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| Term | ends-out gene targeting | ID (Ontology) | FBcv:0000711 (FlyBase CV) | |||||||||
| Definition | Change of a genomic sequence via homologous recombinational repair (GO:0000724) that is induced by a double strand break on both sides of the homologous region in an engineered donor sequence. This double-strand break is usually generated by cutting at a single site adjacent to the homologous region in a circularized donor sequence. This typically generates a simple targeted replacement of the target sequence by the donor sequence. However, more complicated outcomes are possible.[ FlyBase:FBrf0167363 FlyBase:FBrf0202435 ] | |||||||||||
| Also Known As | "change induced by ends-out gene targeting" | |||||||||||
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recombination |__gene targeting by homologous recombination |__ends-out gene targeting 648 rec. |
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| Is a | gene targeting by homologous recombination | ||
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