FlyBase Gene Report: Dmel\e(y)3

(Fisher et al., 2012)

embryonic stage 7 -- 8

anterior endoderm anlage

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

hindgut anlage

(Fisher et al., 2012)

inclusive hindgut primordium

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

ventral ectoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

(Fisher et al., 2012)

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

larval ventral nerve cord

(Fisher et al., 2012)

(Fisher et al., 2012)

larval stage

imaginal disc

(Vorobyeva et al., 2009)

adult stage | female

ovary

(Vorobyeva et al., 2009)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

organism

(Vorobyeva et al., 2009)

adult stage | female

ovary

(Vorobyeva et al., 2009)

Additional Descriptive Data

e(y)3 transcript is expressed in many tissues during Drosophila development, and is most intense in embryos, in imaginal discs, and in the adult ovary.

(Vorobyeva et al., 2009)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | female

ovary

(Vorobyeva et al., 2009)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of brahma complex

inferred from direct assay

(Barish et al., 2020, Chalkley et al., 2008)

located_in euchromatin

inferred from direct assay

(Shidlovskii et al., 2005)

located_in heterochromatin

inferred from direct assay

(Shidlovskii et al., 2005)

located_in nucleus

inferred from direct assay

(Shidlovskii et al., 2005)

inferred from direct assay

(Shidlovskii et al., 2005)

located_in polytene chromosome chromocenter

inferred from direct assay

(Shidlovskii et al., 2005)

located_in polytene chromosome

inferred from direct assay

(Nikolenko et al., 2005)

inferred from direct assay

(Shidlovskii et al., 2005)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\e(y)3 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\e(y)3

View exonic expression by tissue for Dmel\e(y)3

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0087008

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

32965/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0087008

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0087008

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG12238

Flygut - An atlas of the Drosophila adult midgut

FBgn0087008

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\e(y)3

Stages(s) 4-6
Stages(s) 7-8
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 29 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 15 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of e(y)3

Transgenic constructs containing regulatory region of e(y)3

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene duplicated in

Dp(1;3)DC364

(Popodi et al., 2010-, Venken et al., 2010)

Dp(1;3)DC365

(Popodi et al., 2010-, Venken et al., 2010)

Inferred from location ( 15 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

e(y)3^EMSl

lethal | pharate adult stage

e(y)3^G0409

lethal | pupal stage

e(y)3^G0409

lethal | recessive

lethal - all die before end of P-stage

e(y)3^G0381

lethal - all die before end of pupal stage

lethal - all die during embryonic stage | recessive

e(y)3^EMSl

partially lethal

e(y)3⁴

partially lethal | recessive

partially lethal - majority die | male limited

e(y)3⁴

partially lethal - majority live

e(y)3⁴

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

e(y)3^GD7544

viable, with Scer\GAL4^pnr-MD237

e(y)3^GD7544

viable, with Scer\GAL4^tin.CΔ4

e(y)3^GD7544

Sterility

Allele

female sterile

e(y)3⁴

female sterile | recessive

fertile

e(y)3^EY08767

male fertile, with Scer\GAL4^bam.PU

e(y)3^HMS00337

Other Phenotypes

Allele

abnormal cell cycle

e(y)3⁴

decreased body size

e(y)3⁴

non-suppressor of variegation, with P{hsp26-pt-T}118E-3

e(y)3⁴

some die during embryonic stage | recessive

e(y)3^EMSl

some die during pharate adult stage

e(y)3^G0381

some die during pupal stage

suppressor of variegation

suppressor of variegation, with In(1)w^m4h

e(y)3⁴

suppressor of variegation, with P{hsp26-pt-T}39C-12

e(y)3⁴

suppressor of variegation, with P{hsp26-pt-T}39C-42

e(y)3⁴

suppressor of variegation, with P{hsp26-pt-T}39C-52

e(y)3⁴

suppressor of variegation, with P{hsp26-pt-T}118E-10

e(y)3⁴

suppressor of variegation, with P{hsp26-pt-T}118E-19

e(y)3⁴

suppressor of variegation, with P{hsp26-pt-T}118E-25

e(y)3⁴

visible

e(y)3⁴

visible, with Scer\GAL4^pnr-MD237

e(y)3^GD7544

visible | recessive

Phenotype manifest in

Allele

adult head

e(y)3⁴

embryonic/larval midgut

e(y)3^EMSl

embryonic/larval midgut primordium

e(y)3^EMSl

embryonic head

e(y)3^EMSl

embryonic Malpighian tubule

e(y)3^EMSl

femur

e(y)3⁴

femur | male limited

e(y)3⁴

gonad | embryonic stage

e(y)3^EMSl

leg

e(y)3⁴

macrochaeta

mesonotum, with Scer\GAL4^pnr-MD237

e(y)3^GD7544

wing

e(y)3⁴

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (6)

Hsap\PHF10

8 of 14

Yes

3 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (6)

Rnor\Phf10

7 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (6)

Mmus\Phf10

8 of 14

Yes

3 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (6)

Xtro\phf10

5 of 13

Yes

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (11)

Drer\phf10

8 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (4)

Cele\phf-10

6 of 14

Yes

Cele\dpff-1

2 of 14

Cele\chd-3

1 of 14

Cele\set-26

1 of 14

Anopheles gambiae (African malaria mosquito) (10)

Agam\AgaP_AGAP001877

9 of 12

Yes

Agam\AgaP_AGAP001386

1 of 12

Agam\AgaP_AGAP004688

1 of 12

Yes

Agam\AgaP_AGAP004706

1 of 12

Yes

Agam\AgaP_AGAP004713

1 of 12

Yes

Agam\AgaP_AGAP004897

1 of 12

Yes

Agam\AgaP_AGAP005225

1 of 12

Agam\AgaP_AGAP005338

1 of 12

Yes

Agam\AgaP_AGAP010299

1 of 12

Yes

Agam\AgaP_AGAP013040

1 of 12

Yes

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:e(y)3. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (11)

3 of 13

tth

2 of 13

CG2926

1 of 13

chm

1 of 13

enok

1 of 13

Lpt

1 of 13

mof

1 of 13

Myst5

1 of 13

Tip60

1 of 13

tou

1 of 13

upSET

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Coffin-Siris syndrome (postulated), PHF10-related

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 2 )

Allele

Disease

Evidence

References

e(y)3^KK112108

model of Coffin-Siris syndrome

CEA

(Chubak et al., 2019)

e(y)3^HMS00337

model of Coffin-Siris syndrome

CEA

(Chubak et al., 2019)

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 2 )

Allele

Disease

Interaction

References

e(y)3^HMS00337

exacerbates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Feuillette et al., 2020)

ameliorates amyotrophic lateral sclerosis type 10

modeled by Hsap\TARDBP^{M.UAS.Tag:FLAG}

(Azpurua et al., 2021)

e(y)3^MI11912

exacerbates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Feuillette et al., 2020)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PHF10; PHD finger protein 10

8 of 14

613069

DPF3; double PHD fingers 3

3 of 14

601672

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

e(y)3 - Bap111

anti bait coimmunoprecipitation, Identification by mass spectrometry, western blot, anti tag coimmunoprecipitation

(Chalkley et al., 2008)

e(y)3 - Bap170

(Vorobyeva et al., 2009, Chalkley et al., 2008)

e(y)3 - Bap60

ion exchange chromatography, molecular sieving, western blot, anti bait coimmunoprecipitation, Identification by mass spectrometry

(Vorobyeva et al., 2009)

e(y)3 - Brd7-9

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Barish et al., 2020)

e(y)3 - CG3517

two hybrid

(Hu et al., 2017.6.13)

e(y)3 - CG9133

two hybrid

(Hu et al., 2017.6.13)

e(y)3 - Hr3

molecular sieving, western blot, anti bait coimmunoprecipitation, two hybrid

(Vorobyeva et al., 2011)

e(y)3 - Snr1

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, anti tag western blot, molecular sieving

(Chalkley et al., 2008)

e(y)3 - Stat92E

(Panov et al., 2012)

e(y)3 - Taf1

anti tag coimmunoprecipitation, western blot, anti bait coimmunoprecipitation, Identification by mass spectrometry, ion exchange chromatography, molecular sieving

(Vorobyeva et al., 2009, Vorobyeva et al., 2008)

e(y)3 - Taf10

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation

(Vorobyeva et al., 2009, Soshnikova et al., 2008, Vorobyeva et al., 2008)

e(y)3 - Taf13

(Vorobyeva et al., 2009, Soshnikova et al., 2008)

e(y)3 - Taf3

anti bait coimmunoprecipitation, western blot, Identification by mass spectrometry, anti tag coimmunoprecipitation

(Vorobyeva et al., 2009, Soshnikova et al., 2008)

e(y)3 - Taf4

(Vorobyeva et al., 2009, Vorobyeva et al., 2008)

e(y)3 - Taf5

anti bait coimmunoprecipitation, Identification by mass spectrometry, coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, anti tag western blot

(Vorobyeva et al., 2009)

e(y)3 - Taf8

anti bait coimmunoprecipitation, Identification by mass spectrometry, western blot

(Vorobyeva et al., 2009)

e(y)3 - Taf9

anti tag coimmunoprecipitation, western blot, ion exchange chromatography, molecular sieving, anti bait coimmunoprecipitation, Identification by mass spectrometry

(Vorobyeva et al., 2009, Vorobyeva et al., 2008)

e(y)3 - Tbp

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, ion exchange chromatography, molecular sieving

(Vorobyeva et al., 2009, Soshnikova et al., 2008, Vorobyeva et al., 2008)

e(y)3 - brm

anti bait coimmunoprecipitation, Identification by mass spectrometry, western blot, anti tag coimmunoprecipitation

(Tilly et al., 2021, Vorobyeva et al., 2009, Chalkley et al., 2008)

e(y)3 - mor

anti bait coimmunoprecipitation, western blot, Identification by mass spectrometry, anti tag coimmunoprecipitation, ion exchange chromatography, molecular sieving, coimmunoprecipitation

(Vorobyeva et al., 2009, Chalkley et al., 2008, Vorobyeva et al., 2008)

e(y)3 - polybromo

anti tag coimmunoprecipitation, western blot, anti bait coimmunoprecipitation, coimmunoprecipitation, ion exchange chromatography, molecular sieving, Identification by mass spectrometry

(Vorobyeva et al., 2009, Chalkley et al., 2008, Vorobyeva et al., 2008)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

Bap170|polybromo

(Chalkley et al., 2008)

enhanceable

brm

(Chalkley et al., 2008)

enhanceable

mor

(Chalkley et al., 2008)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

enhanceable

(Melnikova et al., 1996)

suppressible

e(y)3|Bap170

(Chalkley et al., 2008)

suppressible

(Gause et al., 1996)

enhanceable

(Georgiev, 1994)

enhanceable

(Georgiev and Gerasimova, 1989)

enhanceable

(Georgiev, 1994)

z|w

enhanceable