FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Rap1N17.UAS.Tag:MYC
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General Information
Symbol
Dmel\Rap1N17.UAS.Tag:MYC
Species
D. melanogaster
Name
FlyBase ID
FBal0152268
Feature type
allele
Associated gene
Dmel\Rap1
Associated Insertion(s)
Carried in Construct
P{UAS-Rap1.N17}
Also Known As
UAS-rap1N17
Key Links
Transgenic product class
dominant_negative_variant
(Boettner et al., 2003)
missense_variant
(Boettner et al., 2003)
Mutagen
Nature of the Allele
Transgenic product class
dominant_negative_variant
(Boettner et al., 2003)
missense_variant
(Boettner et al., 2003)
Mutagen
in vitro construct
(Boettner et al., 2003)
Progenitor genotype
Carried in construct
P{UAS-Rap1.N17}
Cytology
Description

UASt regulatory sequences drive expression of a dominant negative form of Rap1 that is tagged between the 2nd and 3rd amino acid of Rap1 with Tag:MYC.

(Boettner et al., 2003)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Rap1
Tagged with
Tag:MYC
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of RN17.Scer\UAS.T:Hsap\MYC under the simultaneous control of both Scer\GAL4slbo.2.6 and Scer\GAL4upd in the border cells blocks their migration in stage 10 egg chambers.

      (Ghiglione et al., 2008)

      Embryos that express RN17.Scer\UAS.T:Hsap\MYC in the macrophages, under the control of Scer\GAL4srp, show a severe disruption in macrophage migration. Few macrophages reach the posterior region of the embryo, and a major gap remains in their distribution around the ventral nerve cord. Additionally, the macrophages were smaller and had smaller extensions than wild-type macrophages.

      (Huelsmann et al., 2006)

      No animals expressing RN17.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4ptc-559.1 hatch. 93% of embryos expressing RN17.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4ptc-559.1 have a large dorsal hole in the cuticle (covering at least half of the dorsal aspect) and the remaining 7% have a partially open cuticle. Embryos expressing RN17.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4pnr-MD237 show a strong but variable dorsal closure defect. In embryos expressing RN17.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4pnr-MD237 or Scer\GAL4ptc-559.1, the leading edge cytoskeleton is assembled largely as in wild-type embryos and an initial stretching of the lateral ectodermal cells takes place during the dorsal closure stage. However, at later stages of dorsal closure, the mutant embryos show a detachment of the lateral ectoderm from the amnioserosa; the ectoderm retracts, with the cells resuming a non-elongated shape and the amnioserosa shrivels.

      (Boettner et al., 2003)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Enhanced by
      Suppressed by
      NOT suppressed by
      Phenotype Manifest In
      NOT Enhanced by
      Suppressed by
      NOT suppressed by
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Coexpression of Gef26EP388 and RN17.Scer\UAS.T:Hsap\MYC in macrophages, under the control of Scer\GAL4srp, results in a substantial suppression of the RN17.Scer\UAS.T:Hsap\MYC macrophage phenotype. These embryos show only a minimal gap in the distribution of the macrophages around the ventral nerve cord at stage 14, and the macrophages form larger protrusions than in cells expressing only RN17.Scer\UAS.T:Hsap\MYC.

      (Huelsmann et al., 2006)

      The dorsal closure defects seen in embryos expressing RN17.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4ptc-559.1 are substantially rescued by co-expression of cnoScer\UAS.cBa. The dorsal closure defects seen in embryos expressing RN17.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4ptc-559.1 are not rescued by co-expression of cnoΔN.Scer\UAS. Co-expression of bskScer\UAS.cBa does not rescue the dorsal closure defects seen in embryos expressing RN17.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4ptc-559.1.

      (Boettner et al., 2003)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Rap1N17.UAS.Tag:MYC is rescued by Scer\GAL4ptc-559.1/Rap1UAS.Tag:MYC

      (Boettner et al., 2003)
      Comments

      The dorsal closure defects seen in embryos expressing RN17.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4ptc-559.1 are completely rescued by co-expression of RScer\UAS.T:Hsap\MYC and all the rescued animals hatch.

      (Boettner et al., 2003)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      RN17.Scer\UAS.T:Hsap\MYC
      Rap1N17.Scer\UAS.T:Hsap\MYC
      Rap1N17.UAS.Tag:MYC
      Name Synonyms
      Secondary FlyBase IDs
        References (8)