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General Information
Symbol
Dmel\p53Ct.GUS
Species
D. melanogaster
Name
FlyBase ID
FBal0104952
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Encodes the C-terminal fragment of p53 with defective DNA binding activity.

Construct: C-terminal fragment of p53, amino acids 282 to 385, is expressed from both GMR and Scer\UAS regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of p53Ct.GUS under the control of Scer\GAL4Bx-MS1096 has no effect on wing development.

Expression of the dominant-negative p53Ct.GUS is expressed by Scer\GAL4GMR.PF suppresses ionizing radiation-dependent apoptosis in the ninaE-expressing region.

Expression of p53Ct.GUS, under the control of Scer\GAL4elav-C155, extends lifespan by 58% in females and 32% in males. Expression of p53Ct.GUS in adult neurons, driven by Scer\GAL4elav.Switch.PO after induction with RU486, leads to a 18-26% increase in maximum lifespan in females fed on standard cornmeal food. When flies are fed on a high calorie diet, the lifespan of both males and females of this genotype is extended (by 13 and 11%, respectively). However, the lifespan of Scer\GAL4elav.Switch.PO>p53Ct.GUS flies is not increased further than the increase seen in controls when flies are fed on a low calorie diet.

The expression of p53Ct.GUS in the muscle, driven by Scer\GAL4Mhc.Switch.PO, or in the fat body, driven by either Scer\GAL4Switch1.106 or Scer\GAL4bun-Switch1.32, does not extend the lifespan of flies.

Flies that express p53Ct.GUS, under the control of Scer\GAL4elav.Switch.PO, are more resistant to paraquat poisoning than control flies. However, these flies are no more resistant to starvation or heat stress than controls. The daily egg production is not statistically different in Scer\GAL4elav.Switch.PO>p53Ct.GUS females from controls. There is also no difference in the total or average amount of spontaneous activity between Scer\GAL4elav.Switch.PO>p53Ct.GUS flies and control flies.

Co-expression of p53Ct.GUS suppresses the disruption of the eye caused by expression of p53EP-1 under the control of Scer\GAL4GMR.PF.

When expression is driven by Scer\GAL4en-e16E in the posterior compartment of the wing disc radiation-induced cell death is dramatically reduced, compared to that in the anterior compartment. There is no effect on the block of entry into mitosis upon irradiation, which occurs as it does for wild type discs. There is also no notable effect on wing pattern and size.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Enhancer of
NOT Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of p53Ct.GUS enhances the wing defects seen in flies expressing mrnGD4089 under the control of Scer\GAL4Bx-MS1096.

Co-expression of p53Ct.GUS enhances the increased apoptosis seen in the wing discs of animals expressing mrnGD4089 under the control of Scer\GAL4Bx-MS1096.

The massive apoptosis seen in wing discs co-expressing p53Ct.GUS and mrnGD4089 under the control of Scer\GAL4Bx-MS1096 is practically abolished by co-expression of bskGD10555.

Co-expression of p53Ct.GUS does not suppress the defective eye phenotype caused by expression of Sir2Scer\UAS.cGa under the control of Scer\GAL4GMR.PU.

Xenogenetic Interactions
Statement
Reference

The massive apoptosis seen in wing discs co-expressing p53Ct.GUS and mrnGD4089 under the control of Scer\GAL4Bx-MS1096 is suppressed by co-expression of BacA\p35Scer\UAS.cUa.

Co-expression of p53Ct.GUS does not rescue the autonomous reduction in wing area caused by expression of Rcom\RAcs2.Scer\UAS under the control of Scer\GAL4en-e16E, but it does rescue the non-autonomous reduction in wing area caused by expression of Rcom\RAcs2.Scer\UAS under the control of Scer\GAL4en-e16E.

lkb1Scer\UAS.cLa-induced apoptosis is not suppressed by expression of the dominant-negative p53Ct.GUS, both under the control of Scer\GAL4GMR.PF.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

Carried in a plasmid, transfected into S2 cells and used in transcription activation assays. Dominant negative mutation.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (16)