[29D5-29D5];[29F3-29F3];
A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
29D5;29F3
Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.
Presence of P+PBac{XP5.RB3}BSC202 was verified using the PCR methods and primers described in FBrf0175003 with the substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods.
The cytological breakpoints of Df(2L)BSC202 predicted from the progenitor P{XP}d03596 and PBac{RB}e00922 insertion sites using Release 4 coordinates are 29D5;29F3.