FlyBase Allele Report: Dmel\pHCl-2[pTV-Cherry]

General Information

Symbol

Dmel\pHCl-2^pTV-Cherry

Species

D. melanogaster

Name

FlyBase ID

FBal0360341

Feature type

allele

Associated gene

Dmel\pHCl-2

Associated Insertion(s)

TI{mCherry}pHCl-2^pTV-Cherry

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

ends-out gene targeting

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Redhai et al., 2020)

Mutagen

ends-out gene targeting

(Redhai et al., 2020)

Progenitor genotype

Associated Insertion(s)

TI{mCherry}pHCl-2^pTV-Cherry

Cytology

Description

The pHCl-2 gene has been deleted and replaced with the following sequence: a single attP site and a loxP cassette which itself contains an mFRT71 cassette followed by Scer\GAL4 coding sequence and a polyadenylation signal (this GAL4 sequence is non-functional). The mFRT71 cassette contains 1: the mCherry coding region tagged with a membrane-targeting signal (Tag:Myr(Unk)) and followed by a polyadenylation signal (this is expected to be expressed under the control of the endogenous pHCl-2 regulatory sequences), 2: a w^+* marker.

(Redhai et al., 2020)

Allele components

Component

Use(s)

Inserted element

TI{mCherry}

promoter trap

Encoded product / tool

mCherry

red fluorescent protein

Also carries

attP

integrase target site

loxP

recombinase target site

mFRT71

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval midgut | larval stage

(Redhai et al., 2020)

embryonic/larval midgut interstitial cell | larval stage

(Redhai et al., 2020)

Detailed Description

Statement

Reference

pHCl-2^pTV-Cherry homozygotes exhibit a developmental delay (following re-colonization with A. pomorum or L. plantarum or under germ free conditions) and a reduced larval viability in animals raised under both normal and low yeast conditions.

pHCl-2^pTV-Cherry homozygous larvae -raised under low yeast conditions-

exhibit decreased mouth hook contractions per minute, a preference for zinc-supplemented food over time and a defective acidification of autophagic compartments in interstitial cells, and they are more translucent with less food in their intestines.

pHCl-2^pTV-Cherry homozygous larvae -raised under low yeast conditions- exhibit reduced luminal acidity in the copper cell region of midgut, enlarged volumes of both the lumen of the copper cell region and the interstitial cells, which also exhibit a reduction in the complexity of their characteristic basal infoldings.

pHCl-2^pTV-Cherry homozygous larvae exhibit increased gut bacterial titres following re-colonization with A. pomorum or L. plantarum.

(Redhai et al., 2020)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

pHCl-2^pTV-Cherry has abnormal developmental rate | recessive phenotype, non-enhanceable by RagA-B^Q61L.UAS/Scer\GAL4^pHCl-2.PR

(Redhai et al., 2020)