FlyBase Allele Report: Scer\GAL4[pHCl-2.PR]

FB2026_02 , released June 18, 2026

FB2026_02 , released June 18, 2026

Allele: Scer\GAL4^pHCl-2.PR

General Information

Symbol

Scer\GAL4^pHCl-2.PR

Species

S. cerevisiae

Name

pHCl-2 promoter construct of Remnant

FlyBase ID

FBal0326400

Feature type

allele

Associated gene

Associated Insertion(s)

Carried in Construct

M{pHCl-2-GAL4.R}

Also Known As

hodor-Gal4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Remnant et al., 2016)

Progenitor genotype

Carried in construct

M{pHCl-2-GAL4.R}

Cytology

Description

The GAL4 open reading frame is fused in-frame to the start codon of pHCl-2 within a BAC clone containing the full length genomic sequence of pHCl-2.

(Remnant et al., 2016)

Allele components

Component

Use(s)

Regulatory region(s)

Encoded product / tool

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

embryonic/larval Malpighian tubule

(Remnant et al., 2016)

embryonic/larval middle midgut

(Remnant et al., 2016)

embryonic/larval copper cell

(Remnant et al., 2016)

embryonic/larval midgut large flat cell

(Remnant et al., 2016)

larval iron accumulating cell

(Remnant et al., 2016)

adult Malpighian tubule principal cell

(Remnant et al., 2016)

embryonic/larval Malpighian tubule Type I cell

(Redhai et al., 2020)

embryonic/larval midgut interstitial cell

(Redhai et al., 2020)

embryonic/larval Malpighian tubule Type I cell

(Remnant et al., 2016)

adult midgut region R1

(Remnant et al., 2016)

adult midgut region R2

(Remnant et al., 2016)

adult midgut region R3

(Remnant et al., 2016)

Additional Information

Statement

Reference

Expression is most prevalent in third instar larvae in the middle midgut, which contains the copper cells, with lower expression in the flat cells and the iron cells. In larval and adult Malpighian tubules, expression is observed in the Type 1 (principle) cells and not in the Type II (stellate cells). In the adult midgut, expression is observed in the R2 and R3 compartments, with lower expression in the R1 compartment.

(Remnant et al., 2016)

Marker for

Reflects expression of

(Redhai et al., 2020, Remnant et al., 2016)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Scer\GAL4^pHCl-2.PR positive cells expressing shi^K44A.UAS exhibit an increase in interstitial cell volume and the width of the copper cell region of midgut in larvae raised under low yeast conditions.

(Redhai et al., 2020)

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^pHCl-2.PR, pHCl-2^KK106835 has abnormal developmental rate phenotype, enhanceable by mTor^HMS01114/Scer\GAL4^pHCl-2.PR

(Redhai et al., 2020)

Suppressed by

Statement

Reference

Scer\GAL4^pHCl-2.PR, pHCl-2^KK106835 has abnormal developmental rate phenotype, suppressible by S6k^STDETE.UAS, Scer\GAL4^pHCl-2.PR

(Redhai et al., 2020)

Enhancer of

Statement

Reference

mTor^HMS01114/Scer\GAL4^pHCl-2.PR is an enhancer of abnormal developmental rate phenotype of Scer\GAL4^pHCl-2.PR, pHCl-2^KK106835

(Redhai et al., 2020)

NOT Enhancer of

Statement

Reference

RagA-B^Q61L.UAS/Scer\GAL4^pHCl-2.PR is a non-enhancer of abnormal developmental rate | recessive phenotype of pHCl-2^pTV-Cherry

(Redhai et al., 2020)

Iml1^KK101116/Scer\GAL4^pHCl-2.PR is a non-enhancer of abnormal developmental rate | recessive phenotype of pHCl-2^pTV-Cherry

(Redhai et al., 2020)

Nprl2^KK101142/Scer\GAL4^pHCl-2.PR is a non-enhancer of abnormal developmental rate | recessive phenotype of pHCl-2^pTV-Cherry

(Redhai et al., 2020)

RagC-D^Q99L.UAS/Scer\GAL4^pHCl-2.PR is a non-enhancer of abnormal developmental rate | recessive phenotype of pHCl-2^pTV-Cherry

(Redhai et al., 2020)

RagA-B^T16N.UAS/Scer\GAL4^pHCl-2.PR is a non-enhancer of abnormal developmental rate | recessive phenotype of pHCl-2^pTV-Cherry

(Redhai et al., 2020)

RagC-D^S54N.UAS/Scer\GAL4^pHCl-2.PR is a non-enhancer of abnormal developmental rate | recessive phenotype of pHCl-2^pTV-Cherry

(Redhai et al., 2020)

Suppressor of

Statement

Reference

Rheb^UAS.cPa/Scer\GAL4^pHCl-2.PR is a suppressor of decreased rate of feeding behavior | larval stage phenotype of pHCl-2^pTV-Cherry

(Redhai et al., 2020)

S6k^STDETE.UAS, Scer\GAL4^pHCl-2.PR is a suppressor of abnormal developmental rate phenotype of Scer\GAL4^pHCl-2.PR, pHCl-2^KK106835

(Redhai et al., 2020)

Scer\GAL4^pHCl-2.PR/S6k^TE.UAS is a suppressor of abnormal developmental rate phenotype of pHCl-2^pTV-Cherry

(Redhai et al., 2020)

Rheb^UAS.cPa/Scer\GAL4^pHCl-2.PR is a suppressor of abnormal developmental rate phenotype of pHCl-2^pTV-Cherry

(Redhai et al., 2020)

NOT Suppressor of

Statement

Reference

RagA-B^Q61L.UAS/Scer\GAL4^pHCl-2.PR is a non-suppressor of abnormal developmental rate | recessive phenotype of pHCl-2^pTV-Cherry

(Redhai et al., 2020)

Iml1^KK101116/Scer\GAL4^pHCl-2.PR is a non-suppressor of abnormal developmental rate | recessive phenotype of pHCl-2^pTV-Cherry

(Redhai et al., 2020)

Nprl2^KK101142/Scer\GAL4^pHCl-2.PR is a non-suppressor of abnormal developmental rate | recessive phenotype of pHCl-2^pTV-Cherry

(Redhai et al., 2020)

RagC-D^Q99L.UAS/Scer\GAL4^pHCl-2.PR is a non-suppressor of abnormal developmental rate | recessive phenotype of pHCl-2^pTV-Cherry

(Redhai et al., 2020)

RagA-B^T16N.UAS/Scer\GAL4^pHCl-2.PR is a non-suppressor of abnormal developmental rate | recessive phenotype of pHCl-2^pTV-Cherry

(Redhai et al., 2020)

RagC-D^S54N.UAS/Scer\GAL4^pHCl-2.PR is a non-suppressor of abnormal developmental rate | recessive phenotype of pHCl-2^pTV-Cherry

(Redhai et al., 2020)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

pHCl-2^UAS.cPa/Scer\GAL4^pHCl-2.PR rescues pHCl-2^pTV-Cherry

(Redhai et al., 2020)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Scer\GAL4^pHCl-2.PR

Name Synonyms

pHCl-2 promoter construct of Remnant

Secondary FlyBase IDs

References (4)

Report Sections