FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Hr3, e(y)3
FlyBase ID
FBig0000108407
Interaction Network
Interactions Browser links
Hr3 network
e(y)3 network
Reported Interactions
FBrf0213948-1.Y2H
Reference
Vorobyeva et al., 2011
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
e(y)3 

e(y)3

bait

fused to LexA DNA-binding domain

e(y)3 
Hr3 

Hr3

prey

fused to GAL4 activation domain

Hr3 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
e(y)3 
AT hook region
sufficient binding region
aa 644-889

coordinates relative to e(y)3-PA

Hr3 
ligand binding domain
sufficient binding region
aa 111-487

coordinates relative to Hr3-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Vorobyeva et al., 2011)

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Vorobyeva et al., 2011)
FBrf0213948-2.CH.WB
Reference
Vorobyeva et al., 2011
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stageorganism / pupal stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
e(y)3 

e(y)3

neutral component
e(y)3 

e(y)3

neutral component
e(y)3 

e(y)3

neutral component
Hr3 

Hr3

neutral component
Hr3 

Hr3

neutral component
Hr3 

Hr3

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of wild-type embryos or pupae of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Vorobyeva et al., 2011)
FBrf0213948-3.coIP.WB
Reference
Vorobyeva et al., 2011
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stageorganism / pupal stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Hr3 

Hr3

bait
e(y)3 

e(y)3

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of wild-type embryos or pupae of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Vorobyeva et al., 2011)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)