FlyBase Allele Report: Scer\GAL4[sns.GCN]

General Information

Symbol

Scer\GAL4^sns.GCN

Species

S. cerevisiae

Name

FlyBase ID

FBal0230429

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{sns-GAL4.GCN}

Also Known As

sns-GCN-Gal4, sns-GAL4

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Zhuang et al., 2009)

Progenitor genotype

Carried in construct

P{sns-GAL4.GCN}

Cytology

Description

A 2kb enhancer from intron 1 of sns regulates expression of a GAL4 driver.

(Zhuang et al., 2009)

Allele components

Component

Use(s)

Regulatory region(s)

sns

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 16 -- third instar larval stage

embryonic/larval garland cell

(Zhuang et al., 2009)

larval stage

larval pericardial cell

(Pendse et al., 2013)

embryonic/larval garland cell

(Pendse et al., 2013)

third instar larval stage

larval pericardial cell

(Lim et al., 2014)

adult stage

adult pericardial cell

(Na et al., 2015, Lim et al., 2014)

Additional Information

Statement

Reference

ScerGAL4^sns.GCN drives expression in nephrocytes.

(Pendse et al., 2013)

Marker for

Reflects expression of

sns

(Lim et al., 2014, Pendse et al., 2013)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal oxidative stress response, with Cat^UAS.cAa

(Lim et al., 2014)

decreased cell number | third instar larval stage, with Hnf4^{R167W.UAS.Tag:HA}

(Marchesin et al., 2019)

decreased cell number | third instar larval stage, with Hnf4^{UAS.ORF.GW.Tag:HA}

(Marchesin et al., 2019)

decreased cell size | third instar larval stage, with Hnf4^{UAS.ORF.GW.Tag:HA}

(Marchesin et al., 2019)

viable, with Hnf4^{UAS.ORF.GW.Tag:HA}

(Marchesin et al., 2019)

Phenotype Manifest In

adult heart, with Cat^UAS.cAa

(Lim et al., 2014)

adult pericardial cell, with cindr^RNAi.UAS.cUa

(Na et al., 2015)

embryonic/larval garland cell, with kirre^GD2595

(Zhuang et al., 2009)

embryonic/larval garland cell, with kirre^GD14476

(Zhuang et al., 2009)

embryonic/larval garland cell, with pyd^GD8691

(Zhuang et al., 2009)

embryonic/larval garland cell, with sns^GD65

(Zhuang et al., 2009)

embryonic/larval garland cell, with sns^RNAi.UAS.WIZ

(Zhuang et al., 2009)

embryonic/larval garland cell, with sns^UAS.Tag:HA

(Zhuang et al., 2009)

embryonic/larval garland cell | third instar larval stage, with Hnf4^JF02539

(Marchesin et al., 2019)

garland cell, with sdt^HMS01652

(Weide et al., 2017)

garland cell, with sdt^NIG.15342R

(Weide et al., 2017)

lipid droplet | third instar larval stage, with Hnf4^JF02539

(Marchesin et al., 2019)

nephrocyte | third instar larval stage, with Hnf4^{R167W.UAS.Tag:HA}

(Marchesin et al., 2019)

nephrocyte | third instar larval stage, with Hnf4^{UAS.ORF.GW.Tag:HA}

(Marchesin et al., 2019)

slit diaphragm | decreased number | larval stage, with Hsap\RAP1B^N17.UAS

(Maywald et al., 2022)

slit diaphragm | decreased number | larval stage, with Hsap\RAP1B^UAS.cMa

(Maywald et al., 2022)

slit diaphragm | decreased number | larval stage, with Hsap\RAP1B^V12.UAS

(Maywald et al., 2022)

slit diaphragm | decreased number | larval stage, with Magi^GD10066

(Maywald et al., 2022)

slit diaphragm | decreased number | larval stage, with Rap1^GD9686

(Maywald et al., 2022)

slit diaphragm | decreased number | larval stage, with Rap1^KK107785

(Maywald et al., 2022)

slit diaphragm | decreased number | larval stage, with Rap1^{N17.UAS.Tag:MYC}

(Maywald et al., 2022)

slit diaphragm | decreased number | larval stage, with Rap1^UAS.Tag:MYC

(Maywald et al., 2022)

slit diaphragm | decreased number | larval stage, with Rap1^{V12.UAS.Tag:MYC}

(Maywald et al., 2022)

slit diaphragm | decreased number | larval stage, with sns^RNAi.UAS.cUa

(Maywald et al., 2022)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^sns.GCN, sns^RNAi.UAS.cUa has slit diaphragm | larval stage | decreased number phenotype, suppressible by Rap1^UAS.Tag:MYC, Scer\GAL4^sns.GCN

(Maywald et al., 2022)

Rap1^GD9686, Scer\GAL4^sns.GCN has slit diaphragm | larval stage | decreased number phenotype, suppressible | partially by Hsap\RAP1B^UAS.cMa, Scer\GAL4^sns.GCN

(Maywald et al., 2022)

Rap1^KK107785, Scer\GAL4^sns.GCN has slit diaphragm | larval stage | decreased number phenotype, suppressible | partially by Hsap\RAP1B^UAS.cMa, Scer\GAL4^sns.GCN

(Maywald et al., 2022)

Suppressor of

Statement

Reference

Rap1^UAS.Tag:MYC, Scer\GAL4^sns.GCN is a suppressor of slit diaphragm | larval stage | decreased number phenotype of Scer\GAL4^sns.GCN, sns^RNAi.UAS.cUa

(Maywald et al., 2022)

Hsap\RAP1B^UAS.cMa, Scer\GAL4^sns.GCN is a suppressor | partially of slit diaphragm | larval stage | decreased number phenotype of Rap1^GD9686, Scer\GAL4^sns.GCN

(Maywald et al., 2022)

Hsap\RAP1B^UAS.cMa, Scer\GAL4^sns.GCN is a suppressor | partially of slit diaphragm | larval stage | decreased number phenotype of Rap1^KK107785, Scer\GAL4^sns.GCN

(Maywald et al., 2022)

Scer\GAL4^sns.GCN/Mmus\Kirrel^UAS.Tag:V5 is a suppressor of embryonic/larval garland cell | third instar larval stage phenotype of kirre^sps-1

(Helmstadter et al., 2012)

Scer\GAL4^sns.GCN/Mmus\Kirrel^UAS.Tag:V5 is a suppressor of nucleus | increased number | third instar larval stage phenotype of kirre^sps-1

(Helmstadter et al., 2012)

NOT Suppressor of

Statement

Reference

Scer\GAL4^sns.GCN/Mmus\Kirrel3^UAS.Tag:V5 is a non-suppressor of embryonic/larval garland cell | third instar larval stage phenotype of kirre^sps-1

(Helmstadter et al., 2012)

Scer\GAL4^sns.GCN/Mmus\Kirrel3^UAS.Tag:V5 is a non-suppressor of nucleus | increased number | third instar larval stage phenotype of kirre^sps-1

(Helmstadter et al., 2012)

Mmus\Kirrel2^UAS.Tag:V5/Scer\GAL4^sns.GCN is a non-suppressor of embryonic/larval garland cell | third instar larval stage phenotype of kirre^sps-1

(Helmstadter et al., 2012)

Mmus\Kirrel2^UAS.Tag:V5/Scer\GAL4^sns.GCN is a non-suppressor of nucleus | increased number | third instar larval stage phenotype of kirre^sps-1

(Helmstadter et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^sns.GCN/kirre^UAS.GFP rescues kirre^sps-1

(Tutor et al., 2014)

Scer\GAL4^sns.GCN/kirre^UAS.cRa rescues kirre^sps-1

(Helmstadter et al., 2012)

Partially rescues

Scer\GAL4^sns.GCN/kirre^{Y810A.UAS.GFP} partially rescues kirre^sps-1

(Tutor et al., 2014)

kirre^{ΔCT1.UAS.Tag:FLAG}/Scer\GAL4^sns.GCN partially rescues kirre^sps-1

(Helmstadter et al., 2012)

kirre^{ΔCT2.UAS.Tag:FLAG}/Scer\GAL4^sns.GCN partially rescues kirre^sps-1

(Helmstadter et al., 2012)

kirre^{ΔCT3.UAS.Tag:FLAG}/Scer\GAL4^sns.GCN partially rescues kirre^sps-1

(Helmstadter et al., 2012)

Fails to rescue

kirre^ext.UAS.GFP/Scer\GAL4^sns.GCN fails to rescue kirre^sps-1

(Tutor et al., 2014)

kirre^{Δ610-959.UAS.Tag:FLAG}/Scer\GAL4^sns.GCN fails to rescue kirre^sps-1

(Helmstadter et al., 2012)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

92192

y¹ w¹¹¹⁸; P{sns-GAL4.GCN}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Scer\GAL4^sns.GCN

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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