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General Information
Symbol
Dmel\Lkb1UAS.cLa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Lee
FlyBase ID
FBal0213123
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-LKB1WT
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of wild-type Lkb1 coding sequences derived from the full-length cDNA GM01569.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of lkb1Scer\UAS.cLa in the dorsal wing (under the control of Scer\GAL4ap-md544) results in 'bent-up' small wings.

Expression of lkb1Scer\UAS.cLa in the eye (under the control of Scer\GAL4GMR.PF) results in a small and rough eye phenotype.

Expression of lkb1Scer\UAS.cLa in the developing eye, under the control of Scer\GAL4GMR.PF does not affect neuronal differentiation.

Apoptosis induced by expression of lkb1Scer\UAS.cLa under the control of Scer\GAL4e22c, is further enhanced by butyrate treatment.

Eye-specific expression of lkb1Scer\UAS.cLa (under the control of Scer\GAL4GMR.PF) induces a slight reduction in overall eye size. This reduction becomes more severe with elevated lkb1 levels.

Dorsal tissue-specific expression of lkb1Scer\UAS.cLa (under the control of Scer\GAL4ap-md544) induces a dramatic 'bent-up' wing phenotype, in a dose-dependent manner.

Scer\GAL4Bx-MS1096-driven expression of lkb1Scer\UAS.cLa dose-dependently reduces the overall wing size.

lkb1Scer\UAS.cLa-expressing cells (generated through clonal analysis in wing imaginal discs with Scer\GAL4Scer\FRT.Act5C) expand very poorly. However, this does not cause any alterations in the bristle size.

lkb1Scer\UAS.cLa-expressing cells (generated through clonal analysis in wing imaginal discs with Scer\GAL4Bx-MS1096), while displaying a dramatic reduction in cell expansion, fail to reduce wing marginal bristle size in the adult.

Overexpression of lkb1Scer\UAS.cLa under the control of Scer\GAL4GMR.PF does not affect S-phase cell number with respect to the control tissues (as assayed with BrdU). Similarly, there is no change in the phospho-His3 staining pattern, indicating that the G2-M cel cycle transition is also unaffected. However, staining with acridine orange, which specifically labels those dying cells with disrupted membrane integrity, shows prominent death signals, compared to controls, specifically in the region of lkb1Scer\UAS.cLa. Furthermore, TUNEL staining of the eye and wing discs also confirms the existence of extensive apoptosis in the lkb1-expressing cells.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Suppressed by
Additional Comments
Genetic Interactions
Statement
Reference

Down-regulation of Sln through expression of SlndsRNA.Sym.Scer\UAS in the eye dramatically suppresses the narrow eye phenotype induced by lkb1Scer\UAS.cLa and SlnEP2245 (all under the control of Scer\GAL4GMR.PF).

Down-regulation of Sln using two copies of SlndsRNA.Sym.Scer\UAS restores the reduced wing size observed through expression of lkb1Scer\UAS.cLa (under the control of Scer\GAL4Bx-MS1096).

The fraction of SNF1AD1 mitotic larval brain neuroblasts that show extreme polyploidy is not changed if the larvae are also co-expressing lkb1Scer\UAS.cLa under the control of Scer\GAL4hs.PB.

lkb1Scer\UAS.cLa-induced apoptosis is not suppressed by expression of the dominant-negative p53Ct.GUS, both under the control of Scer\GAL4GMR.PF.

lkb1Scer\UAS.cLa-induced apoptosis is completely suppressed by expression of thScer\UAS.cHa, both under the control of Scer\GAL4GMR.PF. Interestingly, even when lkb1-induced apoptosis is completely blocked by th, the BrdU and His3 staining patterns do not deviate from the wild-type patterns.

A pucE69/+ background dramatically enhances the lkb1Scer\UAS.cLa-Scer\GAL4Bx-MS1096 small wing phenotype.

Simultaneous expression of hepAct.Scer\UAS with lkb1Scer\UAS.cLa (both under the control of Scer\GAL4Bx-MS1096 strongly enhances the lkb1Scer\UAS.cLa phenotype, resulting in severe disruption in the structure and size of the wing blades.

Simultaneous expression of bskScer\UAS.cBa with lkb1Scer\UAS.cLa (both under the control of Scer\GAL4Bx-MS1096 strongly enhances the lkb1Scer\UAS.cLa phenotype, resulting in severe disruption in the structure and size of the wing blades.

Simultaneous expression of msnScer\UAS.cSa with lkb1Scer\UAS.cLa (both under the control of Scer\GAL4Bx-MS1096 strongly enhances the lkb1Scer\UAS.cLa phenotype, resulting in severe disruption in the structure and size of the wing blades.

A hepr75 mutant background suppresses JNK pathway activation normally found when lkb1Scer\UAS.cLa is expressed by either Scer\GAL4GMR.PF or Scer\GAL4ap-md544. In addition, Scer\GAL4GMR.PF>lkb1Scer\UAS.cLa-induced apoptosis in eye imaginal discs is also completely suppressed.

Dosage reduction of grim, rpr, and W genes, through the deficiency Df(3L)H99 alleviates lkb1Scer\UAS.cLa-Scer\GAL4GMR.PF-induced apoptosis.

Xenogenetic Interactions
Statement
Reference

Co-expression of BacA\p35Scer\UAS.cHa with both lkb1Scer\UAS.cLa and SlnEP2245 considerably suppresses the apoptotic cell death induced by lkb1Scer\UAS.cLa and SlnEP2245 co-expression in the eye (all under the control of Scer\GAL4GMR.PF).

Complementation and Rescue Data
Partially rescued by
Comments

A partial downregulation of JNK activity by expression of kinase-dead lkb1K201I.Scer\UAS modestly rescues the adult phenotype induced by Scer\GAL4ap-md544-driven lkb1Scer\UAS.cLa expression.

Transgenic expression of lkb1Scer\UAS.cLa using a Scer\GAL4hs.PB driver successfully rescues the lethality of lkb1X5 mutants.

Ectopic expression of lkb1Scer\UAS.cLa under the control of Scer\GAL4hs.PB successfully suppresses the 'big brain' phenotype observed in lkb1X5 mutants.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Lkb1Scer\UAS.cLa
Lkb1UAS.cLa
lkb1Scer\UAS.cLa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Lee
Secondary FlyBase IDs
    References (5)