FlyBase Allele Report: Dmel\Sirt1[UAS.cGa]

General Information

Symbol

Dmel\Sirt1^UAS.cGa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Griswold

FlyBase ID

FBal0290702

Feature type

allele

Associated gene

Dmel\Sirt1

Associated Insertion(s)

Carried in Construct

P{UAS-Sirt1.G}

Also Known As

UAS-Sir2

Key Links

Transgenic product class

wild_type

(Griswold et al., 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Griswold et al., 2008)

Mutagen

in vitro construct

(Griswold et al., 2008)

Progenitor genotype

Carried in construct

P{UAS-Sirt1.G}

Cytology

Description

UAS regulatory sequences drive expression of the Sirt1 coding region.

(Griswold et al., 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Sirt1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Parkinson's disease

modeled by Pink1^B9

(Koh et al., 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal eye color, with Scer\GAL4^GMR.PU

(Griswold et al., 2008)

abnormal feeding behavior | adult stage, with Scer\GAL4^sNPF-R.2.5

(Hong et al., 2012)

increased cell death | third instar larval stage, with Scer\GAL4^GMR.PU

(Griswold et al., 2008)

lethal, with Scer\GAL4^Act5C.PU

(Griswold et al., 2008)

lethal, with Scer\GAL4^elav.PU

(Griswold et al., 2008)

lethal, with Scer\GAL4^hs.PU

(Koh et al., 2012)

visible, with Scer\GAL4^A9

(Griswold et al., 2008)

visible, with Scer\GAL4^ey.PU

(Koh et al., 2012, Griswold et al., 2008)

visible, with Scer\GAL4^GMR.PU

(Griswold et al., 2008)

Phenotype Manifest In

eye, with Scer\GAL4^ey.PU

(Koh et al., 2012, Griswold et al., 2008)

eye, with Scer\GAL4^GMR.PU

(Griswold et al., 2008)

eye disc | third instar larval stage, with Scer\GAL4^GMR.PU

(Griswold et al., 2008)

ommatidium, with Scer\GAL4^ey.PU

(Koh et al., 2012)

wing, with Scer\GAL4^A9

(Griswold et al., 2008)

Detailed Description

Statement

Reference

Flies expressing Sir2^Scer\UAS.cGa under the control of Scer\GAL4^sNPF-R.2.5 show significantly increased food consumption compared to controls.

(Hong et al., 2012)

Flies with overexpression of Sirt1^Scer\UAS.cGa driven by Scer\GAL4^ey.PU have small eyes with disrupted ommatidial patterns.

(Koh et al., 2012)

Expression of Sir2^Scer\UAS.cGa under the control of Scer\GAL4^GMR.PU results in eyes which lack pigmentation and have a rough appearance. The severity of the phenotype is dose-dependent.

Third larval instar eye discs expressing Sir2^Scer\UAS.cGa under the control of Scer\GAL4^GMR.PU show an increase in cell death compared to controls.

Expression of Sir2^Scer\UAS.cGa under the control of Scer\GAL4^ey.PU results in a small eye phenotype.

Expression of Sir2^Scer\UAS.cGa under the control of Scer\GAL4^A9 results in wing defects.

(Griswold et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^ey.PU, Sirt1^UAS.cGa has visible phenotype, non-enhanceable by Pink1^B9

(Koh et al., 2012)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PU, Sirt1^UAS.cGa has visible phenotype, suppressible by DnaJ-H^UAS.cTa, Scer\GAL4^GMR.PU

(Griswold et al., 2008)

Scer\GAL4^GMR.PU, Sirt1^UAS.cGa has abnormal eye color phenotype, suppressible by DnaJ-H^UAS.cTa, Scer\GAL4^GMR.PU

(Griswold et al., 2008)

Scer\GAL4^GMR.PU, Sirt1^UAS.cGa has visible phenotype, suppressible by Diap1^UAS.cHa, Scer\GAL4^GMR.PU

(Griswold et al., 2008)

Scer\GAL4^GMR.PU, Sirt1^UAS.cGa has abnormal eye color phenotype, suppressible by Diap1^UAS.cHa, Scer\GAL4^GMR.PU

(Griswold et al., 2008)

Scer\GAL4^GMR.PU, Sirt1^UAS.cGa has visible phenotype, suppressible by foxo²⁵/foxo²⁵

(Griswold et al., 2008)

Scer\GAL4^GMR.PU, Sirt1^UAS.cGa has abnormal eye color phenotype, suppressible | partially by foxo²⁵/foxo²⁵

(Griswold et al., 2008)

Scer\GAL4^GMR.PU, Sirt1^UAS.cGa has visible phenotype, suppressible by puc^UAS.cMa/Scer\GAL4^GMR.PU

(Griswold et al., 2008)

Scer\GAL4^GMR.PU, Sirt1^UAS.cGa has abnormal eye color phenotype, suppressible by puc^UAS.cMa/Scer\GAL4^GMR.PU

(Griswold et al., 2008)

Scer\GAL4^GMR.PU, Sirt1^UAS.cGa has visible phenotype, suppressible by bsk^DN.UAS.cUa/Scer\GAL4^GMR.PU

(Griswold et al., 2008)

Scer\GAL4^GMR.PU, Sirt1^UAS.cGa has abnormal eye color phenotype, suppressible by bsk^DN.UAS.cUa/Scer\GAL4^GMR.PU

(Griswold et al., 2008)

Scer\GAL4^GMR.PU, Sirt1^UAS.cGa has visible phenotype, suppressible | partially by Df(3L)H99/+

(Griswold et al., 2008)

Scer\GAL4^GMR.PU, Sirt1^UAS.cGa has abnormal eye color phenotype, suppressible | partially by Df(3L)H99/+

(Griswold et al., 2008)

Scer\GAL4^GMR.PU, Sirt1^UAS.cGa has visible phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PU

(Griswold et al., 2008)

Scer\GAL4^GMR.PU, Sirt1^UAS.cGa has abnormal eye color phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PU

(Griswold et al., 2008)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PU, Sirt1^UAS.cGa has visible phenotype, non-suppressible by p53^Ct.GUS/Scer\GAL4^GMR.PU

(Griswold et al., 2008)

Scer\GAL4^GMR.PU, Sirt1^UAS.cGa has abnormal eye color phenotype, non-suppressible by p53^Ct.GUS/Scer\GAL4^GMR.PU

(Griswold et al., 2008)

Scer\GAL4^GMR.PU, Sirt1^UAS.cGa has visible phenotype, non-suppressible by p53^259H.GUS/Scer\GAL4^GMR.PU

(Griswold et al., 2008)

Scer\GAL4^GMR.PU, Sirt1^UAS.cGa has abnormal eye color phenotype, non-suppressible by p53^259H.GUS/Scer\GAL4^GMR.PU

(Griswold et al., 2008)

Suppressor of

Statement

Reference

Sirt1^UAS.cGa/Scer\GAL4^arm.PU is a suppressor | partially of abnormal locomotor behavior | adult stage phenotype of Pink1^B9

(Koh et al., 2012)

Sirt1^UAS.cGa/Scer\GAL4^arm.PU is a suppressor | partially of increased cell death | adult stage phenotype of Pink1^B9

(Koh et al., 2012)

NOT Suppressor of

Statement

Reference

Sirt1^UAS.cGa/Scer\GAL4^arm.PU is a non-suppressor of abnormal locomotor behavior | adult stage phenotype of park¹

(Koh et al., 2012)

Sirt1^UAS.cGa/Scer\GAL4^arm.PU is a non-suppressor of increased cell death | adult stage phenotype of park¹

(Koh et al., 2012)

foxo²⁵, foxo²¹, Sirt1^UAS.cGa, Scer\GAL4^arm.PU is a non-suppressor of abnormal locomotor behavior | adult stage phenotype of Pink1^B9

(Koh et al., 2012)

foxo²⁵, foxo²¹, Sirt1^UAS.cGa, Scer\GAL4^arm.PU is a non-suppressor of increased cell death | adult stage phenotype of Pink1^B9

(Koh et al., 2012)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Scer\GAL4^ey.PU, Sirt1^UAS.cGa has eye phenotype, non-enhanceable by Pink1^B9

(Koh et al., 2012)

Scer\GAL4^ey.PU, Sirt1^UAS.cGa has ommatidium phenotype, non-enhanceable by Pink1^B9

(Koh et al., 2012)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PU, Sirt1^UAS.cGa has eye phenotype, suppressible by DnaJ-H^UAS.cTa, Scer\GAL4^GMR.PU

(Griswold et al., 2008)

Scer\GAL4^GMR.PU, Sirt1^UAS.cGa has eye phenotype, suppressible by Diap1^UAS.cHa, Scer\GAL4^GMR.PU

(Griswold et al., 2008)

Scer\GAL4^GMR.PU, Sirt1^UAS.cGa has eye phenotype, suppressible | partially by foxo²⁵/foxo²⁵

(Griswold et al., 2008)

Scer\GAL4^GMR.PU, Sirt1^UAS.cGa has eye phenotype, suppressible by puc^UAS.cMa/Scer\GAL4^GMR.PU

(Griswold et al., 2008)

Scer\GAL4^GMR.PU, Sirt1^UAS.cGa has eye phenotype, suppressible by bsk^DN.UAS.cUa/Scer\GAL4^GMR.PU

(Griswold et al., 2008)

Scer\GAL4^GMR.PU, Sirt1^UAS.cGa has eye phenotype, suppressible | partially by Df(3L)H99/+

(Griswold et al., 2008)

Scer\GAL4^GMR.PU, Sirt1^UAS.cGa has eye phenotype, suppressible by BacA\p35^UAS.cHa, Scer\GAL4^GMR.PU

(Griswold et al., 2008)

NOT suppressed by

Statement

Reference

Scer\GAL4^GMR.PU, Sirt1^UAS.cGa has eye phenotype, non-suppressible by p53^Ct.GUS/Scer\GAL4^GMR.PU

(Griswold et al., 2008)

Scer\GAL4^GMR.PU, Sirt1^UAS.cGa has eye phenotype, non-suppressible by p53^259H.GUS/Scer\GAL4^GMR.PU

(Griswold et al., 2008)

Suppressor of

Statement

Reference

Sirt1^UAS.cGa/Scer\GAL4^arm.PU is a suppressor | partially of adult thorax phenotype of Pink1^B9

(Koh et al., 2012)

Sirt1^UAS.cGa/Scer\GAL4^arm.PU is a suppressor | partially of mitochondrion | adult stage phenotype of Pink1^B9

(Koh et al., 2012)

Sirt1^UAS.cGa/Scer\GAL4^arm.PU is a suppressor | partially of indirect flight muscle cell phenotype of Pink1^B9

(Koh et al., 2012)

Sirt1^UAS.cGa/Scer\GAL4^arm.PU is a suppressor | partially of wing blade phenotype of Pink1^B9

(Koh et al., 2012)

NOT Suppressor of

Statement

Reference

foxo²⁵, foxo²¹, Sirt1^UAS.cGa, Scer\GAL4^arm.PU is a non-suppressor of indirect flight muscle cell phenotype of Pink1^B9

(Koh et al., 2012)

foxo²⁵, foxo²¹, Sirt1^UAS.cGa, Scer\GAL4^arm.PU is a non-suppressor of wing blade phenotype of Pink1^B9

(Koh et al., 2012)

Sirt1^UAS.cGa/Scer\GAL4^arm.PU is a non-suppressor of adult thorax phenotype of park¹

(Koh et al., 2012)

Sirt1^UAS.cGa/Scer\GAL4^arm.PU is a non-suppressor of mitochondrion | adult stage phenotype of park¹

(Koh et al., 2012)

Sirt1^UAS.cGa/Scer\GAL4^arm.PU is a non-suppressor of indirect flight muscle cell phenotype of park¹

(Koh et al., 2012)

Sirt1^UAS.cGa/Scer\GAL4^arm.PU is a non-suppressor of wing blade phenotype of park¹

(Koh et al., 2012)

foxo²⁵, foxo²¹, Sirt1^UAS.cGa, Scer\GAL4^arm.PU is a non-suppressor of adult thorax phenotype of Pink1^B9

(Koh et al., 2012)

foxo²⁵, foxo²¹, Sirt1^UAS.cGa, Scer\GAL4^arm.PU is a non-suppressor of mitochondrion | adult stage phenotype of Pink1^B9

(Koh et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Pink1^B9/Y does not enhance eye phenotypes seen in Scer\GAL4^ey.PU>Sirt1^Scer\UAS.cGa flies.

Expression of Sirt1^Scer\UAS.cGa driven by Scer\GAL4^arm.PU partially suppresses phenotypes (collapsed thorax, downturned wings, swollen mitochondria along with increased cell death and reduced levels of mtDNA and ATP in the indirect flight muscle and locomotor defects) seen in (3 day old) Pink1^B9/Y flies. Presence of foxo²⁵/foxo²¹ almost completely nullifies the suppressive effect of Scer\GAL4^arm.PU>Sirt1^Scer\UAS.cGa on phenotypes in Pink1^B9/Y flies.

(Koh et al., 2012)

Co-expression of either th^Scer\UAS.cHa or DnaJ-H^Scer\UAS.cTa suppresses the defective eye phenotype caused by expression of Sir2^Scer\UAS.cGa under the control of Scer\GAL4^GMR.PU.

Co-expression of either p53^Ct.GUS or p53^259H.GUS does not suppress the defective eye phenotype caused by expression of Sir2^Scer\UAS.cGa under the control of Scer\GAL4^GMR.PU.

A significant recovery of eye pigmentation and structure is seen in flies expressing Sir2^Scer\UAS.cGa under the control of Scer\GAL4^GMR.PU in a foxo²⁵/foxo²⁵ background.

Co-expression of either puc^Scer\UAS.cMa or bsk^{DN.Scer\UAS.cUa} ameliorates the eye defects caused by expression of Sir2^Scer\UAS.cGa under the control of Scer\GAL4^GMR.PU.

Df(3L)H99/+ partially rescues the eye defects caused by expression of Sir2^Scer\UAS.cGa under the control of Scer\GAL4^GMR.PU.

(Griswold et al., 2008)

Xenogenetic Interactions

Statement

Reference

Co-expression of BacA\p35^Scer\UAS.cHa suppresses the defective eye phenotype caused by expression of Sir2^Scer\UAS.cGa under the control of Scer\GAL4^GMR.PU.

(Griswold et al., 2008)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Sir2^Scer\UAS.cGa

Sirt1^Scer\UAS.cGa

Sirt1^UAS.cGa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Griswold

Secondary FlyBase IDs

References (6)

Report Sections

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