Expression of the dominant-negative p53Ct.GUS is expressed by Scer\GAL4GMR.PF suppresses ionizing radiation-dependent apoptosis in the ninaE-expressing region.
Expression of p53Ct.GUS, under the control of Scer\GAL4elav-C155, extends lifespan by 58% in females and 32% in males. Expression of p53Ct.GUS in adult neurons, driven by Scer\GAL4elav.Switch.PO after induction with RU486, leads to a 18-26% increase in maximum lifespan in females fed on standard cornmeal food. When flies are fed on a high calorie diet, the lifespan of both males and females of this genotype is extended (by 13 and 11%, respectively). However, the lifespan of Scer\GAL4elav.Switch.PO>p53Ct.GUS flies is not increased further than the increase seen in controls when flies are fed on a low calorie diet.
The expression of p53Ct.GUS in the muscle, driven by Scer\GAL4Mhc.Switch.PO, or in the fat body, driven by either Scer\GAL4Switch1.106 or Scer\GAL4bun-Switch1.32, does not extend the lifespan of flies.
Flies that express p53Ct.GUS, under the control of Scer\GAL4elav.Switch.PO, are more resistant to paraquat poisoning than control flies. However, these flies are no more resistant to starvation or heat stress than controls. The daily egg production is not statistically different in Scer\GAL4elav.Switch.PO>p53Ct.GUS females from controls. There is also no difference in the total or average amount of spontaneous activity between Scer\GAL4elav.Switch.PO>p53Ct.GUS flies and control flies.
When expression is driven by Scer\GAL4en-e16E in the posterior compartment of the wing disc radiation-induced cell death is dramatically reduced, compared to that in the anterior compartment. There is no effect on the block of entry into mitosis upon irradiation, which occurs as it does for wild type discs. There is also no notable effect on wing pattern and size.