FB2026_03 , released September 17, 2026
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Term bimolecular fluorescence complementation ID (Ontology) MI:0809 (Molecular Interactions)
Definition The bimolecular fluorescence complementation (BiFC) is an assay for determination of protein interactions and/or their location in living cells. This approach is based on complementation between two non- fluorescent fragments of a protein fluorophore such as green fluorescent protein (GFP) or its derivatives. Interactions between proteins fused to each fragment bring the fragments together resulting in the reconstitution of a fully functional flourophore that can be identified through fluorescence spectroscopy or microscopy.[ PubMed:11983170 ]
Also Known As"bifc" ; "gfp complementation" ; "green fluorescence protein complementation assay"
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biophysical
 |__fluorescence technology_________
experimental interaction detection  |
 |__protein complementation assay___|
                                    bimolecular fluorescence complementation  599 rec.
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Is a fluorescence technology
protein complementation assay
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  • "bifc" EXACT PSI-MI-short
    "gfp complementation" EXACT
    "green fluorescence protein complementation assay" EXACT
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