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FB2026_01
,
released March 12, 2026
dHNF4, dHNF-4, NHF4, HNF-4(D), HNF4α
zinc finger transcription factor - mutants show defects in midgut, Malpighian tubule and salivary gland development - activated by fatty acids released from triglycerides, inducing enzymes that drive fatty acid oxidation for energy production
Please see the JBrowse view of Dmel\Hnf4 for information on other features
To submit a correction to a gene model please use the Contact FlyBase form
AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Gene model reviewed during 5.55
Gene model reviewed during 5.48
666 (aa)
Homodimer.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Hnf4 using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Comment: induced after emergence
Hnf4 expression is induced after emergence and peaks at day 2.
Hnf4 is expressed at high levels in oenocytes, with low levels of expression in the neighboring adult adipose tissue.
Hnf4 protein is expressed in tissues that control metabolic homeostasis. In larvae, Hnf4 is most highly expressed in the midgut and attached gastric caecae, the fat body, the Malpighian tubules, and in oenocytes. Low levels of Hnf4 protein can be detected in the proventriculus and salivary glands. Hnf4 protein is localized to the nucleus.
JBrowse - Visual display of RNA-Seq signals
View Dmel\Hnf4 in JBrowse
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
polyclonal
Starved Hnf4 mutant larvae consume glycogen normally but retain lipids in their midgut nd fat body and have increased levels of long-chain fatty acids, suggesting that they are unable to efficiently mobilize stored fat for energy.
Both triglycerides and long chain fatty acids accumulate upon starvation in Hnf4 mutant larvae.
Identified on basis of DNA sequence similarity to rat hepatocyte nuclear factor 4. The analogous expression patterns of the two genes, together with similar findings for rat hepatocyte nuclear factor 3 and fkh, suggest evolutionary relationships between a group of genes involved in gut formation in invertebrates and mammals.
Source for merge of: Hnf4 CG9310
