMR.PF, p53^UAS.Ex has eye phenotype, non-enhanceable by Scer\GAL4^GMR.PF/Sumo^UAS.cPa

(Pardi et al., 2011)

Scer\GAL4^GMR.PF, p53^UAS.Ex has eye phenotype, non-enhanceable by Df(3L)H99/Df(3L)XR38

(Peterson et al., 2002)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PU, p53^UAS.Ex has eye phenotype, suppressible by Set^UAS.cKa/Scer\GAL4^GMR.PU

(Kim et al., 2012)

Scer\GAL4^insc-Mz1407, p53^UAS.Ex has neuroblast phenotype, suppressible | partially by Scer\GAL4^insc-Mz1407, ago^ΔF.UAS

(Ouyang et al., 2011)

Scer\GAL4^insc-Mz1407, p53^UAS.Ex has neuroblast phenotype, suppressible | partially by CycE^UAS.cUa, Scer\GAL4^insc-Mz1407

(Ouyang et al., 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PU, p53^UAS.Ex has eye phenotype, non-suppressible by Glyat^c02982/Glyat[+]

(Ren et al., 2017)

Scer\GAL4^GMR.PU, p53^UAS.Ex has eye phenotype, non-suppressible by Glyat^TH00482, Scer\GAL4^GMR.PU

(Ren et al., 2017)

Scer\GAL4^insc-Mz1407, p53^UAS.Ex has neuroblast phenotype, non-suppressible by Scer\GAL4^insc-Mz1407/BacA\p35^UAS.cHa

(Ouyang et al., 2011)

Scer\GAL4^insc-Mz1407, numb^TS4D.UAS, p53^UAS.Ex has neuroblast phenotype, non-suppressible by dap^unspecified

(Ouyang et al., 2011)

Scer\GAL4^insc-Mz1407, numb^TS4D.UAS, p53^UAS.Ex has neuroblast phenotype, non-suppressible by BacA\p35^UAS.cHa, Scer\GAL4^insc-Mz1407

(Ouyang et al., 2011)

Scer\GAL4^GMR.PF, p53^UAS.Ex has eye phenotype, non-suppressible by Scer\GAL4^GMR.PF/Sumo^UAS.cPa

(Pardi et al., 2011)

Scer\GAL4^GMR.PF, p53^UAS.Ex has eye phenotype, non-suppressible by Su(var)2-10⁰³⁶⁹⁷/Su(var)2-10[+]

(Pardi et al., 2011)

Scer\GAL4^GMR.PF, p53^UAS.Ex has eye phenotype, non-suppressible by lwr⁰⁵⁴⁸⁶/lwr[+]

(Pardi et al., 2011)

Scer\GAL4^GMR.PF, p53^UAS.Ex has eye phenotype, non-suppressible by Sumo^k06307/smt3[+]

(Pardi et al., 2011)

Scer\GAL4^GMR.PF, p53^UAS.Ex has eye phenotype, non-suppressible by lwr⁰⁵⁴⁸⁶/Sumo^k06307/smt3[+]/lwr[+]

(Pardi et al., 2011)

Scer\GAL4^GMR.PF, p53^UAS.Ex has eye phenotype, non-suppressible by Df(3L)H99/Df(3L)XR38

(Peterson et al., 2002)

NOT Enhancer of

Statement

Reference

p53^UAS.Ex/Scer\GAL4^ey.PH is a non-enhancer of eye disc | ventral phenotype of L²

(Singh et al., 2006)

p53^UAS.Ex/Scer\GAL4^ey.PH is a non-enhancer of eye | ventral phenotype of L²

(Singh et al., 2006)

Suppressor of

Statement

Reference

Scer\GAL4^insc-Mz1407/p53^UAS.Ex is a suppressor of neuroblast | increased number phenotype of Scer\GAL4^insc-Mz1407, numb^TS4D.UAS

(Ouyang et al., 2011)

Scer\GAL4^insc-Mz1407/p53^UAS.Ex is a suppressor of neuroblast | increased number | somatic clone phenotype of numb¹⁵

(Ouyang et al., 2011)

Scer\GAL4^{VP16.nanos.UTR}/p53^UAS.Ex is a suppressor | partially of primordial germ cell phenotype of out²

(Yamada et al., 2008)

NOT Suppressor of

Statement

Reference

Scer\GAL4^insc-Mz1407, p53^UAS.Ex, CycE^UAS.cUa is a non-suppressor of neuroblast | increased number | somatic clone phenotype of numb¹⁵

(Ouyang et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The small eye phenotype resulting from the expression of p53^Scer\UAS.Ex under the control of Scer\GAL4^GMR.PU is not suppressed by heterozygosity for Glyat^c02982 or by the co-expression of Glyat^TH00482.

(Ren et al., 2017)

A Cdk7^S164A.T170A mutant background significantly suppresses the partial eye ablation found upon expression of p53^Scer\UAS.Ex in the developing eye, under the control of Scer\GAL4^GMR.PF.

(Morishita et al., 2013)

Co-expression of Set^Scer\UAS.cKa significantly suppresses the eye defects caused by expression of p53^Scer\UAS.Ex under the control of Scer\GAL4^GMR.PU.

(Kim et al., 2012)

Co-expression of Hr46^RA.Scer\UAS or Hr46^RB.Scer\UAS enhances the degenerative eye phenotype caused by Scer\GAL4^GMR.PU-mediated expression of p53^Scer\UAS.Ex.

Co-expression of Hr46^RA.Scer\UAS or Hr46^RB.Scer\UAS results in highly increased cell death in eye discs compared to Scer\GAL4^GMR.PU-mediated expression of p53^Scer\UAS.Ex alone.

(Kim et al., 2011)

Expression of p53^Scer\UAS.Ex in numb¹⁵ mutant clones under the control of Scer\GAL4^insc-Mz1407 effectively inhibits ectopic neuroblast formation.

Co-expression of p53^Scer\UAS.Ex suppresses ectopic neuroblast formation induced by numb^{TS4D.Scer\UAS} expression under the control of Scer\GAL4^insc-Mz1407.

Larval brains expressing numb^{TS4D.Scer\UAS} and p53^Scer\UAS.Ex under the control of Scer\GAL4^insc-Mz1407 exhibit an overall increase in apoptosis compared to controls (as visualised by TUNEL). However, neuroblast apoptosis remains rare, indicating that the apoptosis increase is in post-mitotic neurons or other brain cells.

A dap^unspecified mutant background does not attenuate the ability of p53^Scer\UAS.Ex expression to suppress the formation of ectopic neuroblasts induced by numb^{TS4D.Scer\UAS} expression (under the control of Scer\GAL4^insc-Mz1407).

Co-expression of ago^ΔF.Scer\UAS or CycE^Scer\UAS.cUa can partially suppress the neuroblast-loss phenotype induced by overexpression of p53^Scer\UAS.Ex under the control of Scer\GAL4^insc-Mz1407.

Co-expression of CycE^Scer\UAS.cUa and p53^Scer\UAS.Ex generates a significant increase in ectopic neuroblasts in a numb¹⁵ mutant background, compared to overexpression of p53^Scer\UAS.Ex alone in a numb¹⁵ mutant background.

(Ouyang et al., 2011)

The rough eye phenotype found at 29[o]C in flies expressing p53^Scer\UAS.Ex under the control of Scer\GAL4^GMR.PF is not affected by a Su(var)2-10⁰³⁶⁹⁷, lwr⁰⁵⁴⁸⁶, or smt3^k06307 heterozygous background nor in a lwr⁰⁵⁴⁸⁶-smt3^k06307 transheterozygous background.

Co-expression of smt3^Scer\UAS.cPa with p53^Scer\UAS.Ex in the developing eye at 25[o]C or 29[o]C, under the control of Scer\GAL4^GMR.PF does not affect the p53^Scer\UAS.Ex-related rough eye phenotype.

(Pardi et al., 2011)

The defective programmed cell death of the primordial germ cells (PGCs) see in out¹ homozygous embryos is partially rescued by expression of p53^Scer\UAS.Ex under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16}.

(Yamada et al., 2008)

Expression of p53^Scer\UAS.Ex under the control of Scer\GAL4^ey.PH does not enhance the L²/+ mutant phenotype of ventral eye loss.

(Singh et al., 2006)

The presence of p53^Scer\UAS.Ex, under the control of Scer\GAL4^GMR.PF does not affect the reduction in eye pigmentation observed in Ada2b^d842 heterozygotes.

(Pankotai et al., 2005)

Xenogenetic Interactions

Statement

Reference

Co-expression of BacA\p35^Scer\UAS.cHa does not affect the suppression of ectopic neuroblast formation by p53^Scer\UAS.Ex in numb^{TS4D.Scer\UAS}-expressing larval brains (all transgenes under the control of Scer\GAL4^insc-Mz1407).

Co-expression of BacA\p35^Scer\UAS.cHa does not affect the suppression of ectopic neuroblast formation by p53^Scer\UAS.Ex (both transgenes under the control of Scer\GAL4^insc-Mz1407) in numb¹⁵ mutant neuroblast clones.

(Ouyang et al., 2011)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

p53^Scer\UAS.Ex

p53^UAS.Ex

Name Synonyms

Secondary FlyBase IDs

References (19)

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