FlyBase Allele Report: Scer\GAL4[mex1.2.1]

General Information

Symbol

Scer\GAL4^mex1.2.1

Species

S. cerevisiae

Name

FlyBase ID

FBal0244042

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{mex1-GAL4.2.1}

Also Known As

Mex-Gal4, mex1-Gal4

Allele class

Mutagen

in vitro construct - regulatory fusion

Nature of the Allele

Allele class

Mutagen

in vitro construct - regulatory fusion

(Phillips and Thomas, 2006)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Carried in construct

P{mex1-GAL4.2.1}

Cytology

Nature of the lesion

Statement

Reference

A 2.1kb promoter fragment from the mex1 locus is fused upstream of sequence encoding a GAL4 driver.

(Phillips and Thomas, 2006)

Allele components

Product class / Tool use(s)

Regulatory region(s)

mex1

Encoded product / tool

GAL4

binary expression system - driver

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

embryonic/larval midgut

(Sieber and Thummel, 2012, Tennessen et al., 2011, Mazock et al., 2010)

embryonic/larval midgut enterocyte

(Redhai et al., 2020)

third instar larval stage

embryonic/larval midgut

(Binks et al., 2010)

adult stage

adult midgut

(Palu and Thummel, 2016, Chen et al., 2014)

enterocyte of posterior adult midgut epithelium

(Hudry et al., 2016)

Additional Information

Statement

Reference

Drives expression in the midgut.

(FlyBase Curators, 2017)

Marker for

enterocyte of posterior adult midgut epithelium

(Hudry et al., 2016)

Reflects expression of

mex1

(Palu and Thummel, 2016, Chen et al., 2014, Sieber and Thummel, 2012, Tennessen et al., 2011, Binks et al., 2010, Mazock et al., 2010)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate, with pHCl-2^KK106835

(Redhai et al., 2020)

abnormal developmental rate, with pHCl-2^NIG.11340R

(Redhai et al., 2020)

abnormal oxidative stress response, with Psn^KK109329

(Southon et al., 2013)

chemical resistant, with Psn^KK109329

(Southon et al., 2013)

chemical sensitive, with ATP7^UAS.Tag:FLAG

(Southon et al., 2013)

hyperplasia, with key^GD1249

(Chen et al., 2014)

increased cell number | adult stage, with cbt^UAS.cMa

(Mukherjee et al., 2021)

increased cell number | adult stage, with mir-958^{sponge.V2.UAS.mCherry}

(Mukherjee et al., 2021)

lethal | chemical conditional, with ATP7^UAS.Tag:FLAG

(Zhang et al., 2020)

lethal | larval stage, with Syx5^GD1743

(Norgate et al., 2010)

lethal | P-stage, with Syx5^GD1743

(Norgate et al., 2010)

lethal - all die before end of larval stage, with β-Spec^{UAS.cMa.Tag:MYC}

(Mazock et al., 2010)

long lived | female, with snoRNA:Ψ28S-1153^UAS.cSa

(Soulé et al., 2020)

partially lethal, with pHCl-2^KK106835

(Redhai et al., 2020)

some die during larval stage, with pHCl-2^KK106835

(Redhai et al., 2020)

some die during larval stage, with β-Spec^{UAS.cMa.Tag:MYC}

(Mazock et al., 2010)

viable, with Psn^KK109329

(Southon et al., 2013)

viable | larval stage, with Ae2^GD2397

(Dubreuil et al., 2010)

Phenotype Manifest In

adult gut | female, with snoRNA:Ψ28S-1153^UAS.cSa

(Soulé et al., 2020)

adult midgut, with key^GD1249

(Chen et al., 2014)

adult midgut epithelium, with cbt^UAS.cMa

(Mukherjee et al., 2021)

adult midgut epithelium, with mir-958^{sponge.V2.UAS.mCherry}

(Mukherjee et al., 2021)

embryonic/larval copper cell, with Gp93^NIG.5520R

(Maynard et al., 2010)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

ATP7^UAS.Tag:FLAG, Scer\GAL4^mex1.2.1 has chemical sensitive phenotype, suppressible by Psn^KK109329, Scer\GAL4^mex1.2.1

(Southon et al., 2013)

Suppressor of

Statement

Reference

Psn^KK109329, Scer\GAL4^mex1.2.1 is a suppressor of chemical sensitive phenotype of ATP7^UAS.Tag:FLAG, Scer\GAL4^mex1.2.1

(Southon et al., 2013)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^mex1.2.1/mag^UAS.cSa is a suppressor | partially of phenotype of Hr96^1.3', Hr96^1.5'

(Sieber and Thummel, 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^mex1.2.1/pHCl-2^UAS.cPa rescues pHCl-2^pTV-Cherry

(Redhai et al., 2020)

Zip89B^UAS.Tag:FLAG/Scer\GAL4^mex1.2.1 rescues Zip89B^Δ

(Richards et al., 2015)

Scer\GAL4^mex1.2.1, Hr96^UAS.cHa, Hr96^1.3' rescues Hr96^1.5'

(Sieber and Thummel, 2009)

Scer\GAL4^mex1.2.1/Hr96^UAS.cHa rescues Hr96¹

(Sieber and Thummel, 2009)

Fails to rescue

Scer\GAL4^mex1.2.1/Sdhaf3^UAS.cNa fails to rescue Sdhaf3^null

(Na et al., 2014)

Scer\GAL4^mex1.2.1/Mpc1^UAS.cBa fails to rescue Mpc1²/Mpc1¹

(Bricker et al., 2012)

Scer\GAL4^mex1.2.1, ERR^UAS.cTa, ERR² fails to rescue ERR¹

(Tennessen et al., 2011)

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

91368

w¹¹¹⁸; P{mex1-GAL4.2.1}10-8

91367

P{mex1-GAL4.2.1}9-1, y¹ w¹¹¹⁸

91369

w¹¹¹⁸; TM2, P{mex1-GAL4.2.1}12-8/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Scer\GAL4^mex1.2.1

Name Synonyms

Secondary FlyBase IDs

References (37)

Report Sections

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