FB2026_02 , released June 18, 2026
Allele: Dmel\Hnf4FRT
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General Information
Symbol
Dmel\Hnf4FRT
Species
D. melanogaster
Name
FlyBase ID
FBal0405768
Feature type
allele
Associated gene
Dmel\Hnf4
Associated Insertion(s)
TI{TI}Hnf4FRT
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Vonolfen et al., 2024)
gene targeting by homologous recombination
(Vonolfen et al., 2024)
Progenitor genotype
Associated Insertion(s)
TI{TI}Hnf4FRT
Cytology
Description

Two FRT sites have been inserted into the endogenous Hnf4 locus. The first is in a 5' intron and the second is downstream of the 3' end of the gene. A Disc\RFPDsRed.3xP3.cUa reporter has also been inserted, downstream of the stop codon but upstream of the second FRT site. This results in a conditional allele; FLP recombinase can be used to excise the artificial FRT cassette, excising most of the Hnf4 gene, disrupting its function. Excision of the FRT cassette also removes the Disc\RFPDsRed.3xP3.cUa reporter.

(Vonolfen et al., 2024)
Allele components
Component
Use(s)
Also carries
FRT
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Hnf4FRT
      Name Synonyms
      Secondary FlyBase IDs
        References (1)