Comment: 0-22 hr AEL
TAGAATCGGCAGCCGCCACGTCCACAGCAGGATCAGCCGGAGCAGCAGGTCGCCCCCAGTCTAACTGTAGCGCTAATAGC AACGCCAAGTCAGTGGCAGCGAGCAGCACGAGCGAGGAGGAGCAGCGAGTTAGCTCCACGTCGTCGCCAGCCCAGCGGGA TCAGCAGCTGAATGCAGATCGGGACCGGGAACAGGAGCCAGAGCCACAGCAGCAGCAGCAGCGGGAGGAGGCACTGCAGC ACCAGCATAATCAGCCCGGCCATATAACCAGCACCACAGCGTCGCCTCCGCCGACGCTGCCACCGCCGACGACGCCGTGC GACGATGCCCCAAGCACAACTGGAGCATCTGCATCGGCCAGCTCAGCATCCGGAGAAGCACCATCAGCAGCATCAGCAGC GGGAGCAGCAGGTGGACCGATGGCCGCTACGGCGCTTGAGGTAGAGAGCGAGGAGCGGGATGGACATAAGATCATCCTGA AGCTCTCCAAGCACGCCAATCCCAACTCAAACGCAAATGAATCTCAGCCGGGCGGAGATGAGAGGCGCGTGGAGCCCCTG CGCATTCAGCTGCCCTGCGGCGGAGCGGAAGGAGGCTTGGTTGCGAAGCAGCAAGATGCCTTCGATGCGGACGCCTCTTC CTGCTCCTCGTCGTGCGCCGAGGACGAGGTGGCTACCACCCTCGGCCAACAACTACGAAACACCCCGCACATTGTGCCAA AGCTGACCATCCGCGCCGCGAACGAGAGGCGCGTAGGCAGCGTGGTGCCTAAGCTGACCATCAAGTTGCCTGAGAATCCG GCTGCTTCCGGCTCCAACTCCAACTCTGGATCGTGCTCAGCAGCTGTGAGTGGCGCCCAGTCAGCTATGCCCGCCAAGAA CGATGCCCACCTGTCCAGCCTCTCGCCCGCATCCGCCTCGTCTTCATCTGCCTCGTCCTCCTCCTCATCGTCGTCGTCGT CCTCACTGGCGGAGATGCAGACGGTGCCCAAGCTGATGATCAAGACAACGCTAGCCGGTAGTAGCTGCATCAGCAGCAGT GAGGAGCTTCCGCAGCAGCAGCAGCAGATACCAAAGTTAACAATCAAGACAGGTGGCGGCGGTCAGGAGCACGTGCACAC GGTCATAATGACGCACGACCTAAACAACGCCCAGAGCATCCCCAAACTCACCATTAAAACCAAATCCATAGAGATGATCG AGGACGAACAGGCGGCTAAGCTGGAGCAGCAATTGCAGCCGCTGCCCAAGCTGACCATCAAGAACTTATGCTCACCGAAG CACAAGGTGCGTGCAGTGCTCGAGGAGAAGCCACCAACGGCGGCCTCCAAGCTGGCTATCCCAAAACCCACGCCCAATCC CACGCCGGCTCCCATGACGAATGGCGGCGAGTCGAACAGCAGTAGTCAGGAATTCTGCGGCTTCTCCGACCCAGACGCAG ATATAGCTGCGCCAGCCTCGGACGACGTTCGCCGCAATTCGGACGACATGGTGATCGACGATTCGCTCTCCAAGGAGCAC GATCCCAAGATTTTTCACAATCTACCGCCCATGCCCGCCAGCAACGGCATAGCCAGCGGTGGGAGTAAGGCCAGTAAAGC CAGCAAGTCTGCCCAATCCCAGCACAACGTTGTGGACATGGTGGACCTGACCTCGTCACCGTCGCCGGGCTCTTCGCCAA CGCATGTGCCTAACAACTTCACGGGACGCATTTCGCCGAAAGGGTTGCTCATCGACTGCCTGCGGATGCAAACTGCGATC GGCTGTTATGTGCCCGAGGAGGCCGACAGTCGCGTTAGGCCAGCTGCGTCGCCCAATTCGAACATATTGCTTAGCCAGCT GACGGCACCGGCTAAAAGCTTCACAACGACCACGCCTAAAAGCAAATCCAGCAAGTATCCCCAGCTAACGGAGCGCCTGA TGGCGAATGGAAGCGGTACCGGTGGGGGATCTGTTATTGCTGCAGAAAGCGAGGCAGTCGTTGGTCCCTTGCCCGCCGCG AATCCCGCCCAATCCATAATCGAGTCCATTGAAATCCTGGACACGCCTGATGGCAGCCCGAGGGTAGCCTACATGGACGA GGATTCTAACCCGATGCTCAACAAACATCTCCAAACTATTCACAAGCTCGCAATGGATCACGGAGTGGAACAGCGTATGG ATACCCAGGACAACAACAACGAGAACCACTTGAAACGCACTAACAGCGAGGGAAACGAATCTCCCAGCAGTAGACTCCCT CCCAGCAAACAGCGACGTCTGGACAACGACGAGAACGACCAGCAGACGCAGAATTGCCACGATCCCGCCAGAAAGTGCAG TGAATCACTGCAGGCGCTTCCGCCGTCACATCGCTCGCGCAAGCAGAAGCACGAGCGAATCCTGAACACAGATCTGGAGG GTTCGCTTTTTCCACCCACCCAACAGCAGTCGATTTCAACGCCGGATCAAAATGGAGCCTTGTCCTCGGAGGTCGAAGGC GAGGACGCAAAGGCGCAAGATCCGCTAGAACCGGCGACGCCTCAGCCCCCACCGGTAGCGACGCCGGCAGGAACGGGCAA GAAGCGTGGGCGGCCGAAGCGTATCCAAAACCAGAGCTCGCCGGATGGTGGAGGTGCAGTTACTCCCAAACCCGGCACTC CACAGGAGGAGGCCAACAGCGCTGTCAAGTCGCGTCGCGTACAACTGCTGCGCAAGCGACTGGCCATCGACATGGTGAGC GTGGGCCAAGAGCAGGCGGACATGAAAGCTAAGGAAAAGGAGTCGTCTGTGGGCGTGCCAAATGCAAGAGTTGAAGACGG GCTTGCCGAAACTCTGGAATCTCCAAAGACTCGCGACCATCGCCCCCTGAGAGCCACGCGCCGCACCACGACCAGCACAA ACACCAACCTGCAGCCCACGCCCAAGTCCACAAGGAAGCGCCAGAGCAAGGCCAGTGTCCAACAAAGCCAGTTGCCGCCG CCGACGATGGCTCAGTTCGGTAGTTCGGAGTCCGAGTCCAACAACAACAATAATAGCAGCATATCCTTTGCCCTGAGCGC GCAGATCGACCTCACAATGTGCTCGTCCAGTTCGTCGACCTCCTCTGGCGCTGCCGCCAACCAGCAGGTGATCGGTGGAA GTGGCAGCAGCTCGATGCTACCTCCGACGACGATTCTGTCTTCTTCGGATCCGCTGCCCGATGTCATCTTCCAGCCAAAT GACTTCTCCTCTATCATGGCCACCCAGCAGCTGCGCTCCTCGCGCCCCTCAAGCATCAGCTGTGGCAGTACCGGCGGTAG CCAGCCGGACTGTCACGACGAGGACAACTACACCAGCGCGCTGGACAATTCCGGAGACGAAACTGCGTTGCCTATGCCCC CCACGCGGGGACGTGGTCGTGGCCGGCGATCCAGGGGAGGCCGCGGTAGAGGATCTTCTTCGGTGGATCGGGCTGTCAGC GTCGGAGGCACAGCCTCCACAACGCCCGCCACGCAGCCTAGGGCCCCGCGAATGAGCCGAGGAGCAAGTGCCGTAGCAAA GGCCATCGCCATGAGTCGACCACGATGCGTGGGCGGCCTCAAGCACCAGCCCGATCCCGAGAGACTTAAAGGCTTGTTCA GTCCGTCGCCGCAAGTGTTTGAGGAGGACACGCGTATGAGCGCCGATCTTAGCAACAGCAATCAGTCGATGGCCAGCCTG ATGGAACCCCCGCTAACGCCACAGAAGCAACCCGACTTCCTCAACAACGAGGAGTCACAGTCGTCCATGGTGAGCAATGT GAGCATGATGGACTCCAACCAGGGTCAGTCCGCCGACGCGATTCTGGGTTCTGCTCTCAAACGTCCAAAGAAGAAGAAGA TGGAAACTTGCGTGGCCGAAGACACCGACTACAGCGCCTCCAGCATCGCCGAGTACGACTGGCCACCGCCTAAAGGTTGC TGCCCATCAAAGAATCGGGACACCTTCATGATTCAAGAGCAGGTGGCGCTCTATCTGGGCATTACCAGCTTTAAGCGCAA GTACCCGGATCTGCCACGCCGATCGGTCGACATGGAGGAGCGCAATTGGCTGCAGGAGAAGGGGCTGGTTAGCGAGAAGT TGTGCGACCTGGGCATCACCGCTGTGTGGGCCTCCGACATATTGGACATCATGTACGCGGACTTTTTCGACAAGTACGAA GAGTACAAGGAGTACATTCGCAGAAAGCATTTGCGCGAGATCGAGGCTAAGCAGAAGGCCCTAGGACTCACAGTCGGGGC CGGACGAGGTCTGCAAGCTCGCGACCGTGCCATGCTTTCGGCCACCAAGTGGAACGCTTACTTCAACAAGTCCCGTAAGG ATGAGCGCCAGTCCTGCATGGACCTGCAGACCTTCACGATAAATCAGCCGCAGCCAAGGACCGCGCCCACCTGTACACGC CTAGAGCGCTCCACTTCGGATTTGATACGGGCAGTGGCAGAAGAGGCGAATATCCCGGCACCGCCTAATCTCCTGCCACC CCGCGACTATGACGAGGCCTTCCGCAACTCAGACTACGCTTATCCACTCACGGTGGTACCGGACCAGTTCTCAATGGCCT ACCGCCAGTTTGAGCCCGCCGAGCTCAGAGCCTACCCACTGGACACGGCGCTGGACAAGCCGCCGACCGATCTGATGGCT CAGCTGCTACAGGCAAAATCCGAAGCTGTAGGCAGCGATGAAATCAAAACGTCCGCCCCGGCCCCCAAGGATCTGGGGCA AGAGCAGTCCGCCATCAAATCCGTAACGGTAACGGCGCCCGTCCGACGCAGCAGAAGGTCCACACGCCAGCAGACGGACA AGGTTCGCACGGCTAGCAGCTCCAGCACGTCCTCCGCTCAATCGGTCTCATCCGCGTCCAGCGGCAACGGGAGCAGCAGT GATACGGAAAGCGGCGATGAAAGCGACTTTAGCAGCACATCGAGCTGCAGCAGTTCGACGGGTGCCTCAAGTGGAGCAGG CAGCGAGGACGAGGATGGGAACGAGTGCTCATCGTCGGTCAGGCTGTCCACCTGCGGCGTCTGTCTGCGCAGTCAGCATC GCAATGCTAGGGACATGCCGGAGGCTTTCATCCGCTGTTACACATGCCGAAAGCGCGTCCATCCCAGCTGCGTTGATATG CCACCGCGCATGGTGGGTCGCGTGAGGAACTACAATTGGCAGTGCGCCGGGTGCAAATGCTGCATCAAGTGCCGTAGCAG CCAACGTCCAGGAAAGATGCTCTATTGCGAGCAGTGCGACCGAGGCTACCACATCTACTGCTTGGGCCTCAGGACAGTCC CAGATGGGCGCTGGAGCTGTGAGCGCTGCTGTTTCTGCATGCGATGCGGGGCCACCAAGCCGGAGGGTCTGCCCCAGGTG GCCGCTCTGTCCCAGGCGTCCGGAGGACCGTCGGCCAACGGGGATCGCTCAAAGGCAGCGCGCAATAAGCGCTTGAAATG GGTGCACGAGTACCGCATCGATCATGTGACGAAGATCCGGGAGCACGCCGCCATGTTCTGCGTACCATGTGCGCGAAACA AGCCCGCCAAGCGGCAGTCGGCGGCGGGTGCGGCCGGTGCGGCAGCAGTTACTCCTGTCTTGGAGGCCACTTCCGCGCAA ACGGATGACAGTCCGATGCCCAGTCCCGGCCTGACAACCAACGGAGGACGCGCCCTTTCGCCAACGGCGGCACTGAGTCC AAAGGCAGCTGTGCCCGTGGCATCGCTGCCGCCCGTTCTTGAAGCGACGACGGTGACCACAAATATTGCGGGAACGATAG GCAGACGGCAGGCGGGTAATGCTGTCAATATTACAACGATGCAGTGCAGCAGCAGCAGCAGCAGCAACTTCAGCGGGAAC GGAGTCACCGAGGACGCGGCGAATGTCACTGCAACGGGAACGGCTACGGCGGCCGCAGGAGCACCCGCTGCCACTCCAAT AGGCATAGCTCCGCCGCCCGTTGTTGCCTGAGTGGGGGTCATACGTTATTGTGCCAAGCGTACTCTAACGGACTTATAGT AAGCGCCGGACGCGCCGCCCCTTTTACCAAGCCGCCTCCAAAGTGGTCGTAACCACAGAGCACAGCCGCCATCCCAAAAA AAGAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from 0-22-hour embryos.
Embryos at 0-22 hr AEL were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA was poly(A)+ selected twice. cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones LD01001-LD21096, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.
