W95term | foxo-PB; W95term | foxo-PC; W95term | foxo-PF; W95term | foxo-PG; W95term | foxo-PH
G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation. (exact site of mutation unspecified). Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
Presence of foxo25/foxo21 significantly suppresses increased survival in response to rotenone or paraquat in Trap1EY21851/Trap1EY21851 flies. foxo21/+ partially suppresses rescue of locomotor defects by Trap1EY21851/Trap1EY21851 in Pink1B9/Pink1B9 flies. Presence of foxo25/foxo21 suppresses rescue via feeding of G-TTP of locomotor defects in Pink1B9/Pink1B9 flies.
The defect in climbing ability and reduction in lifespan seen in flies expressing forP1.Scer\UAS under the control of Scer\GAL4elav.Switch.PO in the presence of RU486 is partially suppressed by foxo21/+.
Expression of Sirt1Scer\UAS.cGa driven by Scer\GAL4arm.PU partially suppresses phenotypes (collapsed thorax, downturned wings, swollen mitochondria along with increased cell death and reduced levels of mtDNA and ATP in the indirect flight muscle and locomotor defects) seen in (3 day old) Pink1B9/Y flies. Presence of foxo25/foxo21 almost completely nullifies the suppressive effect of Scer\GAL4arm.PU>Sirt1Scer\UAS.cGa on phenotypes in Pink1B9/Y flies.
foxo25/foxo21 does not significantly enhance phenotypes (collapsed thorax, downturned wings, swollen mitochondria along with increased cell death and reduced levels of mtDNA and ATP in the indirect flight muscle and locomotor defects) seen in Pink1B9/Y flies.
A futschK68/+ background suppresses the increase in type 1b bouton area observed in foxo21 homozygotes. Furthermore, this background dominantly suppresses the elevated number of microtubule loops present in foxo21 mutants.
Overexpression of ThorLL.Scer\UAS under the control of Scer\GAL4Mhc.PW in a heterozygous foxo21 mutant genetic background is sufficient to significantly extend longevity compared with wild type by increasing the median and maximum life span of flies.
Ectopic expression of ThorScer\UAS.cTa, under the control of Scer\GAL4Act5C can completely suppress the sensitivity of foxo21/foxo25 flies to oxidative stress (median life-span of 56.8 hours, 39.7% survival rate after 60 hours exposure to 5% hydrogen peroxide).
foxo21/+ significantly partially suppresses the increased cell death induced by expression of Hsap\APLP1Scer\UAS.cMa driven along the anterior/posterior compartment boundary in third instar larval wing discs by Scer\GAL4ptc-559.1, suppresses the blistered wing phenotype in flies with expression of Hsap\APLP1Scer\UAS.cMa driven by Scer\GAL4sd-SG29.1, and suppresses the small scutellum phenotype seen with expression of Hsap\APLP1Scer\UAS.cMa driven by Scer\GAL4pnr-MD237.
Expression of foxoScer\UAS.cFa under the control of Scer\GAL4Lk6-DJ634 rescues the circadian arrhythmia seen in foxo21/foxo25 mutants. Phototaxis is not affected by paraquat treatment in these mutants.