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General Information
Symbol
Dmel\bskUAS.cBa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Boutros
FlyBase ID
FBal0093136
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-bsk, UAS-JNK, UAS-basket, UASBsk, UAS-bskB
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Expression of bsk cDNA is governed by UASt regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The expression of bskScer\UAS.cBa under the control of either Scer\GAL4ptc.PU and Scer\GAL4en.PU can induce apoptosis (i.e. Casp3 expression) but is unable to cause significant cell extrusion, as compared to controls.

Neuronal expression of bskScer\UAS.cBa (under the control of Scer\GAL4elav.PU) leads to glial cell specific lipid droplet accumulation. Neuronal expression of SREBPScer\UAS.cKa alone does not cause neurodegeneration upon aging and lipid droplets are retained in aged animals.

Glial expression of bskScer\UAS.cBa (under the control of Scer\GAL454C) does not lead to lipid droplet accumulation.

Expression of bskScer\UAS.cBa under the control of Scer\GAL4twi.PG results in delayed or defective ventral furrow formation in a proportion of embryos.

Expression of bskScer\UAS.cBa under the control of Scer\GAL4Ubx-lac1-Gal4 results in transformation of posterior peripodial membrane cells of the wing disc from a hexagonal shape into an elongated shape.

Over-expression of bskScer\UAS.cBa driven in the wing by Scer\GAL4Bx-MS1096 results in ectopic vein formation in the distal portion of the 4th and 5th longitudinal veins.

Expression of bskScer\UAS.cBa under the control of Scer\GAL4Act induces autophagy in clones in the larval fat body.

Expression of bskScer\UAS.cBa in the wing, under the control of Scer\GAL4Bx-MS1096 results in mild or undetectable phenotypic changes.

Expression of bskScer\UAS.cBa under the control of Scer\GAL4ey.PH results in eyes that are smaller than wild type.

Overexpression of bskScer\UAS.cBa, under the regulation of Scer\GAL4ap-md544 has no effect on thorax closure.

Expression of bskScer\UAS.cBa under the control of Scer\GAL4GMR.PF does not affect eye development.

Flies expressing bskScer\UAS.cBa, under the control of Scer\GAL4arm.PS, show a decreased sensitivity to paraquat compared to wild-type flies.

Expression of bskScer\UAS.cBa under the control of Scer\GAL4elav-C155 has no effect on EJC amplitude (synaptic strength) and bouton number at the larval neuromuscular junction.

When expression is driven by Scer\GAL4hs.2sev, flies have rough eyes showing polarity phenotypes. Ommatidia are incorrectly rotated and sometimes exhibit the wrong chiral form.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement
Reference

bskUAS.cBa has eye phenotype, enhanceable by L2/L[+]

Suppressed by
Statement
Reference
NOT suppressed by
Enhancer of
NOT Enhancer of
Statement
Reference
Suppressor of
NOT Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

The accumulation of lipid droplets in the retinal glia of adult flies expressing bskScer\UAS.cBa under the control of Scer\GAL4nSyb.PS is significantly suppressed by co-expression of any of the following: SlnGD1940, SlnKK104306, outGD3448, outKK104187, LdhHMS00039, PdhaGD12103 or PdhaKK101856.

The reduced wing phenotype caused by expression of DLPEY09290 under the control of Scer\GAL4Bx-MS1096 is strongly exacerbated by co-expression of bskScer\UAS.cBa.

Co-expression of parkScer\UAS.T:Hsap\MYC strongly suppresses the ectopic wing veins seen with expression of bskScer\UAS.cBa driven by Scer\GAL4Bx-MS1096.

The autophagy that is induced by expression of bskScer\UAS.cBa under the control of Scer\GAL4Act in clones in the larval fat body still occurs in an Atg13Δ81 homozygous background.

Expression of bskScer\UAS.cBa under the control of Scer\GAL4tub.PU rescues both the shortened lifespan increased sensitivity to paraquat which is seen in CYLDmr4 mutant adults (animals homozygous for Df(2L)CYLDmr4 and carrying P{LX107} to rescue the function of CG13138 and nmd).

Simultaneous expression of bskScer\UAS.cBa with lkb1Scer\UAS.cLa (both under the control of Scer\GAL4Bx-MS1096 strongly enhances the lkb1Scer\UAS.cLa phenotype, resulting in severe disruption in the structure and size of the wing blades.

Expression of bskScer\UAS.cBa under the control of Scer\GAL4ey.PH enhances the loss-of-ventral-eye phenotype to near complete loss in 39% of L2/+ flies.

The thoracic cleft formation observed in parkScer\UAS.T:Hsap\MYC overexpression mutants (Scer\GAL4ap-md544) is suppressed by co-expression of bskScer\UAS.cBa.

Expression of bskScer\UAS.cBa under the control of Scer\GAL4ptc-559.1 results in a significant but partial rescue of the dorsal closure defects seen in cno2 embryos. Co-expression of bskScer\UAS.cBa does not rescue the dorsal closure defects seen in embryos expressing RN17.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4ptc-559.1.

Coexpression of bskScer\UAS.cBa with Traf1EP578, under the control of Scer\GAL4GMR.PF, leads to an increase in the disturbance of the ommatidial array in the compound eye in comparison to the eye phenotype resulting from one copy overexpression of Traf1EP578.

Coexpression of Traf2EP1516 with either bskScer\UAS.cBa, under the control of Scer\GAL4GMR.PF, does not affect the development of eyes.

The mutant dorsal appendage phenotype seen in bwk151/bwk08482 eggs is not significantly modified by expression of bskScer\UAS.cBa under the control of Scer\GAL4c415.

The proportion of eyaScer\UAS.cHa; Scer\GAL4dpp.PH flies with blistered wings (76%) is relatively unaffected by the presence of bskScer\UAS.cBa (82%), as is the proprotion with only mild wing defects 24% compared to 18%. (All data from experiments with a single, 'strong' P{UAS-eya.H} insertion; n=approximately 300 in each case).

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expressing bskScer\UAS.cBa in bskLL02244/bskLL02244 clones (with Scer\GAL4Tab2-201Y) rescues axon-pruning defects.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
bskScer\UAS.cBa
bskUAS.cBa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Boutros
Secondary FlyBase IDs
    References (33)