FlyBase Allele Report: Dmel\Plc21C[GD11359]

General Information

Symbol

Dmel\Plc21C^GD11359

Species

D. melanogaster

Name

FlyBase ID

FBal0208093

Feature type

allele

Associated gene

Dmel\Plc21C

Associated Insertion(s)

Carried in Construct

P{GD11359}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD11359}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD11359

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of obesity

CEA

(Baumbach et al., 2014)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates familial hemiplegic migraine 1

modeled by cac^{RQ,SL.UAS.EGFP}

(Brusich et al., 2018)

ameliorates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Al-Ramahi et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult midgut region R3, with Scer\GAL4^mex1.2.1

(Vonolfen et al., 2024)

adult olfactory receptor neuron Or59b, with Scer\GAL4^{Orco.2.642.Hsim\VP22}

(Kain et al., 2008)

embryonic/larval neuromuscular junction | larval stage | male limited, with Scer\GAL4^elav-C155

(Brusich et al., 2018)

lipid droplet | adult stage, with Scer\GAL4^mex1.2.1

(Vonolfen et al., 2024)

Detailed Description

Statement

Reference

The expression of Plc21C^GD11359 under the control of Scer\GAL4^elav-C155 induces a significant decrease in larval NMJ mEPSP frequency only in males, and no changes in mEPSP amplitude in both males and females, as compared to controls.

(Brusich et al., 2018)

Expression of Plc21C^GD11359 driven by Scer\GAL4^phm.PO leads to developmental delay/arrest at the larval stage. Prothoracic gland cells of third instar Scer\GAL4^phm.PO>Plc21C^GD11359 larvae show significant decreases in both macro and micro calcium spikes.

(Yamanaka et al., 2015)

Flies expressing Plc21C^GD11359 in LN[[v]] neurons under the control of Scer\GAL4^P0.5.Pdf exhibit a longer rhythm period (25.1hr) compared to controls.

(Dahdal et al., 2010)

Adults expressing Plc21C^GD11359 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

The electroantennograms of animals expressing Plc21C^GD11359 under the control of either Scer\GAL4^{Or83b.2.642.T:Hsim\VP22} or Scer\GAL4^Gα49B.PS show a significant decrease in amplitude compared to controls in response to a number of odours (ethyl acetate, butanol, propionic acid, benzaldehyde and iso-amyl acetate).

The response of ab2 large basiconic hairs in the antenna of animals expressing Plc21C^GD11359 under the control of Scer\GAL4^{Or83b.2.642.T:Hsim\VP22} is significantly different from that of control cells; the firing frequency of the mutant ab2a neuron in response to ethyl acetate is strongly reduced compared to controls.

(Kain et al., 2008)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

11359

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Plc21C^GD11359, Scer\GAL4^P0.5.Pdf has abnormal circadian rhythm phenotype, non-suppressible by Gαo^GTP.UAS, Scer\GAL4^P0.5.Pdf

(Dahdal et al., 2010)

Enhancer of

Statement

Reference

Plc21C^GD11359, Scer\GAL4^elav-C155 is an enhancer | female limited of partially lethal - majority die phenotype of Scer\GAL4^elav-C155, cac^{RQ,SL.UAS.EGFP}

(Brusich et al., 2018)

Suppressor of

Statement

Reference

Plc21C^GD11359, Scer\GAL4^elav-C155 is a suppressor of abnormal neurophysiology | larval stage phenotype of Scer\GAL4^elav-C155, cac^{RQ,SL.UAS.EGFP}

(Brusich et al., 2018)

Plc21C^GD11359, Scer\GAL4^P0.5.Pdf is a suppressor of abnormal circadian rhythm phenotype of Gαo^GTP.UAS, Scer\GAL4^P0.5.Pdf

(Dahdal et al., 2010)

Other

Statement

Reference

GluRIIC^RNAi.UAS.cBa, Plc21C^GD11359, Scer\GAL4^C57, Scer\GAL4^elav-C155, Scer\GAL4^sca.PC has abnormal neurophysiology phenotype

(Brusich et al., 2015)

Phenotype Manifest In

Suppressor of

Statement

Reference

Plc21C^GD11359, Scer\GAL4^elav-C155 is a suppressor of embryonic/larval neuromuscular junction | larval stage phenotype of Scer\GAL4^elav-C155, cac^{RQ,SL.UAS.EGFP}

(Brusich et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

Female larvae expressing cac^{RQ,SL.UAS.EGFP} under the control of Scer\GAL4^elav-C155 exhibit a significant increase in NMJ mEPSP amplitude and frequency; the increased amplitude is suppressed by Plc21C^GD11359 co-expression.

(Brusich et al., 2018)

Expression of Plc21C^GD11359 prevents the compensatory increase in quantal content seen in response to reduced quantal size when GluRIIC^{dsRNA.Scer\UAS.cBa} is expressed using multiple pre- and post-synaptic drivers (Scer\GAL4^elav-C155, Scer\GAL4^sca.PC and Scer\GAL4^C57). Evoked neurotransmission is therefore disrupted. Muscle expression of Plc21C^GD11359 (under the control of Scer\GAL4^C57) do not prevent the compensatory increase and evoked neurotransmission is unaffected.

(Brusich et al., 2015)

Co-expression of Plc21C^GD11359 with G-oα47A^GTP.Scer\UAS in LN[[v]] neurons under the control of Scer\GAL4^P0.5.Pdf results in strong rhythms. These flies exhibit longer period rhythms (24.7hr) than control flies.

(Dahdal et al., 2010)

The electroantennograms of animals co-expressing Plc21C^GD11359 and Gα49B^{dsRNA.Scer\UAS.1f1} under the control of Scer\GAL4^{Or83b.2.642.T:Hsim\VP22} show a significant decrease in amplitude compared to controls in response to a number of odours (ethyl acetate, butanol, propionic acid, benzaldehyde and iso-amyl acetate).

(Kain et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v26557