FlyBase Allele Report: Dmel\Hr3[RA.UAS]

General Information

Symbol

Dmel\Hr3^RA.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0261083

Feature type

allele

Associated gene

Dmel\Hr3

Associated Insertion(s)

Carried in Construct

P{UAS-Hr3.RA}

Key Links

Transgenic product class

wild_type

(Montagne et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Montagne et al., 2010)

Mutagen

in vitro construct

(Montagne et al., 2010)

Progenitor genotype

Carried in construct

P{UAS-Hr3.RA}

Cytology

Description

UAS regulatory sequences drive expression of Hr3 coding sequences corresponding to the RA transcript.

(Montagne et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Hr3

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death | conditional, with Scer\GAL4^GMR.PU

(Kim et al., 2011)

lethal, with Scer\GAL4^ap-md544

(Montagne et al., 2010)

Phenotype Manifest In

eye disc | conditional, with Scer\GAL4^GMR.PU

(Kim et al., 2011)

Detailed Description

Statement

Reference

Scer\GAL4^GMR.PU-mediated expression of Hr46^RA.Scer\UAS results in little or no phenotype in the eye.

IR irradiation of eye discs expressing Hr46^RA.Scer\UAS under the control of Scer\GAL4^GMR.PU results in highly increased apoptosis compared to irradiation of control discs.

(Kim et al., 2011)

Expression of Hr46^RA.Scer\UAS induced by Scer\GAL4^ap-md544 leads to lethality.

(Montagne et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Hr3^RA.UAS, Scer\GAL4^GMR.PU has increased cell death | conditional phenotype, suppressible by p53^E4

(Kim et al., 2011)

Enhancer of

Statement

Reference

Hr3^RA.UAS, Scer\GAL4^GMR.PU is an enhancer of increased cell death phenotype of Scer\GAL4^GMR.PU, p53^UAS.Ex

(Kim et al., 2011)

Phenotype Manifest In

Enhancer of

Statement

Reference

Hr3^RA.UAS, Scer\GAL4^GMR.PU is an enhancer of eye phenotype of Scer\GAL4^GMR.PU, p53^UAS.Ex

(Kim et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Hr46^RA.Scer\UAS enhances the degenerative eye phenotype caused by Scer\GAL4^GMR.PU-mediated expression of p53^Scer\UAS.Ex.

Co-expression of Hr46^RA.Scer\UAS results in highly increased cell death in eye discs compared to Scer\GAL4^GMR.PU-mediated expression of p53^Scer\UAS.Ex alone.

p53^E4 prevents the IR irradiation-induced increase in apoptosis seen in eye discs expressing Hr46^RA.Scer\UAS under the control of Scer\GAL4^GMR.PU.

(Kim et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Hr3^RA.Scer\UAS

Hr3^RA.UAS

Hr46^RA.Scer\UAS

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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