FlyBase Allele Report: Dmel\cindr[RNAi.UAS.cUa]

General Information

Symbol

Dmel\cindr^RNAi.UAS.cUa

Species

D. melanogaster

Name

FlyBase ID

FBal0407495

Feature type

allele

Associated gene

Dmel\cindr

Associated Insertion(s)

Carried in Construct

P{UAS-cindr.RNAi.U}

Key Links

Transgenic product class

RNAi_reagent

(FlyBase Curators, 2025-)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(FlyBase Curators, 2025-)

Mutagen

in vitro construct

(FlyBase Curators, 2025-)

Progenitor genotype

Carried in construct

P{UAS-cindr.RNAi.U}

Cytology

Description

UAS regulatory sequences drive expression of an RNAi line that targets cindr.

(FlyBase Curators, 2025-)

FlyBase curator comment: this entry represents a transgenic construct where the particular construct used cannot be determined.

(FlyBase Curators, 2025-)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

cindr

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of nephrotic syndrome

CEA

(Fu et al., 2017)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 1 )

The functional and structural defects in Drosophila nephrocytes expressing RNAi against cindr (Drosophila ortholog of CD2AP) are rescued by expression of wt version of human CDAP but not by the nephrotic syndrome-related mutant version carrying the K301M mutation.

(Fu et al., 2017)

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

short lived, with Scer\GAL4^Ugt36A1.PK

(Fu et al., 2017)

Phenotype Manifest In

adult pericardial cell, with Scer\GAL4^sns.GCN

(Na et al., 2015)

adult pericardial cell, with Scer\GAL4^Ugt36A1.PK

(Fu et al., 2017)

nephrocyte diaphragm | adult stage, with Scer\GAL4^Ugt36A1.PK

(Fu et al., 2017)

Detailed Description

Statement

Reference

Adult flies expressing cindr^TRiP.cUa under the control of Scer\GAL4^Dot.PK display nephrocyte functional defects (measured by uptake assays) and reduced adult lifespan relative to controls. On the ultrastructural level, knockdown of cindr results in fusion of discreet nephrocyte slit diaphragm units into semi-continuous wavelike form and loss of lacunar channels.

(Fu et al., 2017)

Adult flies expressing cindr^{dsRNA.Scer\UAS.cUa} under the control of Scer\GAL4^sns.GCN lack pericardial nephrocytes but show no difference in lifespan compared to wild-type controls.

(Na et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^Ugt36A1.PK, cindr^RNAi.UAS.cUa has short lived phenotype, suppressible | partially by Hsap\CD2AP^UAS.cFa, Scer\GAL4^Ugt36A1.PK

(Fu et al., 2017)

NOT suppressed by

Statement

Reference

Scer\GAL4^Ugt36A1.PK, cindr^RNAi.UAS.cUa has short lived phenotype, non-suppressible by Hsap\CD2AP^K301M.UAS, Scer\GAL4^Ugt36A1.PK

(Fu et al., 2017)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^Ugt36A1.PK, cindr^RNAi.UAS.cUa has adult pericardial cell phenotype, suppressible | partially by Hsap\CD2AP^UAS.cFa, Scer\GAL4^Ugt36A1.PK

(Fu et al., 2017)

Scer\GAL4^Ugt36A1.PK, cindr^RNAi.UAS.cUa has nephrocyte diaphragm | adult stage phenotype, suppressible | partially by Hsap\CD2AP^UAS.cFa, Scer\GAL4^Ugt36A1.PK

(Fu et al., 2017)

NOT suppressed by

Statement

Reference

Scer\GAL4^Ugt36A1.PK, cindr^RNAi.UAS.cUa has adult pericardial cell phenotype, non-suppressible by Hsap\CD2AP^K301M.UAS, Scer\GAL4^Ugt36A1.PK

(Fu et al., 2017)

Scer\GAL4^Ugt36A1.PK, cindr^RNAi.UAS.cUa has nephrocyte diaphragm | adult stage phenotype, non-suppressible by Hsap\CD2AP^K301M.UAS, Scer\GAL4^Ugt36A1.PK

(Fu et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

The nephrocyte functional defects (measured by uptake assays) as well as structural abnormalities (fusion of the slit diaphragm structures, loss of lacunar channel) and reduced adult lifespan characteristic for flies expressing cindr^TRiP.cUa under the control of Scer\GAL4^Dot.PK are significantly improved by co-expression of Hsap\CD2AP^UAS.cFa but not Hsap\CD2AP^K301M.UAS.

(Fu et al., 2017)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

cindr^RNAi.UAS.cUa

cindr^TRiP.cUa

cindr^{dsRNA.Scer\UAS.cUa}

cindr^{dsRNA.UAS.cUa}

Name Synonyms

Secondary FlyBase IDs

FBal0322786
FBal0340187

References (5)

Report Sections

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