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FB2026_01
,
released March 12, 2026
DHR3, Hr46, Hormone receptor-like in 46, DmHr3, Complementation group D
transcription factor - nuclear receptor superfamily - a second tier regulator involved in Drosophila molting - acts negatively on Ecdysone receptor and postively on genes expressed subsequently in the molting hierarchy
Please see the JBrowse view of Dmel\Hr3 for information on other features
To submit a correction to a gene model please use the Contact FlyBase form
AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Gene model reviewed during 5.56
Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated
Gene model reviewed during 6.13
Gene model reviewed during 5.49
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.44
Stop-codon suppression (UAA) postulated; FBrf0216884
Unconventional translation start (ACG) postulated;
9, 7, 5.5 (northern blot)
487 (aa)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Hr3 using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Comment: reported as posterior spiracle specific anlage
Comment: reference states 4 hours before puparium formation
Hr3 expression is enriched in border follicle cells relative to other cells in the egg chamber.
Hr36 transcript expression peaks coincide with ecdysteriod peaks in embryos, larvae, and pupae.
Hr3 expression was studied in staged third instar larvae and prepupae collected at two hour intervals. Hr3 transcripts can be detected at low levels in late third instar larvae (116-120hr after egg laying). Expression peaks in 2-4hr prepupae and declines significantly by 8hr after puparium formation. In staged prepupal salivary glands, Hr3 transcripts are abundant in 0-2hr prepupal glands, decrease in 4hr glands, and are undetectable at later stages. Hr3 transcripts are rapidly induced by ecdysone.
Comment: 4-6 hr APF
Hr26B protein expression is upregulated in the ring gland very late in larval development; expression levels are higher at "cleared gut" stage than at the earlier "blue gut" stage.
JBrowse - Visual display of RNA-Seq signals
View Dmel\Hr3 in JBrowse
2-62
2-60.5
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R+ cells.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
Loss of Hr46 activity is not cell lethal.
See Koelle, Seagraves and Hogness, PNAS 89:6167--6171 .
Complementation group identified in an EMS and DEB screen to isolate deficiencies that uncover Jra.
Structural similarity to the M.sexta early gene and the expression similarities to the known Drosophila early genes suggest that the Hr46 gene may also belong to the early class.
Source for merge of: Hr46 l(2)46CFi
Source for merge of: Hr46 CG12208
One or more of the processed transcripts for these genes contain several non-overlapping open reading frames (ORFs). The non-overlapping ORFs are represented by CG33183 (FBgn0000448), CG12912 (FBgn0033497) and CG46321 (FBgn0284237). Different transcripts of Hr3 (CG33138) are dicistronic for CG12912 and CG46321; no one transcript is polycistronic.
Annotations CG11823 and CG12208 merged as CG33183 in release 3 of the genome annotation.
Source for identity of: Hr3 Hr46
Renamed from 'Hr46' to 'Hr3' to reflect overwhelming usage in the literature and the symbol used in the initial paper to identify the gene (FBrf0057540).
