FlyBase Allele Report: Mmus\Kirrel[UAS.Tag:V5]

General Information

Symbol

Mmus\Kirrel^UAS.Tag:V5

Species

M. musculus

Name

FlyBase ID

FBal0279840

Feature type

allele

Associated gene

Mmus\Kirrel

Associated Insertion(s)

Carried in Construct

P{UAS-Neph1.V5}

Transgenic product class

wild_type

(Helmstadter et al., 2012)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Helmstadter et al., 2012)

Mutagen

in vitro construct

(Helmstadter et al., 2012)

Progenitor genotype

Carried in construct

P{UAS-Neph1.V5}

Cytology

Description

UASt regulatory sequences drive expression of a Tag:V5-tagged Mmus\Kirrel.

(Helmstadter et al., 2012)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mmus\Kirrel

Tagged with

Tag:V5

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^hs.2sev

(Helmstadter et al., 2012)

Phenotype Manifest In

adult optic lobe, with Scer\GAL4^MZ1369

(Helmstadter et al., 2012)

embryonic/larval garland cell | third instar larval stage, with Scer\GAL4^pros.PMG

(Helmstadter et al., 2012)

eye, with Scer\GAL4^hs.2sev

(Helmstadter et al., 2012)

nucleus | increased number | third instar larval stage, with Scer\GAL4^pros.PMG

(Helmstadter et al., 2012)

ommatidium, with Scer\GAL4^hs.2sev

(Helmstadter et al., 2012)

Detailed Description

Statement

Reference

Wild-type garland cell nephrocytes (GCNs) fuse to form binucleate GCNs in third instar larvae. Ectopic expression of Mmus\Kirrel^{Scer\UAS.T:SV5\V5} driven by Scer\GAL4^pros.PMG results in excessive GCN fusion resulting in multinucleate GCN cells in third instar larvae.

Overexpression of Mmus\Kirrel^{Scer\UAS.T:SV5\V5} using Scer\GAL4^hs.2sev results in a rough eye phenotype due to fusion of ommatidia.

Overexpression of Mmus\Kirrel^{Scer\UAS.T:SV5\V5} using Scer\GAL4^MZ1369 results in optic lobe abnormalities with variable penetrance.

(Helmstadter et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^sns.GCN/Mmus\Kirrel^UAS.Tag:V5 is a suppressor of embryonic/larval garland cell | third instar larval stage phenotype of kirre^sps-1

(Helmstadter et al., 2012)

Scer\GAL4^sns.GCN/Mmus\Kirrel^UAS.Tag:V5 is a suppressor of nucleus | increased number | third instar larval stage phenotype of kirre^sps-1

(Helmstadter et al., 2012)

Other

Statement

Reference

Hsap\NPHS1^UAS.cWb, Mmus\Kirrel^UAS.Tag:V5, Scer\GAL4^Ugt36A1.PK, sns^HMC05745 has nephrocyte phenotype

(Wolff et al., 2025)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The garland cell phenotype associated with kirre^sps-1 mutants is suppressed by the expression of Mmus\Kirrel^{Scer\UAS.T:SV5\V5} using Scer\GAL4^sns.GCN.

(Helmstadter et al., 2012)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Mmus\Kirrel^{Scer\UAS.T:SV5\V5}

Mmus\Kirrel^UAS.Tag:V5

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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